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Method for extracting golden buckwheat high polymeric proanthocyanidin and preparing oligomeric proanthocyanidin by carrying out catalytic degradation on same

A technology for high-polymer procyanidins and an extraction method, which is applied in the field of extraction and catalytic degradation of golden buckwheat high-polymer procyanidins to prepare oligomeric procyanidins, can solve problems such as the degradation method of golden buckwheat high-polymer procyanidins and the like, and achieves a high yield. Effect

Inactive Publication Date: 2009-09-30
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the degradation method of high-polymerization proanthocyanidins in golden buckwheat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Grinding the traditional Chinese medicine golden buckwheat, the particle size of which is about 1-3 cm, to obtain the crude product of golden buckwheat;

[0022] (2) Extract 200 g of the above-mentioned golden buckwheat crude product with 50% ethanol solution, the ratio of material to solution is 1:6, the temperature is 50°C, and the time is 24 hours;

[0023] (3) Ethanol was evaporated from the extract, water was added to 600ml, centrifuged for 10min after stirring, the rotation speed was 3000rpm, the precipitate was discarded, the supernatant was extracted twice with equal volume of ethyl acetate, and the aqueous phase raffinate was collected;

[0024] (4) Put the aqueous phase raffinate on the LSA-21 macroporous resin column, first wash with 3 column volumes of distilled water, and then elute with 3 column volumes of 50% ethanol solution. The collected eluate was concentrated under reduced pressure and dried to obtain 10.23 g of high-polymeric proanthocyanidins f...

Embodiment 2

[0026] (1) Grinding the traditional Chinese medicine golden buckwheat, the particle size of which is about 1-3 cm, to obtain the crude product of golden buckwheat;

[0027] (2) Extract 300 g of the above-mentioned golden buckwheat crude product with 60% ethanol solution, the ratio of material to solution is 1:10, the temperature is 50° C., and the time is 24 hours;

[0028] (3) Remove ethanol from the extract, add water to 1500ml, centrifuge for 10min after stirring, at a speed of 5000rpm, discard the precipitate, extract the supernatant twice with equal volume of ethyl acetate, and collect the aqueous phase raffinate;

[0029] (4) Put the aqueous phase raffinate on the LSA-21 macroporous resin column, wash with 2 times column volume of distilled water, and then elute with 4 column volumes of 70% ethanol solution. The collected eluate was concentrated under reduced pressure and dried to obtain 12.11 g of high-polymeric proanthocyanidins from golden buckwheat, with a proanthocy...

Embodiment 3

[0031] (1) Grinding the traditional Chinese medicine golden buckwheat, the particle size of which is about 1-3 cm, to obtain the crude product of golden buckwheat;

[0032] (2) Extract 400 g of the above-mentioned golden buckwheat crude product with 50% ethanol solution, the ratio of material to solution is 1:8, the temperature is 50° C., and the time is 24 hours;

[0033] (3) Ethanol was evaporated from the extract, water was added to 1600ml, centrifuged for 10min after stirring, the rotating speed was 5000rpm, the precipitate was discarded, the supernatant was extracted twice with equal volume of ethyl acetate, and the aqueous phase raffinate was collected;

[0034] (4) Put the aqueous phase raffinate on the LSA-21 macroporous resin column, first wash with 2 times column volume of distilled water, and then elute with 5 column volumes of 50% ethanol solution. The collected eluate was concentrated under reduced pressure and dried to obtain 22.24 g of high-polymeric proanthocya...

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Abstract

The invention relates to a method for extracting golden buckwheat high polymeric proanthocyanidin and preparing oligomeric proanthocyanidin by carrying out catalytic degradation on the same. The method comprises the following steps: taking golden buckwheat root tuber as a raw material, taking an aqueous solution of ethanol as a solvent to leach the golden buckwheat root tuber, centrifugating the leaching liquor after volatilizing the ethanol, extracting a supernatant by an organic solvent, passing water phase raffinate through a macroporous resin, washing by an ethanol-water system, collecting an eluted solution, and carrying out decompression concentration and vacuum drying on the eluted solution to obtain a high polymer of the golden buckwheat proanthocyanidin; taking the aqueous solution of the ethanol as a solvent to prepare a solution of the high polymer of the golden buckwheat proanthocyanidin, carrying out an H2 degradation reaction under the high pressure in the presence of a palladium / carbon catalyst, filtering the reaction product, passing the filtrate through a macroporous resin column for adsorption and purification, eluting the aqueous solution of the ethanol, and carrying out decompression concentration and vacuum drying on the eluted solution to obtain the golden buckwheat oligomeric proanthocyanidin. The method can improve the yield of the high activity substance, namely the oligomeric proanthocyanidin, thereby expanding the application range of the golden buckwheat in the fields of medicaments, foods, cosmetics and health care products.

Description

technical field [0001] The invention relates to a method for extracting high-polymeric proanthocyanidins from golden buckwheat and its catalytic degradation to prepare low-polymeric proanthocyanidins. technical background [0002] Proanthocyanidin is a flavonoid polymer, which is a metabolic secondary product of plants. Oligomeric proanthocyanidin has significant effects in anti-cancer, anti-oxidation, anti-mutation, hypoglycemic, anti-inflammatory, pain relief, hemostasis, prevention of arteriosclerosis and prevention of skin allergies. biological function, beneficial to human health. Currently representative sources of proanthocyanidins include grape seeds and the like. Golden buckwheat Fagopyrum cymosum is a medicinal protected plant in the Polygonaceae (Polygonaceae) Buckwheat genus (Fagopyrum). Wild golden buckwheat contains rich excellent genes and is an important traditional Chinese medicinal material in China. In terms of chemical research, the active ingredients a...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 吕敬慈雍克岚施雅陈旭黄晓月
Owner SHANGHAI UNIV
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