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Crossbreeding method of common kidney beans

A technology of crossbreeding and bean, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of small pollen amount and small bean flower, achieve the goal of reducing water loss, reducing workload, and ensuring hybridization rate Effect

Inactive Publication Date: 2015-11-11
毕节市农业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Aiming at the deficiencies of the prior art, the present invention provides a method for hybrid breeding of common bean, which can solve the problem that the bean flower of the existing bean hybrid breeding is very small and the amount of pollen is small

Method used

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  • Crossbreeding method of common kidney beans
  • Crossbreeding method of common kidney beans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Hybridization time selection: choose in the morning on a sunny day;

[0028] 2. Female parent selection: select the flower buds, gently peel off the flag petals with tweezers, and the flowers with slightly green to white keels (3 or 4 days before flowering);

[0029] 3. Male parent selection: It is advisable to choose flowers that will open or have just opened, and the anthers have matured two days before flowering;

[0030] 4. Pollination: Gently support the female parent flower buds with your hands, and use a tip of tweezers to gently scratch the closed place in the middle of the flag petals of the buds, trying not to scratch the flag petals. Use your thumb and forefinger to gently pinch the two ends of the flower bud and press it in the middle. The flag petals will open naturally, exposing the keel petals. Use tweezers to tear the keel petals and pinch out the stamens. There are 10 stamens in total (common bean). Pick up the male parent flower, remove the flag pe...

Embodiment 2

[0034] Use tweezers to peel off the flag petals, wing petals, and lightly clamp the front end of the keel petal to open a small opening at the commissure, then use your hands or tweezers to gently pull the two wing petals backward and downward, so that the stigma is separated from the front end of the keel petal. Stretch out, use the keel flap clipped by the male parent, and insert it into the stigma of the female parent from the opened small mouth to complete pollination. Combining the method of inserting the stigma is simpler and feasible, but the disadvantage of the pull-out stigma method itself cannot be avoided.

[0035] The present embodiment is an example with common bean cross-pollination, Papilionaceae self-pollination plants, such as: tuan bean, mung bean etc., all can use this stigma to be inserted into the keel petal method to carry out cross-pollination. The experimental data are shown in the table below:

[0036]

[0037] Table I

[0038]

[0039] Table I...

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Abstract

The invention discloses a crossbreeding method of common kidney beans, and relates to the field of crossbreeding. The crossbreeding method comprises the following steps: picking the anther and stigma of a male parent carina peridium simultaneously; arranging carina picked from a male parent in a female parent stigma through a small patulous opening in a sleeving manner. The crossbreeding method has the following benefits: 1, the workload is decreased: the anther and stigma of the male parent carina peridium are directly picked simultaneously, so that the steps of obtaining male parent pollen are simplified, and the workload is decreased; 2, the seed setting index is increased: the technical key point is based on the male parent, the male parent carina is complete, all the pollen is in the carina, pollen loss is avoided, the complete male parent carina provides an optimal environment for the female parent stigma, and the optimal environment is similar to the stigma self-pollination environment in a female parent carina, so that water loss of the female parent stigma is reduced, and the probability of pollination success is increased; 3, the hybridizing rate is increased: the female parent stigma is sleeved by the male parent carina, the female parent stigma is enabled to be in contact with the male parent pollen only, and other pollen is isolated, so that the hybridizing rate is guaranteed.

Description

technical field [0001] The invention belongs to the field of hybrid breeding, and in particular relates to a method for hybrid breeding of common bean. Background technique [0002] Kidney bean is a self-pollinating plant. The flowers are butterfly-shaped hermaphroditic flowers. The keels of the bean are covered with pistils. [0003] The prior art scheme relevant to the present invention: [0004] 1. The method of pulling out the stigma: Use tweezers to peel off the flag petals, wing petals, and lightly clamp the front end of the keel petal of the female flower bud to open a small opening at the commissure, and then gently pull the two wing petals backward and downward with hands or tweezers. Make the stigma protrude from the front end of the keel flap, then smear the female stigma with the pollen-bearing brush-like stigma of the male parent to complete pollination; [0005] 2. Putting method: use tweezers to open the flag petals and wing petals, and clamp the front end o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 余莉张时龙杨珊王昭礼赵龙卢运吴宪志陈小翠李清超刘建新马浪浪梁振娟
Owner 毕节市农业科学研究所
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