PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3

Abstract

The invention discloses a PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3, and belongs to the technical field of agro-biological engineering. According to the PCR molecular marking method, four specific molecular marking primers are designed and synthesized for PCR amplification of DNA of different rice based on the difference of long-grain and large-grain variety TD70 and short-grain and small-grain variety Kasalath on the nucleotide base sequence of the long-grain gene qGL3, the amplified product is subjected to electrophoresis detection with 2.0% of agarose gel, and DNA brands of different dimension represent different allele types of qGL3 after DuRed nuclear acid developing. With the adoption of the molecular marking method, the difference of different alleles of the long-grain gene qGL3 in rice germplasm resources or breeding population can be quickly and accurately identified; in addition, the selection efficiency for the long-grain homozygous genotype qGL3-TqGL3-T is obviously improved; and therefore, the coordinative improvement of the appearance quality and output traits of rice can be realized.

Description

1. Technical field [0001] The invention discloses a PCR molecular marker method for identifying rice grain length gene qGL3 allele variation, belongs to the technical field of agricultural bioengineering, and is specially used for qGL3-containing qGL3- T qGL3- T Screening, identification and variety selection of genotype long-grain, high-quality, high-yield rice resources. 2. Background technology [0002] Rice is an important food crop in my country. The annual planting area is about 30 million hectares, accounting for about 30% of the country's grain planting area. The total output of rice exceeds 200 million tons, accounting for more than 40% of the country's total grain output. my country's food security plays an important role in promoting the development of the national economy (Lu Jingbo, China Rice, 2002, 6:17-20). [0003] In recent years, with the growth of population and the improvement of people's living standards, the demand for high-quality rice in the domesti...

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Problems solved by technology

However, the reported linkage markers that can be used for genotype screening of qGL3 all come from indica and japonica cross-mapping populations with large genetic differences. Not only lack of polymorphism in breeding selection, but also it is difficult to guarantee the genotype of individual plants in offspring. Identification accuracy (Zhang X J, et al., Proc Natl Acad Sci USA, 2012, 109(52):21534-21539)

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