PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3

A molecular marker and allele technology, applied in the field of agricultural bioengineering, can solve the problems of lack of polymorphism, identification accuracy of single plant genotype of difficult offspring, etc., to reduce breeding costs, speed up appearance quality and yield, and shorten breeding. effect of cycle

Active Publication Date: 2014-06-25
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

However, the reported linkage markers that can be used for genotype screening of qGL3 all come from indica and japonica cross-mapping populations with large genetic differences. Not only lack of polymorphis

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  • PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3
  • PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3
  • PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3

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[0035] Through the identification and analysis of grain shape traits of more than 3,000 rice germplasm resources in the medium-term bank of agricultural germplasm resources in Jiangsu Province, one was screened from Swan Valley / / / 9520 / / (72-496 / yu Waxy) long and large-grain japonica rice stable line TD70 of the hybrid progeny. By crossing TD70 with short and small-grain Indian indica variety Kasalath, a recombinant inbred line population containing 240 lines was constructed by single-seed propagation method. Several QTLs related to grain shape were mapped on rice chromosomes, and one grain length QTL-qGL3-2 was mapped on chromosome 3. Through the sequencing analysis of TD70 and Kasalath DNA, it was determined that qGL3-2 is the reported qGL3 gene, a new major QTL located on the long arm of rice chromosome 3 that controls grain length ( figure 2 ). Compared with the short and small grain rice variety Kasalath, the long and large grain line TD70 has a single-base C-A mutatio...

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Abstract

The invention discloses a PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3, and belongs to the technical field of agro-biological engineering. According to the PCR molecular marking method, four specific molecular marking primers are designed and synthesized for PCR amplification of DNA of different rice based on the difference of long-grain and large-grain variety TD70 and short-grain and small-grain variety Kasalath on the nucleotide base sequence of the long-grain gene qGL3, the amplified product is subjected to electrophoresis detection with 2.0% of agarose gel, and DNA brands of different dimension represent different allele types of qGL3 after DuRed nuclear acid developing. With the adoption of the molecular marking method, the difference of different alleles of the long-grain gene qGL3 in rice germplasm resources or breeding population can be quickly and accurately identified; in addition, the selection efficiency for the long-grain homozygous genotype qGL3-TqGL3-T is obviously improved; and therefore, the coordinative improvement of the appearance quality and output traits of rice can be realized.

Description

1. Technical field [0001] The invention discloses a PCR molecular marker method for identifying rice grain length gene qGL3 allele variation, belongs to the technical field of agricultural bioengineering, and is specially used for qGL3-containing qGL3- T qGL3- T Screening, identification and variety selection of genotype long-grain, high-quality, high-yield rice resources. 2. Background technology [0002] Rice is an important food crop in my country. The annual planting area is about 30 million hectares, accounting for about 30% of the country's grain planting area. The total output of rice exceeds 200 million tons, accounting for more than 40% of the country's total grain output. my country's food security plays an important role in promoting the development of the national economy (Lu Jingbo, China Rice, 2002, 6:17-20). [0003] In recent years, with the growth of population and the improvement of people's living standards, the demand for high-quality rice in the domesti...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/686C12Q2531/113C12Q2535/125
Inventor 张亚东周丽慧郑佳丁丹王才林陈涛姚姝朱镇赵庆勇赵凌于新赵春芳
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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