The invention discloses a method for determining the large karst spring replenishment channel and intensity. The method comprises the following steps of 1, using a spring water position to judge the sensitivity of a rainfall station; 2, determining the main runoff zone depth and distribution thereof; 3, verifying the main runoff zone depth and distribution thereof determined in the step 2 througha temperature and conductivity tracer technique; 4, calculating the intensities of different runoff replenishment sources. By means of the method for determining the large karst spring replenishment channel and intensity, on the basis of the nature of a regional hydrogeological condition, through the four elements of the relevance of the rainfall station and the spring water position, the karst fissure rate, the temperature tracer and the water chemical mixing ratio, the outdoor large-scale karst spring replenishment channel is precisely identified, a scientific basis is provided for protection of large karst spring water flow and water quality, and decision-making support is provided for related government departments to manage groundwater resources.