SNP (single nucleotide polymorphism) site and CAPS (cleaved amplified polymorphic sequence) mark interlocked with citrullus lanatus fruit bitter taste gene Bt (bitterness)

A technology of fruit bitterness gene and watermelon, which is applied in the direction of DNA preparation, recombinant DNA technology, DNA / RNA fragments, etc., can solve the problems of large impact on production, difficulty in identification of fruit bitterness, no fruit bitterness gene found, etc., and achieve the effect of shortening the time

Active Publication Date: 2013-07-10
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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Problems solved by technology

[0004] In addition, the fruit bitterness of other crops of Cucurbitaceae, such as cucumber, is more serious and has a greater impact on production. It is

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  • SNP (single nucleotide polymorphism) site and CAPS (cleaved amplified polymorphic sequence) mark interlocked with citrullus lanatus fruit bitter taste gene Bt (bitterness)
  • SNP (single nucleotide polymorphism) site and CAPS (cleaved amplified polymorphic sequence) mark interlocked with citrullus lanatus fruit bitter taste gene Bt (bitterness)
  • SNP (single nucleotide polymorphism) site and CAPS (cleaved amplified polymorphic sequence) mark interlocked with citrullus lanatus fruit bitter taste gene Bt (bitterness)

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Embodiment 1

[0028] This embodiment is about the Bt-linked SNP site of the watermelon fruit bitterness gene and the CAPS marker, as well as the method for obtaining the marker.

[0029] The watermelon fruit bitterness gene Bt-linked SNP site and CAPS marker have the nucleotide base sequences of SEQ ID NO: 1-2 in the sequence list.

[0030] In the sequence listing, SEQ ID NO:1 is the nucleotide sequence specifically amplified by the primer pair Bt-F and Bt-R for non-bitter materials, with a total of 503 bases;

[0031] In the sequence listing, SEQ ID NO: 2 is the nucleotide sequence specifically amplified by the primer pair Bt-F and Bt-R to the fruit bitter material, with a total of 503 bases;

[0032] The acquisition method includes the following steps:

[0033] A, selection of test materials: the test materials include male parent, female parent, F1 generation, RILs population, resequencing material, natural population;

[0034] The male parent is: PI296341FR, which is a typical wild ty...

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Abstract

The invention belongs to the fields of a gene sequence and an obtaining method of the gene sequence, and particularly relates to an obtaining method of an SNP (single nucleotide polymorphism) site and a CAPS (cleaved amplified polymorphic sequence) mark interlocked with a citrullus lanatus fruit bitter taste gene Bt (bitterness). The SNP site and the CAPS mark interlocked with the citrullus lanatus fruit bitter taste have nucleotide base sequences in SEQ ID NO:1-2 in a sequence table. The citrullus lanatus fruit bitter taste gene is primarily positioned by screening a bitter taste single plant in an RILs colony and combining with the high-density genetic map information of the citrullus lanatus; the candidate SNP site interlocked with the character is combined with separate colony verification analysis and natural colony verification analysis; the mark tightly interlocked with a target character is obtained; the SNP site and the CAPS mark can be applied to improvement of citrullus lanatus variety and molecular assisted breeding; a technical support is provided for molecular breeding of the citrullus lanatus quality; and meanwhile, the traditional gene positioning time is greatly shortened.

Description

technical field [0001] The invention belongs to the field of gene sequences and methods for obtaining them, and in particular relates to methods for obtaining SNP sites linked to watermelon fruit bitterness (Bt) and CAPS markers. Background technique [0002] Watermelon (Citrullus lanatus) is an important crop of Cucurbitaceae, accounting for 7% of the world's vegetable crop area, and the global annual output is about 90,000,000 tons (http: / / faostat.fao.org). my country's watermelon production and sales rank first in the world, and it is an important horticultural crop with international competitiveness and large economic growth space in my country. The recent release of the whole genome sequence of watermelon has greatly promoted the research on molecular biology of watermelon and provided a more solid foundation for molecular design-assisted breeding. With the advent of the watermelon genome era, further clarification of gene functions and finding key genes that control i...

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Application Information

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IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 许勇张洁张海英宫国义郭绍贵任毅
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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