SNP loci linked with blight resistant gene Fon-1 in watermelon, and markers thereof

Abstract

The invention relates to the fields of gene sequences and acquiring methods thereof, and concretely relates to SNP loci linked with a blight resistant gene Fon-1 in a watermelon, markers thereof, and acquiring methods of the loci and the markers. The SNP loci linked with a blight resistant gene Fon-1 in a watermelon and the markers thereof have nucleotide base sequences represented by SEQ ID NO:1-8 in a sequence table. The acquiring method of the loci comprises the following steps: 1, selecting a tested material; 2, carrying out preliminary positioning of the blight resistant gene Fon-1 in the watermelon; 3, acquiring candidate SNP; and 4, acquiring the SNP loci closely linked with the blight resistant gene Fon-1 in the watermelon, and verifying the candidate SNP loci. According to the invention, the phenotype identification and the molecular detection of F2 generation separation populations and natural populations are carried out through utilizing a series of markers to confirm the close linkage degree of the series of the SNP loci with target properties; and the SNP loci are utilized to design the markers respectively, and the markers can be utilized to carry out the initial-stage screening of the kind in order to reach a molecule assisted breeding purpose, so the breeding period is substantially shortened, and an important scientific research meaning is possessed.

Description

technical field [0001] The invention belongs to the field of gene sequences and methods for obtaining them, in particular to SNP sites and markers linked to the watermelon fusarium wilt resistance gene Fon-1 and methods for obtaining them. Background technique [0002] Watermelon belongs to Cucurbitaceae Cutrullus lanatus, which is an important economic crop widely cultivated at home and abroad. my country's watermelon has a millennium planting history and high economic value. It is one of the important economic crops to increase farmers' income today. It has become an important horticultural crop with international competitiveness and large economic growth space in my country. According to statistics from the Food and Agriculture Organization of the United Nations, my country's annual watermelon production accounts for 67% of the world's total, and it is also the country with the largest per capita consumption of watermelon in the world. [0003] Watermelon Fusarium wilt i...

AI Technical Summary

Problems solved by technology

[0005] As far as the existing technology is concerned, the main defect that needs to be overcome to obtain the closely linked SNP loci and markers of the watermelon Fusarium wilt physiological race 1 resistance gene is the identification of the watermelon Fusarium wilt seedling stage phenotype, which is difficult to determine the watermelon Fusarium wilt phenotype , largely affected by the environment and other pests and diseases, the accuracy of phenotypic identification directly affects the positioning of the Fon-1 gene and the acquisition of molecular markers closely linked to it; Relying on genome information, it is necessary to perform sequence comparison on the resequencing of the resistance and susceptibility traits determined by fusarium wilt in order to obtain candidate SNP sites that match the phenotype more quickly, and finally develop the resistance gene Fon Tightly linked markers of -1

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