The invention discloses fritillaria superfine powder as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, selecting materials and cleaning; step 2, drying and sterilizing; step 3, grinding and pulping; step 4, pre-freezing slurry; step 5, freezing and drying; step 6, smashing for multiple times into the fritillaria superfine powder with a grain size of 0.5-2 [mu]m. The fritillaria superfine powder is prepared by the preparation method. The fritillaria superfine powder prepared by the preparation method is taken as an only active ingredient to be applied to the preparation of a drug for treating a pulmonary disease. The fritillaria superfine powder disclosed by the invention is used for treating pneumothorax, pulmonary bullous, emphysema, pulmonary shadow, lung cancer, pulmonary heart disease, respiratory failure, pulmonary embolism, pulmonary abscess, pneumonia, neonatal pneumonia, infantile pneumonia, trachitis, asthma, pulmonary tuberculosis, pneumoconiosis and / or interstitial lung disease and has the advantages that the treatment effect is obvious, the condition of a patient is stable, the life quality of the patient is improved, the weight of the patient is increased, the immune function of the patient is improved, and the medication is safe.