Reagent box and method for genome extraction on automatic nucleic acid extraction workstation

Abstract

The invention discloses a reagent kit for extracting genome on an automated nucleic acid extraction workstation, and a method thereof, wherein, the reagent kit contains a lysis buffer, a guanidinium buffer, a bead and an eluent; the method comprises the steps of: disintegrating biological tissues or cells by using the lysis buffer, adsorbing DNA by using the bead in the reagent kit, removing impurity by using a cleaning solution and finally recycling the DNA by using the eluent to finish the extraction of the DNA. A regular PCR, a fluorescent quantitation PCR, Genotyping, STR and other various follow-up processing operations can be carried to the extracted DNA. The reagent kit and the method of the invention have the advantages of having wide sample applicability of the reagent, being easy to be operated, and requiring no centrifugalization processing in the whole process, thus being beneficial to the advance of the follow-up operation; the invention can cooperate with the automated nucleic acid extraction workstation to carry out effective DNA extraction and can be used in gene extraction with manual operation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit and a method for effectively extracting genomic DNA at an automated nucleic acid extraction workstation. Background technique [0002] Nucleic acid extraction from biological tissues or cells is now a very mature technology with low technical content and mostly repetitive work, while nucleic acid extraction using workstations is a new type of technology, which uses processed magnetic beads The principle of DNA adsorption can be effectively carried out in a certain solution environment, and the workstation is used to extract DNA from the genome. It is characterized by high work efficiency, can simultaneously extract 96 channels, and greatly improves the repeatability of the extraction work. . At present, the genome extraction using the workstation method has been widely used in industries such as blood banks, agriculture, and hospital inspection centers, and the market capacity...

AI Technical Summary

Problems solved by technology

At present, the genome extraction using the workstation method has been widely used in industries such as blood banks, agriculture, and hospital inspection centers, and the market capacity is relatively large. However, there are currently only two CTAB and SBS methods, which can cooperate with automated nucleic acid extraction workstations for nucleic acid extraction. Reagents, these reagents There are defects such as poor versatility, complicated operation steps, and high prices.

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