Kit and method for fast separating virus nucleic acid from biological sample

A virus nucleic acid and kit technology, applied in the field of nucleic acid separation, can solve the problems of poor versatility, expensive viral nucleic acid extraction kits, etc.

Inactive Publication Date: 2011-05-25
GENMAG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commercialized magnetic bead virus nucleic acid extraction kits are expensive, and the versatility between different instruments is poor. There is an urgent need to develop cheap, efficient, stable and simple virus nucleic acid extraction kits

Method used

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  • Kit and method for fast separating virus nucleic acid from biological sample
  • Kit and method for fast separating virus nucleic acid from biological sample
  • Kit and method for fast separating virus nucleic acid from biological sample

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1: the extraction of HEVRNA

[0044] Serum samples containing HEV were taken for 10-fold serial dilution, and then HEV RNA was extracted with this kit, and Chemagen’s viral nucleic acid extraction kit was used as a control. Follow the steps below to extract HEV RNA:

[0045] (1) Take 100 μl of the serially diluted HEV-containing serum sample and put it in a centrifuge tube, add 5ul (20μg / μl) ProteinaseK and 100μl of lysis buffer, mix well, and place at 55°C for 10 minutes.

[0046] (2) Add 20 μl of magnetic bead suspension (shake well before use) to the above mixture, mix thoroughly, and mix gently and thoroughly at room temperature for 10 minutes.

[0047] (3) Place the centrifuge tube on a magnetic stand, magnetically separate for 30 seconds, and discard the supernatant.

[0048] (4) Add 250 μl of washing buffer I, oscillate to fully suspend the magnetic beads, separate with a magnetic stand after 1 minute, and discard the supernatant.

[0049] (5) Add 2...

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Abstract

The invention discloses a kit and a method for fast separating virus nucleic acid from a biological sample, in order to fast separate virus nucleic acid from a cell-free biological sample. The kit disclosed by the invention comprises a lysis buffer solution, a magnetic bead, a washing buffer solution and an elution buffer solution. The method disclosed by the invention comprises the steps of: disintegrating a biomaterial so that the magnetic bead and the nucleic acid form a complex, separating the complex under the action of an externally-applied magnetic field, removing impurities by washing, and then eluting and recovering the nucleic acid. The method for extracting the nucleic acid is simple, convenient and safe in operation, low in cost, intact in nucleic acid and high in purity, and the nucleic acid can be directly applied to subsequent experiments. The kit can be used for efficiently extracting the virus nucleic acid in combination with an automated nucleic acid extracting instrument and can also be used for extracting the nucleic acid by means of manual operation.

Description

technical field [0001] The present invention relates to methods for isolating nucleic acids from biological samples. Specifically, the present invention provides a kit and method for rapidly purifying viral nucleic acid from a cell-free biological sample. Background technique [0002] At present, nucleic acid purification is inseparable from many research fields in biomedicine. Efficient and simple separation and purification from biological materials to obtain complete and high-purity target nucleic acids is the basis for the study of viral diseases. [0003] Traditional nucleic acid purification methods are cumbersome, time-consuming, and low in extraction efficiency, making it difficult to meet the needs of high-throughput experiments. At the same time, the traditional method uses reagents such as phenol / chloroform to produce huge toxic effects on the human body and pollute the environment. Magnetic beads can quickly separate nucleic acids under the action of an extern...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 赵海峰
Owner GENMAG BIOTECH
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