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Method and device for treating sample and reagent

A technology of samples and reagents, applied in the field of dry processing

Inactive Publication Date: 2003-04-09
阿尔法赫里克斯有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not possible to program a set of extraction procedures, buffer exchanges, sequential extractions, etc. in a convenient and reliable manner.

Method used

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  • Method and device for treating sample and reagent
  • Method and device for treating sample and reagent
  • Method and device for treating sample and reagent

Examples

Experimental program
Comparison scheme
Effect test

example

[0059] In the present example, a set of injectors that can be connected to each other is used. Experiment with different substrates: silica particles, glass and nylon fibers. A dissolution buffer solution containing saline solution and detergent is used. Wash buffer solutions include saline solution and ethanol.

[0060] A sample of non-heparinized human whole blood is drawn into the first syringe, either directly from the patient using a hypodermic needle attached to the syringe, or from an intermediate container containing the patient sample, such as a Vacutainer  , to extract.

[0061] A second syringe containing silica particles suspended in the dissolution buffer solution is provided. Then, the syringe containing the blood sample is connected to the second syringe, and the blood sample is expelled into the lysis buffer solution. The mixed contents of the two syringes are then pumped back and forth between the two syringes. The narrow mid-section of the interconnecte...

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Abstract

Nucleic acids are extracted rapidly, safely and directly from a sample without pipetting steps by using predispensed, interconnectable vessels. These vessels are used separately or interconnected according to the mictrotitre standard format. The sample is mixed with lysis buffer and the nucleic acids bound to a matrix in a closed system, comprising at least two interconnectable volumes. By forcing the sample and buffer mixture back and forth from one volume to another, passing a narrow passage, thorough mixing is ensured.

Description

technical field [0001] The present invention relates to near or so-called dry processing of reagents and samples, in particular to sample preparation, quantitative and qualitative extraction, from organic samples such as blood, serum, urine, cell suspensions, samples amplified in test tubes and The field of purification and amplification of nucleic acid (DNA or RNA, including various nucleic acids) in biopsy samples. Background technique [0002] There are a large number of different protocols for isolating and purifying nucleic acids. Most methods aim at isolating highly purified samples suitable for PCR amplification. [0003] A currently used scheme, using Split Second  The DNA preparation equipment (manufactured by Boehringer Mannheim GmbH) includes the following steps: preparing the first buffer solution in a microcentrifuge tube. A sample, such as human whole blood, is added to the tube. The tube was placed on a rocking platform for 10 minute...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09A61K48/00B01F11/00B01F13/00B01L3/00B01L3/02B01L3/14C12M1/00C12N15/00C12Q1/68C12Q1/686G01N33/48
CPCB01L2400/0409B01L3/5082B01F15/0225B01L2400/0478B01L2200/026B01L2200/0631B01F13/002B01L2300/0681B01F11/0082B01L3/0217B01L2300/042C12Q1/686B01L3/563B01F31/441B01F33/5011B01F35/7163C12Q2547/101
Inventor M·马尔姆奎斯特
Owner 阿尔法赫里克斯有限公司
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