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Full-scale C-reactive protein (CRP) colloidal gold immunoturbidimetric assay kit

A technology for detecting kits and reactive proteins, which is applied in biological testing, material inspection products, etc. It can solve the problems of unfavorable cleaning of biochemical instruments, high manufacturing cost of latex, high price of latex-enhanced immune turbidimetric kits, and achieve great market competitiveness. , high sensitivity, easy to clean effect

Active Publication Date: 2012-10-24
重庆沃康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Moreover, after the reaction of latex-enhanced immunoturbidimetric method, precipitation will occur, which is not conducive to the cleaning of the biochemical instrument, and the manufacturing cost of latex is relatively high, resulting in a high price of latex-enhanced immunoturbidimetric kit.

Method used

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  • Full-scale C-reactive protein (CRP) colloidal gold immunoturbidimetric assay kit
  • Full-scale C-reactive protein (CRP) colloidal gold immunoturbidimetric assay kit
  • Full-scale C-reactive protein (CRP) colloidal gold immunoturbidimetric assay kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Kit preparation 1:

[0028] (1) The preparation of reagent R1 is as follows: add BSA with a final concentration of 0.5% (w / v) to 20mmol / L Tris buffer (pH7.5), and add BSA with a final concentration of 2% (w / v) PEG20000, NaN3 was added to a final concentration of 0.09% (w / v). Reagent R1 was thus prepared.

[0029] (2) The preparation of colloidal gold is as follows:

[0030] a) Cleaning of the glass container: Rinse it with tap water, then soak it with 1% to 2% hydrochloric acid overnight, take it out and rinse it with plenty of tap water, then wash it with detergent and rinse it with running water, then soak it with distilled water overnight, and then carry out colloid Jin Run wash.

[0031] b) Prepare 1% chloroauric acid and 1% sodium citrate solution, filter with 0.2um filter membrane.

[0032] c) Take a 1000ml round bottom flask, put it into the rotor, and add 500ml ultrapure water.

[0033] d) Add 5ml of 1% chloroauric acid, 600rpm, heat until the solution boil...

Embodiment 2

[0043] Kit preparation 2:

[0044] (1) Preparation of reagent R1

[0045] Add BSA at a final concentration of 2% (w / v) to 10 mmol / L citrate buffer (pH6.5), add PEG20000 at a final concentration of 0.5% (w / v), and add a final concentration of 0.09% ( w / v) NaN3. Thus the R1 reagent was prepared.

[0046] (2) Preparation of colloidal gold:

[0047] a) Cleaning of the glass container: Rinse it with tap water, then soak it with 1% to 2% hydrochloric acid overnight, take it out and rinse it with plenty of tap water, then wash it with detergent and rinse it with running water, then soak it with distilled water overnight, and then carry out colloid Jin Run wash.

[0048] b) Prepare 1% chloroauric acid and 1% sodium citrate solution, filter with 0.2um filter membrane.

[0049] c) Take a 1000ml round bottom flask, put it into the rotor, and add 500ml ultrapure water.

[0050] d) Add 5ml of 1% chloroauric acid, 600rpm, heat until the solution boils, immediately absorb 4ml of 1% sod...

Embodiment 3

[0060] Kit preparation 3

[0061] (1) Preparation of reagent R1

[0062] Add BSA at a final concentration of 1% (w / v) to 10 mmol / L phosphate buffer (pH6.5), add PEG20000 at a final concentration of 1% (w / v), and add a final concentration of 0.09% (w / v) / v) NaN3. Thus the R1 reagent was prepared.

[0063] (2) Preparation of colloidal gold:

[0064] a) Cleaning of the glass container: Rinse it with running water, then soak it with 1% to 2% hydrochloric acid overnight, take it out and rinse it with a large amount of tap water, then wash it with detergent and rinse it with running water, then soak it with distilled water overnight, and then carry out colloid Jin Run wash.

[0065] b) Prepare 1% chloroauric acid and 1% sodium citrate solution, filter with 0.2um filter membrane.

[0066] c) Take a 1000ml round bottom flask, put it into the rotor, and add 500ml ultrapure water.

[0067] d) Add 5ml of 1% chloroauric acid, 600rpm, heat until the solution boils, immediately absorb...

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Abstract

The invention provides a full-scale CRP colloidal gold immunoturbidimetric assay kit. A quantitative assay purpose is reached through enhancing the turbidity by the colloidal gold. The method used by the kit provided by the invention solves a problem that the generation of a precipitate after a latex reinforced immunoturbidimetric reaction goes against biochemical instrument cleaning. The kit provided in the invention comprises a reagent R1 and a reagent R2, wherein the reagent R2 is a proper buffer solution; and the reagent R2 is a buffer solution of the colloidal gold combined with an antihuman CRP antibody. The kit has the characteristics of high sensitivity, strong specificity and good stability, can be used for assaying the content of the CRP in serum or blood plasma, and is suitable for clinical fully-automatic biochemical analyzers. The CRP assay sensitivity of the kit can reach 0.01mg / L, and the upper CRP assay limitation of the kit is 500mg / L.

Description

technical field [0001] The invention relates to a colloidal gold-enhanced immune turbidimetric detection kit; more specifically, it relates to a full-range C-reactive protein immune turbidimetric detection kit. Background technique [0002] C-reactive protein (C-reactive protein) is a special protein produced by liver cells in the human body, which is an indicator of inflammatory response; C-reactive protein has a molecular weight of 115KD and consists of five identical terminal glycosylated polypeptide subunits Composition, each subunit contains 187 amino acids, these subunits are linked by non-covalent bonds to form a ring-shaped pentamer, and there is an interchain disulfide bond. [0003] C-reactive protein was first discovered in the serum of patients with acute lobar pneumonia in 1930 by Tillett and Francis et al. 2+ A substance that, when present, reacts specifically with C-polysaccharides in the cell wall of pneumococci. In 1941, Avery et al. determined that it was...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 徐彩霞叶佳颖刘献文孙火明
Owner 重庆沃康生物科技有限公司
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