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Water-dispersible multicolour fluorescent polymer nanoparticles and preparation method thereof

A fluorescent polymer and nanoparticle technology, applied in chemical instruments and methods, luminescent materials, etc., can solve the problems of harsh synthesis conditions, unfavorable expansion of production and application, etc., achieve simple synthesis route, improve structure and photostability, and huge application. Foreground effect

Inactive Publication Date: 2014-01-08
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to factors such as harsh synthesis conditions and limitations in the use of dyes, this method is not conducive to further commercialization and expansion of production and application.

Method used

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  • Water-dispersible multicolour fluorescent polymer nanoparticles and preparation method thereof
  • Water-dispersible multicolour fluorescent polymer nanoparticles and preparation method thereof
  • Water-dispersible multicolour fluorescent polymer nanoparticles and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1: Preparation of water-dispersible blank polymer nanoparticles (NP-00) by miniemulsion polymerization.

[0033] Stir the mixture of 0.625g methyl methacrylate and 0.059g n-hexadecane on an ordinary magnetic stirrer for 5 minutes to obtain solution A; meanwhile, add 0.025g of SDS to 10ml of water and stir on an ordinary magnetic stirrer Solution B was obtained in 10 minutes. After mixing solutions A and B, stir at room temperature for 15-20 minutes to form a pre-emulsion, put the pre-emulsion into a 650W power ultrasonic cell pulverizer in an ice-water bath (keep below 20°C), and ultrasonically emulsify for 15 minutes. A stable miniemulsion can be obtained. The miniemulsion was transferred into a 25ml round bottom flask, 0.022g potassium persulfate was added to initiate polymerization, and the round bottom flask was placed in a 75 oC oil bath for 3h. After the reaction, cool to room temperature, and simply filter to obtain the desired water-dispersible blank pol...

Embodiment 2

[0034] Example 2: Miniemulsion Polymerization Preparation of water-dispersible fluorescent polymer nanoparticles (NP-A1) containing a single EANI fluorescent dye.

[0035] Stir the mixture of 0.625g methyl methacrylate, 0.059g n-hexadecane and 0.00094g of EANI on a common magnetic stirrer for 5 minutes to obtain solution A; at the same time, add 0.025g of SDS to 10ml of water. Stir on a magnetic stirrer for 10 minutes to obtain solution B. After mixing solutions A and B, stir at room temperature for 15-20 minutes to form a pre-emulsion, put the pre-emulsion into a 650W power ultrasonic cell pulverizer in an ice-water bath (keep below 20°C), and ultrasonically emulsify for 15 minutes. A stable miniemulsion can be obtained. The miniemulsion was transferred into a 25ml round bottom flask, 0.022g potassium persulfate was added to initiate polymerization, and the round bottom flask was placed in a 75 oC oil bath for 3h. After the reaction, cool to room temperature, and simply fil...

Embodiment 3

[0036] Example 3: Miniemulsion polymerization preparation of water-dispersible multicolor fluorescent polymer nanoparticles (NP-B1, NP-B2 and NP-B3) containing different ratios of EANI and NBDAA fluorescent dyes.

[0037] 0.625g methyl methacrylate, 0.059g n-hexadecane, 0.00094g EANI, 0.00074g (corresponding to sample NP-B1) or 0.00148g (corresponding to sample NP-B2) or 0.00222g (corresponding to sample NP-B2) -B3) The NBDAA mixture was stirred on a common magnetic stirrer for 5 minutes to obtain solution A; at the same time, 0.025 g of SDS was added to 10 ml of water, and stirred on a common magnetic stirrer for 10 minutes to obtain solution B. After mixing solutions A and B, stir at room temperature for 15-20 minutes to form a pre-emulsion, put the pre-emulsion into a 650W power ultrasonic cell pulverizer in an ice-water bath (keep below 20°C), and ultrasonically emulsify for 15 minutes. A stable miniemulsion can be obtained. The miniemulsion was transferred into a 25ml ro...

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Abstract

The invention discloses water-dispersible multicolour fluorescent polymer nanoparticles and a preparation method thereof. The water-dispersible multicolour fluorescent polymer nanoparticles are prepared by taking methyl methacrylate as a monomer, taking 4-ethyoxyl-9-allyl-1,8-naphthalimide (EANI), 4-amido-7-nitro-N-allylbenzo[1,2,5] oxadiazole (NBDAA), and Nile red methacrylate (NRME) as polymerizable fluorescent dyes, taking lauryl sodium sulphate (SDS) as a surfactant, and using a one-step miniemulsion polymerization method. The water-dispersible multicolour fluorescent polymer nanoparticles are great in dispersibility in water, quite high in fluorescent brightness, and capable of emitting multicolour fluorescence from blue to red under the excitation of a single wave. The water-dispersible multicolour fluorescent polymer nanoparticles obtained by the preparation method disclosed by the invention are uniform in size, and good in structure and light stability, has a fluorescence emission signal capable of being adjusted for many times in a wide interval under a single excitation wavelength, is simple in synthesis route, convenient to use, suitable for amplification synthesis and actual production applications, and has a great application prospect in the fields of biological encoding and multiple biological analysis.

Description

technical field [0001] The invention relates to the field of material preparation, in particular to a method for preparing water-dispersible multicolor fluorescent polymer nanoparticles with multiple adjustable fluorescence emission signals under single-wavelength excitation. Background technique [0002] With the continuous development of biomedical technology, multiple bioanalysis methods are playing an increasingly important role in many applications such as genetic testing, high-throughput screening of new drugs, and clinical diagnosis. Typically, multiplexed bioassays require the use of many multicolor fluorescent probes with distinguishable fluorescent signals under excitation at a single wavelength. However, the number of traditional organic small molecule fluorescent probes capable of meeting this requirement is very limited. In addition, there are still some insurmountable defects in the application of traditional organic fluorescent dyes, such as photobleaching of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/14C08F226/06C08F226/02C08F220/36C08F2/26C09K11/06
Inventor 陈建黄富华李亚
Owner HUNAN UNIV OF SCI & TECH
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