System and method for quantitative detection of test strips on basis of continuous fluorescent-substance markers

Abstract

The invention belongs to the field of bio-medical instruments, and in particular relates to a system and a method for quantitative detection of test strips on the basis of continuous fluorescent-substance markers. The system comprises a continuous fluorescent-substance marker test strip, a test strip frame, a lighting system, an imaging system, a fluorescent image receiver, a signal amplifier, ananalog / digital converter, a data processing-controlling system, an output display device, a printer, a keyboard and an IC card matched with the test strip. The data processing-controlling system reads parameters of the IC card and then controls the test strip frame to move so as to ensure that the light emitted by the lighting system is reflected via a dichroic mirror and then automatically scansthe test strip; acquired characteristic wavelength reflection fluorescence is transmitted to the data processing-controlling system for optical density identification and concentration calculation via the fluorescent image receiver, the signal amplifier and the analog / digital converter; and the output display device displays results. The invention can quickly and accurately realize the quantitative or qualitative detection of single-component and multi-component samples. The system has the characteristics of high detection sensitivity, objective results, flexible use and the like.

Description

technical field [0001] The invention relates to the field of biomedical devices, in particular to a test strip quantitative detection system based on continuous fluorescent substance labeling and a method thereof. The system can automatically scan and interpret the test strips marked with fluorescent substances, and realize the detection of pathogens, antigens, antibodies, prohibited drugs, major diseases (tumor, cancer, cardiovascular disease and diabetes, etc.), pesticide residues, food, etc. Quantitative or qualitative detection of various target objects such as safety biological detection. Background technique [0002] In immunoassay, methods such as radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA) and colloidal gold labeling are currently mainly used to achieve quantitative or qualitative detection of samples. These methods have various defects. Laser-induced fluorescence detection technology is considered to be one of the most sensitive detection met...

AI Technical Summary

Problems solved by technology

The disadvantages are: (1) The patent application number only describes the production of quantum dot marking test strips, but does not disclose the corresponding supporting instrument detection system. The detection needs to be carried out with the help of other ultraviolet detectors, and it is only a qualitative detection , cannot achieve quantitative detection

(2) The use of ultraviolet detection is harmful to the human body and unsafe to operate

(3) The objectivity of the measurement results is poor

Since the tester judges the test result by observing the fluorescence intensity of the test strip and the quality control zone under ultraviolet light with the naked eye, the test result is easily affected by the tester's experience and is highly subjective. The same test strip is in different states. It is possible to make different or even wrong judgment results, especially for those samples with extremely low target content, the fluorescence emitted by ultraviolet light is very weak, it is more difficult to judge the results with the naked eye, and the sensitivity is low

(4) Cannot monitor the dynamic process of biological reactions

Immunochromatography is a dynamic reaction process. The fluorescence produced by the quantum dot label particles in the siphon migration state on the test strip belongs to the dynamic category. Obviously, the ultraviolet visual method cannot observe and analyze this dynamic reaction process, so it is impossible to go deeper. To study the immunochromatographic reaction process

Even if the biological reaction fails, the detector cannot analyze the reason for the failure of the reaction well

Chinese patent application No. 200610024566.3 discloses a method of combining quantum dot fluorescent probes to detect biochips to find Chinese medicine targets. This patent also has shortcomings: the detection of the "quantum dot-Chinese medicine ingredients" fluorescent probes involved not only requires other technologies to provide Corresponding protein chips, and its detection also requires additional special equipment

Chinese patent application number 200510059892.3 discloses an optoelectronic rapid diagnostic test system including quantum dot test strip detection, but this patent has not yet developed a ready-made product, and the content is completely different from the present invention

It should also be pointed out that there have been many literature reports on solid-phase detection methods using rare earth nanoparticles as continuous fluorescent markers to label microwell plates, and a corresponding matching detector—time-resolved fluorescence detector (or system) has been developed. ), although its detection can achieve high sensitivity and can achieve quantitative or semi-quantitative detection, but the disadvantage is that the operation is complicated, involving multiple steps such as adding samples, warming bath, washing, color development, termination, detection, etc., and the operation takes 2-4 hours In order to complete the process, there are certain requirements for the operator and the environment. Moreover, in addition to the need for an expensive supporting instrument——time-resolved detector——it also needs other auxiliary equipment such as a plate washer. Therefore, its general application is still limited.

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