Synthetic method and application of polydopamine-modified carbon nanotube composite material

A technology of carbon nanotubes and composite materials, applied in the analysis of materials, material analysis by electromagnetic means, instruments, etc., can solve the problems of MALDI-MS signal instability, uneven carbon nanotubes, and inability to disperse uniformly, achieving excellent results Environmental stability and biocompatibility, good biocompatibility, low cost effect

Inactive Publication Date: 2013-04-03
FUDAN UNIV
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Problems solved by technology

However, carbon nanotubes have a hydrophobic outer wall, which is easy to agglomerate and settle in the solution, and cannot be uniformly dispersed, and the carbon nanotubes on the target are not uniform, resulting in unstable MALDI-MS signals, poor experimental repeatability, and easy contamination of the sample target. Formation of sample "hot spots" on

Method used

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  • Synthetic method and application of polydopamine-modified carbon nanotube composite material
  • Synthetic method and application of polydopamine-modified carbon nanotube composite material
  • Synthetic method and application of polydopamine-modified carbon nanotube composite material

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Synthesis of polydopamine-modified carbon nanotube composites

[0028] (1) Disperse 10mg of carbon nanotubes in 40ml of aqueous solution containing 40mg of dopamine, and sonicate for 30 minutes.

[0029] (2) Under continuous magnetic stirring, quickly add 10ml of tris buffer solution (10mM, pH 8.5) to the product obtained in step (1).

[0030] (3) Continue mechanical stirring at room temperature for 10 hours.

[0031] (4) Wash the product three times each with deionized water and ethanol, and centrifuge.

[0032] (5) Dry in a vacuum oven at 50°C.

[0033] figure 1 Scanning electron micrographs of carbon nanotubes; figure 2 It is a scanning electron micrograph of the carbon nanotube composite material of the surface-modified polydopamine obtained in Example 1; image 3 is a transmission electron micrograph of carbon nanotubes; Figure 4 It is a transmission electron micrograph of the carbon nanotube composite material of the surface-modified polydopamine obtained...

Embodiment 2

[0034] Example 2: Carbon nanotube composites as substrates for high-throughput matrix-assisted laser desorption ionization mass spectrometry analysis of small metabolites

[0035] (1) Sample preparation: prepare 1 mg / mL suspensions of carbon nanotubes and carbon nanotube composite materials respectively, the solvent ratio is water: ethanol = 1:1 (v / v), and sonicate. The metabolites and small molecular compounds to be detected are all accurately weighed and prepared into a 0.1 mg / ml solution. Among them, sucrose, glucose, histidine, threonine and methionine are dissolved in water, and stearic acid and arachidic acid are dissolved in a mixed solution of water and ethanol (volume ratio is 1:1).

[0036] (2) Spot target: pipette 1 μL of carbon nanotubes and carbon nanotube composites on the MALDI target plate, and place them in the air at room temperature to dry naturally to form a thin matrix layer; take 1 μL of the analyte solution and drop it on the matrix layer and let it dry...

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Abstract

The invention belongs to the field of nanotechnology, and particularly relates to a synthetic method and application of a polydopamine-modified carbon nanotube composite material. The synthetic method comprises the following steps in sequence: dispersing carbon nanotubes into dopamine-containing aqueous solution; quickly adding trismetyl aminomethane buffer solution into the dopamine-containing aqueous solution in which the carbon nanotubes are uniformly dispersed under magnetically stirring; mechanically stirring for 10 hours at room temperature; washing by water; and centrifugally separating to obtain the carbon nanotube composite material of which the surface is modified by polydopamine, wherein the carbon nanotube composite material shows high dispersibility in water. The polydopamine-modified carbon nanotube composite material adopts the carbon nanotubes as the framework, and therefore, a large specific surface area is provided; the polydopamine-modified carbon nanotube composite material is excellent in environmental stability and biocompatibility and high in dispersibility in water; the synthetic method is simple and low in cost; and the polydopamine-modified carbon nanotube composite material can be used as the substrate for analyzing metabolite micromolecule under high-throughput MALDI-TOFMS (Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry).

Description

technical field [0001] The invention belongs to the field of advanced nanomaterials and nanotechnology, and specifically relates to a synthesis method and application of a polydopamine-modified carbon nanotube composite material used for high-throughput MALDI-TOF MS analysis of metabolite small molecules. Background technique [0002] Small metabolite molecules play a vital role in living organisms. Through the detection of small metabolite molecules, a lot of information related to living organisms can be obtained. There are many methods for the detection of metabolite small molecules, among which chromatography methods have been widely used. However, due to the need to derivatize small metabolites during analysis by chromatographic methods, the complexity of sample pretreatment is increased. Matrix-assisted laser desorption ionization mass spectrometry provides a high-throughput, high-sensitivity, and fast analytical method. Among them, the matrix plays an important rol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00G01N27/62
Inventor 邓春晖史辰漪
Owner FUDAN UNIV
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