The invention relates to a micro-ecological preparation for biocontrol on tomato anthracnose and a preparation method of the micro-ecological preparation and belongs to the technical field of anthracnose prevention and treatment. The micro-ecological preparation provided by the invention comprises the following raw materials in parts by weight: 80-100 parts of a composite microorganism bacterial agent, 3-5 parts of a plant methanol extract, 8-10 parts of a salt solution, 3-4 parts of glucose, 0.2-0.5 part of polyaspartic acid, 0.1-0.3 part of heteroauxin, 10-15 parts of fruit juice and 20-30 parts of water. The preparation method of the micro-ecological preparation comprises the following steps: compounding microorganism colonies, extracting plant methanol, preparing the salt solution, andthe like. By adopting the micro-ecological preparation, a biological prevention and treatment method is adopted, antagonism and inhibition functions of microorganisms are utilized, bacteria are treated and inhibited with bacteria, the richness of microorganism colonies on surfaces of tomato surfaces is maintained, environment pollution caused by chemical pesticides is reduced, the plant growth vigor is improved, and the ecological benefits are increased.