The invention discloses a single-fiber-type CARS excitation source device and realization method based on two-stage non-linear tuning. The device comprises a femtosecond fiber laser light source, an electric control light power attenuator, a first-stage tuner, a pulse broadening device, a second-stage tuner, a pump laser, a wavelength division multiplexer, a gain fiber, an optical filter and an output port. The femtosecond fiber laser light source outputs femtosecond pulses serving as Stokes optical pulses which enter the electric control light power attenuator; power control optical pulse wavelength tuning is realized in the first-stage tuner; the pulses are broadened to be femtosecond pulses through the pulse broadening device, and the femtosecond pulses then enter the gain fiber for amplification; signal optical pulses and idler frequency optical pulses are generated in the second-stage tuner, wherein the signal optical pulses serve as pump optical pulses of a CARS source; the optical filter filters the idler frequency optical pulses and the residual pump continuous light; and the rest optical pulses are output from the output port. Compared with the prior art, the device adopts a single-fiber light path structure and electric control tuning, and has the advantages of compact structure, anti-interference, high reliability and fast tuning speed; and CARS rapid imaging requirement is met.