The invention discloses a spot test method and a test device for fuel cell stack membrane electrode conditions. Hydrogen and nitrogen or air are supplied to to-be-tested fuel cells, fuel cell voltage is removed through loads until zero, constant current charging is carried out on the to-be-tested fuel cells by adopting a constant-current power supply, a charging current is measured by a current sensor, current signals of the current sensor and voltage signals of each of the to-be-tested fuel cells are collected by a data collector, converted into digital quantity signals and transmitted to a data processing unit, automatic processing of measured data is achieved through program writing by the data processing unit, and parameters such as catalytic agent effective and active area, double-layer capacitance, hydrogen permeation current and impedance of membrane electrodes of the to-be-tested fuel cells are provided through differential and integral operation on collected voltage data of each fuel cell. The spot test method and the test device for the fuel cell stack membrane electrode conditions are suitable for testing a fuel cell stack or a single fuel cell, and has the advantages of being spot, free of damage, simple and convenient, fast and the like.