Degradation strain JN7 of petroleum hydrocarbons in oily sludge and application thereof

A JN7, petroleum hydrocarbon technology, applied in the field of bioremediation, can solve the problems of no large-scale application, large influence of environmental factors, long treatment period, etc., to avoid complex reaction products, optimize soil microbial system, and enhance biological activity.

Active Publication Date: 2018-05-15
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using metabolic surfactant microorganisms to treat oily sludge can increase the solubility of petroleum hydrocarbons, which can improve the degradation efficiency of petroleum hydrocarbons, and use biological treatment to remove harmful substances such as PAHs. Biological treatment can simultaneously degrade aliphatic hydrocarbons and aromatics in oily sludge However, the biological treatment method has l

Method used

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  • Degradation strain JN7 of petroleum hydrocarbons in oily sludge and application thereof
  • Degradation strain JN7 of petroleum hydrocarbons in oily sludge and application thereof
  • Degradation strain JN7 of petroleum hydrocarbons in oily sludge and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Degradation and remediation test of oily sludge in Liaohe Oilfield

[0060] Take oily sludge from Liaohe Oilfield, air-dry, grind, pass through a 1mm sieve, and set aside. Pick a single colony into sterilized LB liquid medium, seal it with a breathable parafilm, and place the conical flask in a shaker for activation at 120 rpm and 30 °C for 24 h. Weigh 40 g of the above-mentioned oily sludge after air-drying, grinding and sieving, add the activated bacterial solution of Pseudomonas sp.JN7 with OD600=1 according to 10% of the inoculum, and adjust the water-soil ratio to 2:5 with distilled water, and seal with a breathable sealing film. All conical flasks were placed in a constant temperature incubator at 30°C for static culture, and samples were taken every 5 days. The total petroleum hydrocarbon content in the oily sludge was measured by an infrared spectrophotometer. The degradation curve is shown in the appendix. image 3 shown. It can be seen from the fi...

Embodiment 2

[0064] Example 2: Degradation effect of Pseudomonas sp.JN7 on petroleum hydrocarbons in oily sludge at different pH values

[0065] Oily sludge from Liaohe Oilfield was taken, air-dried, ground, and passed through a 1mm sieve. Weigh 40 g of the above-mentioned oily sludge into a conical flask, adjust the pH of the soil to 1, 3, 5, 7, 9, and 11 with dilute H2SO4 and NaOH, and add Pseudomonas sp. The bacterial solution activated by JN7 was adjusted with distilled water to adjust the water-soil ratio to 2:5, and sealed with a breathable sealing film. All the conical flasks were placed in a constant temperature incubator at 30°C for static culture and samples were taken after 25 days, and the content of total petroleum hydrocarbons in the oily sludge was measured by an infrared spectrophotometer. The degradation effects of strain Pseudomonas sp.JN7 on petroleum hydrocarbons in oily sludge under different pH conditions are as follows: Figure 4 shown. Depend on Figure 4 It can...

Embodiment 3

[0066]Example 3: Degradation effect of Pseudomonas sp.JN7 on petroleum hydrocarbons in oily sludge at different temperatures

[0067] Oily sludge from Liaohe Oilfield was taken, air-dried, ground, and passed through a 1mm sieve. Weigh 40 g of the above-mentioned oily sludge into a conical flask, add Pseudomonas sp.JN7 activated bacterial solution with OD600=1 according to 10% of the inoculum, adjust the water-soil ratio to 2:5 with distilled water, and seal with a breathable sealing film. The conical flasks were placed in a constant temperature incubator at 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, and 50°C for static culture, and samples were taken after 25 days. Determination of total petroleum hydrocarbons in oily sludge with a spectrometer. The degradation effects of strain Pseudomonas sp.JN7 on total petroleum hydrocarbons in oily sludge at different temperatures are as follows: Figure 5 shown. Depend on Figure 5 It can be seen that the strain of the present i...

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Abstract

The invention discloses an efficient degradation strain JN7 of petroleum hydrocarbons in oily sludge and application thereof, and belongs to the technical field of biological restoration. The degradation strain JN7 is preserved in CGMCC (China General Microbiological Culture Collection Center), has the preservation number of CGMCC NO.14978, and has the classification number of Pseudomonas sp.. Thepurpose of the strain JN7 with the petroleum hydrocarbon degradation function is the oily sludge control performed in a petroleum hydrocarbon degradation mode. The degradation rate of the bacterium in 30 days on the TPH in the oily sludge is 50.75 percent; the singe strain of the bacterium has the obvious degradation capability on n-C10 to n-C30 petroleum hydrocarbons; GC-MS analysis shows that the petroleum hydrocarbon substances in oily sludge take oxidization reaction under the effect of the strain to form a great number of phenol and ketone type intermediate products. The bacterium has the most proper degradation conditions of the temperature being 20 to 50 DEG C and the pH value being 7 to 11 on petroleum contaminants. The biological method is used for the restoration technology of the oily sludge, and has the advantages of high efficiency, low cost, environment-friendly effect and the like.

Description

technical field [0001] The invention relates to the technical field of bioremediation, in particular to a strain for degrading and restoring oily sludge, and its application in degrading oily sludge. Background technique [0002] Since the 20th century, oil has become one of the most important energy sources for human beings. While being exploited and used in large quantities, the harm caused by oil and its processed products to the environment and human beings is becoming more and more serious. Since the reform and opening up, my country's annual crude oil output has exceeded 100 million tons, and China has become one of the top ten oil-producing countries in the world. China has more than 400 oil fields and oil and gas fields that are explored and developed, accounting for about 3% of the country's land area. Province (city) and autonomous region. However, in the process of rapid economic and technological development and rising demand for oil, our technology for oil recov...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F11/02C02F101/32C12R1/38
CPCC02F11/02C02F2101/32C12N1/205C12R2001/38
Inventor 叶正芳姜楠赵泉林
Owner PEKING UNIV
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