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A kind of oil-degrading bacteria in oily sludge and its application

A technology of degrading bacteria and CNPC, applied in the field of microorganisms, can solve the problems of complex components of oily sludge, less research on microbial degradation and remediation, and difficult to degrade

Active Publication Date: 2017-09-12
TIANJIN UNIV OF SCI & TECH
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  • Claims
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Problems solved by technology

Due to the complex composition of oily sludge, which is difficult to degrade, there are relatively few researches on microbial degradation and remediation of oily sludge at home and abroad.

Method used

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  • A kind of oil-degrading bacteria in oily sludge and its application
  • A kind of oil-degrading bacteria in oily sludge and its application
  • A kind of oil-degrading bacteria in oily sludge and its application

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Embodiment Construction

[0018] The present invention will be described in further detail below through specific examples. The following examples are only descriptive and not restrictive, and the protection scope of the present invention cannot be limited by this.

[0019] This application uses the following methods to screen and confirm the types of strains, while providing various excellent properties of the strains. The details are as follows:

[0020] 1Materials and methods

[0021] 1.1 Oily sludge

[0022] The oily sludge samples were derived from drilling debris from the Bohai Oilfield and were taken from the Environmental Protection Technology Service Branch of CNOOC Bohai Company. The water content of the sludge was determined to be 70.55%, pH 6.4, and salinity 3.0%.

[0023] 1.2 Medium

[0024] 1.2.1 Enrichment and separation medium (g / L) Yeast powder 3, NaCl 5, MgSO 4 ·7H 2 O 0.25, (NH 4 ) 2 SO 4 1, K 2 HPO 4 10, KH 2 PO 4 4. pH 7.2~7.4.

[0025] 1.2.2 Slant solid medium LB solid medium.

[0026] 1.2....

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Abstract

The present invention relates to a petroleum-degrading bacterium in oily sludge. The degrading bacterium is preserved in CGMCC, General Microorganism Center of China Microbial Strain Preservation Management Committee, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Chinese Academy of Sciences, Deposit number: CGMCC8647, classification number is Corynebacterium glutamicum. This application isolates oil-degrading bacteria (RS1) from oily sludge in Bohai Oilfield, and its preservation number is CGMCC8647. It has been proved by experiments that RS1 has a high degradation rate of saturated hydrocarbons, reaching 20.74%. The degradation rate of total petroleum hydrocarbons (TPH) was 39.69%. The optimal conditions for the bacteria to degrade petroleum were 37°C, salinity 0.5%-5%, and pH6-8.

Description

Technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain that degrades petroleum hydrocarbons, in particular to a petroleum-degrading bacteria in oily sludge and its application. Background technique [0002] Oil is one of the indispensable energy sources in today's social life and production, and it is also one of the most serious environmental pollutants recognized globally. A large amount of oily sludge is generated during the exploration, development, processing and transportation of petroleum. Oily sludge usually contains alkanes, aromatic hydrocarbons, non-hydrocarbon compounds and asphaltenes, which threaten human health through soil and groundwater pollution of the ecological environment. Therefore, the safe disposal of oily sludge is a major issue. The treatment and disposal of oily sludge mainly involves the reduction of oil content and the separation of water and oil sludge. The current treatment methods such as incineratio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/15C02F103/10C02F101/32
Inventor 刘宪斌任丽君田胜艳赵兴贵
Owner TIANJIN UNIV OF SCI & TECH
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