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51 results about "District hospital" patented technology

A hospital is a health care institution providing patient treatment with specialized medical and nursing staff and medical equipment. The best-known type of hospital is the general hospital, which typically has an emergency department to treat urgent health problems ranging from fire and accident victims to a sudden illness. A district hospital typically is the major health care facility in ...

Intelligent analysis and assessment system for disease management in hospital

ActiveCN104951894AImprove satisfactionAddress structural issuesResourcesDiseaseDynamic contrast
The invention discloses an intelligent analysis and assessment system for disease management in a hospital. A user inquiry terminal is used for in-hospital hospital lean management and contrast assessment among hospitals in terms of four dimensions including medical treatment quality, medical treatment efficiency, medical treatment benefit and satisfaction of patients according to assessment results of a hospital management quality assessment module. Various screening, free combination and 360-degree omni-directional dynamic contrast among different dimensions are realized, various screening, combination and omni-directional contrast in the same dimension are also realized, problems of hospital management, ranking and positioning are made clear by big data professional analysis of the hospital, hospital management policy supporting and hospital management contrast in different dimensions, comprehensive contrast and ranking in terms of ICD (international classification of diseases) diagnosis, diagnosis related grouping (DRG) diseases, clinical doctors, clinical departments and hospitals in a region are realized, advantages and disadvantages of contrasted objects in the industry are made clear, clear positioning can be realized, lean management of the hospital is promoted, and comprehensive competition capacity of the hospital is improved.
Owner:中科厚立信息技术(成都)有限公司

Intelligent health management system

The invention discloses an intelligent health management system. The intelligent health management system is characterized by comprising an intelligent hospital system, a regional health system and a family health system. Through the intelligent health management system, intercommunication and sharing of medical information and / or health information between families, communities, hospitals, medical scientific research institutions and health supervision and management departments can be achieved, and the intelligent health management system can deal with mass data processing.
Owner:新疆一心代码互联网科技有限公司

Portable detection device

The invention relates to a portable detection device, comprising a detection box, a detection chip, a smartphone and mobilephone software. When the portable detection device is in use, a to-be-detected sample is added on the detection chip; then the detection chip is inserted into the detection box; the smartphone is put in the device according to indication after a period of time; the mobilephone software is started for detection; and detection results are given out by the mobilephone software. The smartphone is a phone daily used by a user, so that only the mobilephone software is needed to install. The device is low in detection cost, fast in speed, easy to carry and convenient for operation. The device overcomes the defects of time consumption and high cost in detection of professional medical institutions, and is suitable for households, travel users, community hospitals, food detection institutions, etc.
Owner:BEIJING BAIKANGXIN BIOLOGICAL SCI & TECH

Time resolution fluorescence immunochromatography test strip and kit for jointly detecting CA19-9 and Carcino-Embryonic Antigen (CEA)

The invention relates to a time resolution fluorescence immunochromatography test strip for jointly detecting CA19-9 and a Carcino-Embryonic Antigen (CEA). The test strip comprises a base plate, a nitrocellulose membrane, a glass fiber cotton and water absorption paper, wherein the nitrocellulose membrane is coated with a CA19-9 monoclonal antibody used as a test 1 wire (T1 wire), a CEA monoclonalantibody used as a test 2 wire (T2 wire) and a sheep anti-mouse polyclonal antibody used as a quality control wire (C wire); the glass fiber cotton is divided into a sample application region and a combination region; the combination region is sprayed with a time resolution fluorescence microsphere-labeled CA19-9 monoclonal antibody compound and a CEA monoclonal antibody compound. According to the time resolution fluorescence immunochromatography test strip and the kit for jointly detecting the CA19-9 and the CEA, test for the concentrations of the CA19-9 and the CEA can be completed within arelatively short time; a test result is high in accuracy and high in sensitivity; a requirement for quick test beside a clinical bed and requirements of a primary community hospital can be met.
Owner:河南省生物工程技术研究中心

Medical service supply chain platform and collaborative management method thereof

The invention discloses a medical service supply chain platform comprising a registration appointment module through which a registration appointment service is provided by a system APP, a telecommunication operator and a mobile communication operator; a test module through which a test service is provided by all levels of hospitals and medical scientific research institutes participating in collaboration; a preliminary diagnosis / diagnosis / treatment module through which corresponding services are provided by all levels of hospitals, including community service centers, district hospitals, municipal hospitals and provincial hospitals, and all types of specialized hospitals participating in collaboration; a medicine dispensing module through which the services are provided by all levels and all types of pharmacies and all levels and all types of hospital pharmacies; and a recovery / follow-up module through which the services are provided by the community medical service centers. The beneficial effects of the medical service supply chain platform are that the level of the medical service is enhanced and medical cost is reduced by effective collaboration between different medical service providers as for the internal part of a medical service supply chain.
Owner:SHANGHAI DEXIN MEDICAL TECH SERVICE CO LTD

Novel coronavirus detection kit

The invention discloses a novel coronavirus detection kit. The novel coronavirus detection kit comprises antigen detection test paper. The antigen detection test paper comprises a lining, absorbent paper, an immune nitrocellulose membrane and an immune microsphere pad, wherein the immune microsphere pad, the immune nitrocellulose membrane and the absorbent paper are adhered to the lining. The immune microsphere pad is coated with a novel coronavirus 2019-nCoV monoclonal antibody 1 marked by latex microspheres; and the immune nitrocellulose membrane is provided with a detection line coated witha novel coronavirus 2019-nCoV monoclonal antibody 2 and a quality control line coated with a goat anti-mouse IgG polyclonal antibody. According to the invention, latex particles are used as a markingtracer, and a target object is detected and analyzed by using an antigen-antibody reaction and a lateral chromatography method. The kit has the advantages of convenience and quickness in actual operation, detection personnel do not need to detect professional knowledge, detection experiments do not need professional equipment configuration, and the kit can be widely used in primary community hospitals and health service centers.
Owner:BEIJING JINWOFU BIOENGINEERING TECH CO LTD

In situ hybridization detection kit for mRNA level at precancerous stage of breast cancer, detection method and application thereof

The invention discloses an in situ hybridization detection kit, which comprises a hybridization probe and a marker. The invention further discloses a method for in situ hybridization detection of an mRNA level of a zinc finger protein 703 (ZNF703) gene closely related with pathological evolution at a precancerous stage of breast cancer by using the kit. The method comprises the following steps that: (1) under a condition that the hybridization probe and a target sequence can form a stable hybrid complex, the RNA requiring detection in the substrate contacts the hybridization probe to form a hybrid complex; and (2) the hybrid complex is detected. With the kit and the detection method of the present invention, the expression amount of the ZNF703 gene on the mRNA level can be detected, such that the provision time of the index is earlier than the provision time of the index by medical imaging or by existing clinical biochemical detection, the screening on the mRNA level at the precancerous stage can be achieved truly. In addition, the detection method of the present invention has characteristics of simpleness, convenience and low cost, and is applicable for popularization and application in county hospitals and district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

Novel emergency medical modular isolation unit and construction method thereof

InactiveCN112878748AAir safetyConvenient living and dietary needsWallsHospitalsPatient roomWhole body
The invention discloses a novel emergency medical modular isolation unit and a construction method thereof. The novel emergency medical modular isolation unit comprises a main body frame, a medical-grade isolation wall body material, a sterilization and purification device and electrical equipment. According to the novel emergency medical modular isolation unit and the construction method thereof, factory prefabrication and flow production line manufacturing are adopted, batch production can be achieved, the novel emergency medical modular isolation unit can be put into use only through foundation construction and fixing after being transported to a site, the single unit is small in occupied area and convenient and fast to use, and not only a community medical isolation requirement can be met, but also a temporary isolation inpatient area hospital can be assembled by arranging connecting nodes; a plurality of layers of medical-grade isolation wall body materials are adopted in the whole body, so that a medical grade is achieved; an efficient fresh air sterilization technology is adopted in the module, an isolation ward is under a negative pressure, and a sterilization filter unit at an external air outlet is matched to ensure air safety inside and outside the ward; and a disinfection device for an ultraviolet sterilization and purification delivery window not only can be convenient for life and diet of isolated people, but also is safe and reliable.
Owner:MCC TIANGONG TIANJIN EQUIP MFG +1

Oral health management system

The invention discloses an oral health evaluation system. The system comprises a portable mobile device, a management device and a medical evaluation software platform. The portable mobile device is carried by a user and can be used for receiving and sending health data and consulting requests at any time; the management device is server electronic equipment and is used for storing information such as health records of the user; and the medical evaluation software platform performs personalized evaluation on the oral health data of each user. According to the system, medical information and / orhealth information can be intercommunicated and shared among families, communities, hospitals, medical scientific research institutions and health supervision and management departments, and mass data can be processed in real time.
Owner:煜宜(上海)健康管理有限公司

In situ hybridization detection kit for lung cancer , detecting method and use thereof

The invention relates to an in-situ hybridization comprehensive detection reagent kit for lung cancer, which comprises four groups of hybridization probes and markers, wherein the hybridization probes have sequences shown in a sequence table SEQ ID NO.1, a sequence table SEQ ID NO.2, a sequence table SEQ ID NO.3, and a sequence table SEQ ID NO.4 respectively. The invention also provides an in-situ hybridization detection method for a PAX9 gene, a NKX2-8 gene, a TTF1 gene and a RHOGDI2 gene. In addition, the invention also provides application of the reagent kit to preparation of a medicine for detecting early diagnosis, metastasis and recurrence of the lung cancer. The reagent kit has the advantages that the reagent kit has the characteristics of high sensitivity and strong specificity. The detection method has convenient and simple operation, and can be universally used and promoted in district hospitals and above.
Owner:SUZHOU FUYING GENE TECH

Leukemia lesion prophase mRNA level in situ hybridization detection kit, detection method and applications thereof

The present invention discloses an in situ hybridization detection kit, which comprises a hybridization probe and a marker. The present invention further discloses a method for in situ hybridization detection of mRNA of transcription factor (TWIST) gene closely related with leukemia lesion prophase pathological evolution by using the kit, wherein the method comprises the following steps that: (1) RNA requiring detection in a substrate contacts a hybridization probe under a certain condition to form a hybridization complex, wherein the condition is that the hybridization probe and a target sequence can form a stable hybridization complex; and (2) the hybridization complex is detected. With the kit and the detection method, an expression level of the TWIST gene at an mRNA level can be detected so as to provide an early diagnosis compared with the existing clinical biochemical detection index, and leukemia lesion prophase mRNA level screening can be really achieved. In addition, the detection method has characteristics of simpleness, convenience and low cost, and is applicable for promotion and application in county and district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

Various early-stage cancer pathology evolution MICRORNA-16 level in situ hybridization detection kit, detection method thereof and application thereof

The invention discloses an in situ hybridization detection kit which comprises a hybridization probe and a tag. The invention also discloses an in situ hybridization detection method for the microRNA-16 closely related with the various early-stage cancer pathology evolution through the kit. The method comprises the following steps: 1, contacting RNA to be detected in a substrate with the hybridization probe on condition that the hybridization probe and a target sequence can form a stable hybridization complex; and 2, detecting the hybridization complex. According to the kit and the detection method of the invention, the microRNA-16 expression level can be detected at the RNA level, and the abnormality of the microRNA-223 expression level is earlier than the abnormality of indexes detected with the imaging medicine and the present clinical biochemistry, so real early-stage canceration RNA level screening can be realized. The detection method which has the advantages of simplicity, convenience and low cost is convenient for the popularization and the application in county and district hospitals.
Owner:SUZHOU FUYING GENE TECH

In-situ hybridization assay kit for premalignant lung cancer 14-3-3thta level as well as assay method and application

The invention discloses an in-situ hybridization assay kit, comprising a hybridization probe and a marker. The invention also discloses a method for assaying mRNA of a 14-3-3thta gene closely related with the pathology evolution of premalignant lung cancer through in-situ hybridization by using the kit, and the method comprises the following steps: (1) contacting RNA to be assayed in a substrate with the hybridization probe to form a hybridization complex under a condition that the hybridization probe and a target sequence can form a stable hybridization complex; and (2) assaying the hybridization complex. The kit and the assay method, provided by the invention, can assay an expression level of the 14-3-3thta gene in an mRNA level, are earlier than medical imaging and the existing clinical biochemical assay indexes, and can really realize the screening of a premalignant mRNA level. In addition, the assay method of the invention is simple and convenient, is low in cost, and can be popularized and applied in county and district hospitals conveniently.
Owner:NATUREGEN BIOTECH SHANGHAI

Automatic grabbing, transporting and rescuing device based on heavy-load unmanned aerial vehicle

The invention discloses an automatic grabbing, transporting and rescuing device based on a heavy-load unmanned aerial vehicle. The device comprises a heavy-load unmanned aerial vehicle, a hoisting part, an automatic grabbing part, a multifunctional cabin and an equipment fixing part, the device can be used for emergency rescue, logistics transportation and the like. The problems of delayed rescue,air rescue equipment shortage, incapability of rescuing in dangerous and complex zones, low logistics transportation efficiency and the like in real life are solved through quick response of the unmanned aerial vehicle; along with the development of a unmanned aerial vehicle technology, the technology is increasingly mature, compared with a traditional helicopter, the unmanned aerial vehicle is lower in air rescue and transportation manufacturing cost; automatic flight, electric drive, and smart cruise can be achieved, the problem of pilot shortage can be solved; each firefighter is guaranteed to drive, the product can be equipped and used by each local unit, the emergency rescue capability of the local units can be greatly improved, the device can also be used for logistics transportation of natural disasters, epidemic areas and hospitals, timely support of materials is guaranteed, epidemic diffusion in the epidemic areas is avoided, timely support can be achieved, and manpower and financial resources can be saved.
Owner:CHANGCHUN NORMAL UNIVERSITY

In situ hybridization detection kit for early uterocarcinoma, detecting method and use

The invention relates to an in-situ hybridization detection reagent kit for early uterine cancer, which comprises a hybridization probe and a marker, wherein a sequence of the hybridization probe is shown in SEQ ID NO.1. The invention also provides an in-situ hybridization detection method for a P2RX7 gene. In addition, the invention also provides application of the reagent kit to preparation of a medicine for detecting a uterine cancer disease. The reagent kit has the advantages that the reagent kit has the characteristics of high sensitivity and strong specificity. The detection method has convenient and simple operation, and can be universally used and promoted in district hospitals and above.
Owner:台州和和生物科技有限公司

Cancer pathologic evolution early-stage Gankyrin gene mRNA level in-situ hybridization detection kit, detection method and applications thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the Gankyrin gene RNA expression change, which is closely related with the pathologic evolution in the early stage of cancer. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of Gankyrin gene in the RNA level, can detect the cancer in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of cancer, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

mRNA (Messenger Ribose Nucleic Acid) horizontal in-situ hybridization detection kit of PTEN and PHLPP1 at early stage of prostate canceration and detection method and application thereof

InactiveCN102559883AEarly treatmentTreatment implementationMicrobiological testing/measurementHybridization probeClinical biochemistry
The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and discloses a method for detecting mRNAs of a phosphatase and tension homologue gene (PTEN) and protein phosphatase 1 (PHLPP1) which are closely relevant to prostatic cancer early-stage pathologic evolution in situ in a hybridized way by using the kit. The method comprises the following steps of: (1) under the condition that a stable hybridization complex can be formed by the hybridization probe and a target sequence, contacting an RNA to be detected in a substrate with a hybridization probe to form a hybridized complex; and (2) detecting the hybridization complex. The kit and the detection method disclosed by the invention can be used for detecting the expression amounts of PTEN and PHLPP1 genes on the mRNA level, which is earlier than image medicine and the conventional clinical biochemistry detection index, and real horizontal sieving of the mRNA at the early stage of canceration can be realized; and meanwhile, the detection method is easy and convenient, has low cost, and is convenient to popularize and apply in district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

Reagent used for diagnosing early cancer

The invention relates to a reagent used for diagnosing early cancer. The reagent consists of a reagent A and a reagent B which are independent from each other, wherein the reagent A consists of mixed aqueous solution of alcoholic solution, glacial acetic acid solution and hydrogen dioxide solution; 5-60 percent glacial acetic acid, 5-60 percent alcohol and 1-35 percent hydrogen peroxide are contained in each liter of the mixed aqueous solution; the reagent B is guaiac wood ester or dianisidine; the reagent A and the reagent B are mixed into a mixed solvent before use; and the mixed solvent is dropped on a sample, and the color of the reagent is observed. The reagent is simple and easy to operate and can achieve quick detection. The reagent is of high value for early cancer screening, and is particularly easy to popularize in high-risk groups and areas revealed by statistical data. The reagent can achieve the same accurate detection effect in county or district hospitals and even township hospitals with less advanced medical equipment.
Owner:梁宏

Improved device for pine pile non-disturbance enclosure construction and construction method thereof

The invention belongs to an improved device for pine pile non-disturbance enclosure construction and a construction method thereof. The improved device comprises a vehicle body, an engine assembly, an operation room, a crawler belt running system, a first mechanical arm, a second mechanical arm, a first hydraulic cylinder, a second hydraulic cylinder and a third hydraulic cylinder, wherein the third hydraulic cylinder is arranged at the upper end of the second mechanical arm, a telescopic rod of the third hydraulic cylinder is connected with a grabbing and pressing device located at the front end of the second mechanical arm, and a drilling device is arranged at the lower end of the second mechanical arm. The method comprises the steps of device improvement, material preparation, construction lofting, pile planting construction which is divided into interval jumping type pile planting operation and the like. The improved device can work in the space close to historical protection buildings, residential areas and hospitals, disturbance to surface soil can be reduced, and the improved device has the advantages that the number of workers is small, the construction speed is high, and existing equipment can be quickly improved to be suitable for pine pile enclosure construction.
Owner:CHINA CONSTR MUNICIPAL ENG +1

Comprehensive detection kit for early carcinoma of prostate, detecting method and use thereof

The invention relates to a comprehensive detection reagent kit for early prostate cancer, which comprises two groups of hybridization probes and markers, wherein the hybridization probes have sequences shown in a sequence table SEQ ID NO.1 and a sequence table SEQ ID NO.2 respectively. The invention also provides an in-situ hybridization detection method for a PCADM-1 gene and a PSA gene, which comprises the following steps: a, the hybridization probes in the reagent kit contact RNA to be detected in a substrate to form a hybridization complex; and b, the hybridization complex obtained in step a is detected. The invention also provides application of the comprehensive detection reagent kit for the early prostate cancer to preparation of a medicine for comprehensively detecting an early prostate cancer disease. The reagent kit has the advantages that the reagent kit has the characteristics of high sensitivity and strong specificity. The detection method has convenient and simple operation, and can be universally used and promoted in district hospitals and above.
Owner:台州和和生物科技有限公司

In-situ hybridization assay kit for mRNA level of premalignant pancreatic cancer ATDC (telangiectasia group D associated protein) gene as well as assay method and application

The invention discloses an in-situ hybridization assay kit, comprising a hybridization probe and a marker. The invention also discloses a method for assaying mRNA of a telangiectasia group D associated protein (ATDC) gene closely related with the pathology evolution of premalignant pancreatic cancer through in-situ hybridization using the kit, and the method comprises the following steps: (1) contacting RNA to be assayed in a substrate with the hybridization probe to form a hybridization complex under a condition in which the hybridization probe and a target sequence can form a stable hybridization complex; and (2) assaying the hybridization complex. By use of the kit and the assay method of the invention, the expression level of the ATDC gene can be assayed in the mRNA level, the assay is earlier than medical imaging and traditional clinical biochemical detection indexes, and screening the premalignant mRNA level is really realized. In addition, the assay method of the invention is simple and convenient, is low in cost, and is convenient to be popularized and applied in county and district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

Solid tumor pathologic evolution early-stage GADD45G gene mRNA level in-situ hybridization detection kit, detection method and applications thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the GADD45G (growth arrest and DNA-damage-inducible, gamma) gene RNA expression change, which is closely related with the pathologic evolution in the early stage of solid tumor. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of GADD45G gene in the RNA level, can detect the cancer in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of canceration, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

In situ hybridization detection kit for PAX9 gene, and detecting method and use thereof

The invention relates to an in-situ hybridization detection reagent kit for a PAX9 gene, which comprises a hybridization probe and a marker, wherein a sequence of the hybridization probe is shown in SEQ ID NO.1. The invention also provides an in-situ hybridization detection method for the PAX9 gene. In addition, the invention also provides application of the reagent kit to preparation of a medicine for detecting a lung cancer disease. The reagent kit has the advantages that the reagent kit has the characteristics of high sensitivity and strong specificity. The detection method has convenient and simple operation, and can be universally used and promoted in district hospitals and above.
Owner:NATUREGEN BIOTECH SHANGHAI

Kit for mRNA level in-site hybridization detection in leukemia precursor lesion, and detecting method and application thereof

The invention discloses a kit for in-site hybridization detection, which comprises hybridization probes and markers. The invention further discloses a method for detecting mRNAs of transcription factor (DJ-1) genes closely related with pathologic evolution of leukemia precursor lesion by using the kit through in-site hybridization, wherein the method comprises the following steps of: (1), under the condition that the hybridization probes and target sequences can form a steady hybridization compound, RNAs to be detected in substrate are contacted with the hybridization probes so as to form the hybridization compound; and (2), the hybridization compound is detected. The kit and a detecting method disclosed by the invention can detect expression amount of the DJ-1 genes on a basis of the mRNA level, so that the prior clinical biochemical index detection is earlier, and mRNA level screening of the leukemia precursor lesion can be realized really. Meanwhile, the detecting method is simple and convenient, low in cost, and convenient for popularization and application in county and district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

In-situ hybridization assay kit for mRNA level of premalignant CD151 as well as assay method and application

The invention discloses an in-situ hybridization assay kit, comprising a hybridization probe and a marker. The invention also discloses a method for assaying mRNA of a tetraspanin (CD151) gene closely related with the premalignant pathology evolution through in-situ hybridization using the kit, and the method comprises the following steps: (1) contacting RNA to be assayed in a substrate with the hybridization probe to form a hybridization complex under a condition in which the hybridization probe and a target sequence can form a stable hybridization complex; and (2) assaying the hybridization complex. By using the kit and the assay method of the invention, the expression level of the CD151 gene can be assayed in the mRNA level, the assay is earlier than medical imaging and traditional clinical biochemical detection indexes, and screening the premalignant mRNA level is really realized. In addition, the assay method of the invention is simple and convenient, is low in cost, and is convenient to be popularized and applied in county and district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

mRNA (Messenger Ribose Nucleic Acid) horizontal in-situ hybridization detection kit of MSMB at early stage of prostate canceration and detection method and application thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and discloses a method for detecting the mRNA of a beta-microsemino-protein (MSMB) gene which is closely relevant to prostatic cancer early-stage pathologic evolution in situ in a hybridized way by using the kit. The method comprises the following steps of: (1) under the condition that a stable hybridization complex can be formed by the hybridization probe and a target sequence, contacting an RNA to be detected in a substrate with a hybridization probe to form a hybridized complex; and (2) detecting the hybridization complex. The kit and the detection method disclosed by the invention can be used for detecting the expression amount of the MSMB gene on the mRNA level, which is earlier than image medicine and the conventional clinical biochemistry detection index, and real horizontal sieving of the mRNA at the early stage of canceration can be realized; and meanwhile, the detection method is easy and convenient, has low cost, and is convenient to popularize and apply in district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

Cancer pathologic evolution early-stage GPR116 gene mRNA level in-situ hybridization detection kit, detection method and applications thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the GPR116 gene RNA expression change, which is closely related with the pathologic evolution in the early stage of cancer. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of GPR116 gene in the RNA level, can detect the cancer in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of cancer, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

In-situ hybridization detection kit for detecting mRNA (messenger ribonucleic acid) level in early stage of brain glioma tumor change and application, detection method and application

The invention discloses an in-situ hybridization detection kit comprising a hybridization probe and a marker, and also discloses a method using the kit to carry out in-situ hybridization detection on mRNA (messenger ribonucleic acid) of a palindrome gene (FUBP1) closely related to early stage pathology evolution of brain glioma. The method comprises the following steps of: (1) under the condition that the hybridization probe and a target sequence can form a stable hybridization compound, contacting the RNA to be detected in a substrate with the hybridization probe to form the hybridization complex; and (2) detecting the hybridization complex. The kit and the detection method disclosed by the invention can detect the expression quantity of gene in mRNA level, and can detect pathological changes earlier than image medicine and current biochemical detection indices, thus being capable of realizing real mRNA-level screening at the early stage of canceration; and meanwhile, the detection method disclosed by the invention is simple and convenient, low in cost and convenient to be popularized and applied in county and district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI
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