In situ hybridization detection kit for mRNA level at precancerous stage of breast cancer, detection method and application thereof

Abstract

The invention discloses an in situ hybridization detection kit, which comprises a hybridization probe and a marker. The invention further discloses a method for in situ hybridization detection of an mRNA level of a zinc finger protein 703 (ZNF703) gene closely related with pathological evolution at a precancerous stage of breast cancer by using the kit. The method comprises the following steps that: (1) under a condition that the hybridization probe and a target sequence can form a stable hybrid complex, the RNA requiring detection in the substrate contacts the hybridization probe to form a hybrid complex; and (2) the hybrid complex is detected. With the kit and the detection method of the present invention, the expression amount of the ZNF703 gene on the mRNA level can be detected, such that the provision time of the index is earlier than the provision time of the index by medical imaging or by existing clinical biochemical detection, the screening on the mRNA level at the precancerous stage can be achieved truly. In addition, the detection method of the present invention has characteristics of simpleness, convenience and low cost, and is applicable for popularization and application in county hospitals and district hospitals.

Description

technical field [0001] The invention relates to the field of biological detection, more specifically, relates to the relevant detection technology related to the mRNA expression change (pathological evolution process) of breast cancer premutation. Background technique [0002] According to the information provided by authoritative organizations at home and abroad, there are 2.6 million new cancer cases in my country every year, nearly 2.1 million deaths, and more than 7 million patients. Globally, there are 8 million new cancer patients every year, nearly 8 million deaths, and more than 8,400 patients. Ten thousand people, the number will double by 2020, this is a set of terrible figures. The cost of cancer diagnosis and treatment is getting higher and higher. The annual treatment cost of cancer patients is 200,000 yuan (poor areas may be higher, and developed areas may be much higher than 200,000 yuan), and there are more than 7 million patients. The annual cost is 1.4 trill...

AI Technical Summary

Problems solved by technology

[0003] In 2005, eight institutions including the US Institutes of Health, the Cancer Institute, and the Centers for Disease Control and Prevention made an annual report, reviewing the anti-cancer war launched in 1972. The report believed that human beings had failed in the anti-cancer war, and the conclusion was that cancer died The rate did not decrease, and it listed several factors that caused the failure of the anti-cancer war: 1. Tumor cell heterogeneity (polymorphism); 2. Tumor cell drug resistance; 3. Incomplete design of anti-cancer drugs (animals) unscientific model design), etc.

[0015] In view of the fact that the current clinical diagnosis of cancer (imaging medicine and biochemical indicators are all diagnosed after tumor formation) is a late diagnosis, the treatment is also a late treatment, leading to a treatment model that does not reduce the mortality rate

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