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Novel coronavirus detection kit

A detection kit, coronavirus technology, applied in measurement devices, immunoassays, instruments, etc., can solve problems such as lack of laboratories and specialized staff

Pending Publication Date: 2021-01-01
BEIJING JINWOFU BIOENGINEERING TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the current detection situation, nucleic acid detection tests have high requirements for the testing environment, operator experience and equipment, but some backward areas or other grassroots community hospitals lack laboratories with corresponding equipment and specialized staff

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Screening of labeled tracers

[0035] In this embodiment, colloidal gold and red latex microspheres are used as markers for comparison, wherein colloidal gold adopts colloidal gold (chloroauric acid: trisodium citrate=1:1.1) of about 40 nm, and red latex microspheres adopts a diameter of 300 nm. Left and right red latex microspheres. 1. Colloidal gold labeling process:

[0036] 1. Take 5ml colloidal gold and add 40μL 0.1M K 2 CO 3 Adjust the pH of colloidal gold to 7.0;

[0037] 2. Add 100 μg of novel coronavirus (2019-nCoV) monoclonal antibody 1 and let stand for 45 minutes;

[0038] 3. Centrifuge at 8000r / min for 15min and discard the supernatant;

[0039] 4. Redissolve the precipitate obtained by centrifugation with 5ml of gold standard working solution, and apply the reconstituted solution to the glass fiber membrane SB08. After drying overnight, it is used together with the nitrocellulose membrane coated with the novel coronavirus (2019-nCoV) monoclonal antib...

Embodiment 2

[0052] Example of product preparation

[0053] 1) Label the novel coronavirus (2019-nCoV) monoclonal antibody 1 with latex microspheres, and prepare the immune microsphere solution (latex microspheres-novel coronavirus (2019-nCoV) monoclonal antibody 1 complex).

[0054] 2) Dilute the immune microsphere solution (latex microsphere-novel coronavirus (2019-nCoV) monoclonal antibody 1 complex) appropriately and coat them on release pads respectively to prepare immune microsphere pads.

[0055] 3) Use the novel coronavirus (2019-nCoV) monoclonal antibody 2 to prepare the detection line (T line) solution, and use goat anti-mouse IgG to prepare the quality control line (C line) solution.

[0056] 4) Spray C and T lines on the nitrocellulose membrane to prepare an immune nitrocellulose membrane.

[0057] 5) Dry the immune microsphere pad and the immune nitrocellulose membrane.

[0058] 6) Use immune microsphere pad (coated latex microspheres-new coronavirus (2019-nCoV) monoclonal a...

Embodiment 3

[0063] Kit preparation process parameter screening

[0064] 1. Preparation process of immune microsphere solution

[0065] The steps of latex labeling were carried out in accordance with the relevant literature and manufacturer's instructions, and the principle of EDC activation labeling was adopted. The basic steps are as follows:

[0066] (1) Latex pretreatment: Measure the latex microsphere solution diluted to 1%, add the labeling buffer MES to the batch volume and wash, centrifuge at 13000rpm for 20 minutes, discard the supernatant; add MES to the batch volume to redissolve the precipitate, and sonicate Uniformly, centrifuge at 13000rpm for 20 minutes, discard the supernatant;

[0067] (2) EDC activation: the precipitation adds a certain volume of labeling buffer MES, and the EDC (0.01ml / ml) of a certain volume of 15mg / ml, the EDC addition volume and concentration are the addition volume ratio of 1% latex microsphere solution, avoiding volume The impact of changes on th...

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Abstract

The invention discloses a novel coronavirus detection kit. The novel coronavirus detection kit comprises antigen detection test paper. The antigen detection test paper comprises a lining, absorbent paper, an immune nitrocellulose membrane and an immune microsphere pad, wherein the immune microsphere pad, the immune nitrocellulose membrane and the absorbent paper are adhered to the lining. The immune microsphere pad is coated with a novel coronavirus 2019-nCoV monoclonal antibody 1 marked by latex microspheres; and the immune nitrocellulose membrane is provided with a detection line coated witha novel coronavirus 2019-nCoV monoclonal antibody 2 and a quality control line coated with a goat anti-mouse IgG polyclonal antibody. According to the invention, latex particles are used as a markingtracer, and a target object is detected and analyzed by using an antigen-antibody reaction and a lateral chromatography method. The kit has the advantages of convenience and quickness in actual operation, detection personnel do not need to detect professional knowledge, detection experiments do not need professional equipment configuration, and the kit can be widely used in primary community hospitals and health service centers.

Description

technical field [0001] The invention relates to a kit for detecting novel coronavirus. Background technique [0002] Since the end of 2019, the novel coronavirus pneumonia (2019-nCoV) caused by the infection of the novel coronavirus (2019-nCoV) has spread widely around the world, seriously affecting the health and economic development of all human beings, and has been listed by the World Health Organization as " public health emergency of international concern". [0003] At present, for the diagnosis of 2019-nCoV, nucleic acid detection based on polymerase chain reaction (PCR) reaction is mainly used, combined with clinical symptoms and epidemiological data of patients. According to the current detection situation, nucleic acid detection tests have high requirements for the testing environment, operator experience and equipment, but some backward areas or other grassroots community hospitals lack laboratories with corresponding equipment and specialized staff. Therefore, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543G01N33/577G01N33/532
CPCG01N33/56983G01N33/54313G01N33/558G01N33/577G01N33/532G01N2333/165G01N33/54388G01N2469/10G01N33/585
Inventor 陈浪王丽
Owner BEIJING JINWOFU BIOENGINEERING TECH CO LTD
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