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47results about How to "Implement early screening" patented technology

Messenger ribonucleic acid (mRNA) level in-situ hybridization detection kit for neoplasia early stage of intracranial glioma, and detection method and application

The invention discloses an in-situ hybridization detection kit which comprises a hybridization probe and a marker, and a method for performing n-situ hybridization detection on messenger ribonucleic acid (mRNA) of a circulating immune complex (CIC), which is closely related with the pathologic evolution of the early stage of intracranial glioma by using the kit. The method comprises the following steps of: 1) contacting RNA to be detected in substrate and a hybridization probe under the condition that the hybridization probe and a target sequence can form a stable hybridization complex to form the hybridization complex; and (2) detecting the hybridization complex. By the kit and the detection method, the gen expression level can be detected at the mRNA level; the obtained detection index is earlier than the detection indexes of medical imaging and the conventional clinical chemistry; real mRNA-level screening of the earlier stage of canceration can be realized; and meanwhile, the detection method is simple and convenient, and low in cost, and can be conveniently popularized and applied in county hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

Real-time fluorescence quantitative PCR detection kit for quantitative detection of GGPPS1 genes, detection method and application

The invention discloses a fluorescence quantitative PCR kit for detecting GGPPS1 in a liver tissue of a liver cancer sample. The kit comprises the following ingredients: a specific primer and a fluorescence quantitative PCR reaction solution. The invention further discloses a using method of the fluorescence quantitative PCR kit for detecting the GGPPS1 in the liver tissue. By utilizing the kit, the expression of the GGPPS1 in the liver tissue can be fast and quantitatively detected, and the detection result can be used as a diagnosis standard of clinical liver cancer in early stage or advanced stage. The kit disclosed by the invention has the advantages of simplicity and convenience in operation, high speed, stable result, high sensitivity and strong specificity.
Owner:NANJING UNIV

Messenger ribonucleic acid (mRNA) level in-situ hybridization detection kit for JAGGED1 in early stage of pathological change of breast cancer bone metastasis, and detection method and application

The invention discloses an in-situ hybridization detection kit which comprises a hybridization probe and a marker, and a method for performing in-situ hybridization detection on messenger ribonucleic acid (mRNA) of signal transducer protein (Jagged1), which is closely related with the pathologic evolution of the early stage of breast cancer bone metastasis by using the kit. The method comprises the following steps of: (1) contacting RNA to be detected in substrate and the hybridization probe under the condition that the hybridization probe and a target sequence can form a stable hybridization complex to form the hybridization complex; and (2) detecting the hybridization complex. By the kit and the detection method, the expression level of JAGGED1 gene can be detected at the mRNA level; a detection index is earlier than the detection indexes of medical imaging and the conventional clinical chemistry; real mRNA-level screening of the early stage of canceration can be realized; and meanwhile, the detection method is simple and convenient, and low in cost and can be conveniently popularized and applied in county hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

MRNA level in-situ hybridization detection kit for beta-catenin of liver cancer prophase as well as detection method and application of mRNA level in-situ hybridization detection kit

The invention discloses an in-situ hybridization detection kit. The in-situ hybridization detection kit comprises a hybridization detection kit probe and a marker. The invention further discloses an mRNA (Messenger Ribonucleic Acid) method of a signal channel (beta-catenin) closely related with pathologic evolution of liver cancer prophase by using in-situ hybridization of the kit. The mRNA method comprises the following steps: firstly, on the condition that the hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with the hybridization probe to form a hybridization complex; and secondly, detecting the hybridization complex. The kit and the detection method disclosed by the invention can be used for detecting the expression of the beta-catenin gene on the mRNA level; and compared with imaging medicine and a traditional clinical biochemical detection index, the kit and the detection method can be used for really realizing mRNA level screening of canceration prophase at earlier stage. Meanwhile, the detection method disclosed by the invention is simple and convenient and low in cost and is convenient to popularize and apply to county hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

MicroRNA-155 level in various types of cancerous lesions prophase in situ hybridization detection kit, detection method and application thereof

The invention discloses an in situ hybridization detection kit, which includes a hybridization probe and a marker. The invention also discloses an in situ hybridization detection method of microRNA-155 which is closely related to cancer prophase pathologic development by the use of the kit and comprises the following steps of: (1) on the condition that the hybridization probe and a target sequence can form a stable hybridization complex, making RNA to be detected in a substrate to contact with the hybridization probe to form the hybridization complex and (2) detecting the hybridization complex. The detection kit and the detection method provided by the invention can be used to detect the expression level of microRNA-155 gene on the level of mRNA, which is earlier than detection indexes of imaging medicine and present clinical biochemistry and can realize truly precancerous mRNA level screening. At the same time, the detection method provided by the invention is simple and convenient, requires low cost and is easy for popularization and application at country hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

MRNA-level in-situ hybridization detection kit for KLOTHO gene in pancreas precancerous period of KLOTHO gene in prophase of pancreas cancer detecting method and application

The invention discloses a in-situ hybridization detection kit, comprising a hybridization probe and a marker. The invention also discloses an mRNA-level method for in-situ hybridization detection of a Klotho gene which is closely related to early pathologic evolution of pancreatic cancers by using the kit which is closely related to the pathology evolution in the prophase of pancreas cancer, comprising the following steps of, (1) under the condition that the hybridization probe and a target sequence can form a steady hybrid complex, contacting the RNA to be detected in a substrate with the hybridization probe, to form a hybrid complex; (2) detecting the hybrid complex. The kit and the detecting method of the invention can detect the expression level of the Klotho gene at mRNA level and are earlier than medical imaging and present clinic biochemistry detecting indexes. The kit and the detecting method of the invention can realize real mRNA-level screening in precancerous periods, and meanwhile, the detecting method of the invention is simple and convenient and has low cost, which makes the method easy to be applied in county level hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

LET-7micro ribose nucleic acid (MIRNA) level in-situ hybridization detection kit for pathologic evolution early stage of various cancer, and detection method and application

The invention discloses an in-situ hybridization detection kit which comprises a hybridization probe and a marker, and a method for performing in-situ hybridization detection on let-7micro ribose nucleic acid (miRNA) which is closely related with the pathologic evolution of the early stage of various cancer by using the kit. The method comprises the following steps of: (1) contacting RNA to be detected in substrate and the hybridization probe under the condition that the hybridization probe and a target sequence can form a stable hybridization complex to form the hybridization complex; and (2) detecting the hybridization complex. By the kit and the detection method, the let-7miRNA expression level can be detected at the RNA level; and a detection index is earlier than the detection indexes of medical imaging and the conventional clinical chemistry; real RNA-level screening of the early stage of canceration can be realized; and meanwhile, the detection method is simple and convenient, and low in cost and can be conveniently popularized and applied in county hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

In-situ hybridization assay kit for premalignant lung cancer 14-3-3thta level as well as assay method and application

The invention discloses an in-situ hybridization assay kit, comprising a hybridization probe and a marker. The invention also discloses a method for assaying mRNA of a 14-3-3thta gene closely related with the pathology evolution of premalignant lung cancer through in-situ hybridization by using the kit, and the method comprises the following steps: (1) contacting RNA to be assayed in a substrate with the hybridization probe to form a hybridization complex under a condition that the hybridization probe and a target sequence can form a stable hybridization complex; and (2) assaying the hybridization complex. The kit and the assay method, provided by the invention, can assay an expression level of the 14-3-3thta gene in an mRNA level, are earlier than medical imaging and the existing clinical biochemical assay indexes, and can really realize the screening of a premalignant mRNA level. In addition, the assay method of the invention is simple and convenient, is low in cost, and can be popularized and applied in county and district hospitals conveniently.
Owner:NATUREGEN BIOTECH SHANGHAI

Kit for assaying MICRORNA-34 level in early stage of pathologic evolution of various cancers through in situ hybridization and assay method and application

The invention discloses an in situ hybridization assay kit. The kit comprises a hybridization probe and a label. The invention also discloses a method for assaying microRNA-34 closely related to early-stage pathologic evolution of various cancers through in situ hybridization by using the kit. The method comprises the following steps: (1) under the condition that the hybridization probe and a target sequence can form a stable hybrid complex, contacting the RNA to be assayed in a substrate with the hybridization probe to form the hybrid complex; and (2) assaying the hybrid complex. The kit and assay method disclosed by the invention have the following beneficial effects: the microRNA-34 expression can be assayed on the RNA level and is earlier than the medical imaging and existing clinical biochemical assay indexes, so that real RNA level screening in the early stage of cancerization can be realized; and meanwhile, the assay method is simple and convenient, has low cost and is convenient to popularize and apply in the county and district level hospitals.
Owner:SUZHOU FUYING GENE TECH

In-situ hybridization detection kit for MICRORNA (MICRO Ribonucleic Acid)-92A level at pathologic evolution early stage of colon cancer as well as microRNA-92A in-situ hybridization detection method and application of microRNA-92a to preparation of in-situ hybridization detection kit for colon cancer

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker. The invention also discloses a method for detecting microRNA (micro Ribonucleic Acid)-92a which is closely correlated with the pathologic evolution at an early stage of colon cancer by using in-situ hybridization through the in-situ hybridization detection kit. The method comprises the following steps of: firstly, under the condition that the hybridization probe and a target sequence can form a stable hybrid complex, contacting RNA to be detected in a substrate with the hybridization probe to form a hybrid complex; and secondly, detecting the hybrid complex. According to the in-situ hybridization detection kit and the detection method disclosed by the invention, the expression of microRNA-92a can be detected in the RNA level, which is earlier than that by imaging medicine and traditional clinical biochemical detection index and the real RNA level screening at the early stage of canceration can be realized; and meanwhile, the detection method disclosed by the invention is simple and convenient, is low in cost and is convenience for popularization and application to county and district level hospitals.
Owner:SUZHOU FUYING GENE TECH

Cancer pathologic evolution early-stage Gankyrin gene mRNA level in-situ hybridization detection kit, detection method and applications thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the Gankyrin gene RNA expression change, which is closely related with the pathologic evolution in the early stage of cancer. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of Gankyrin gene in the RNA level, can detect the cancer in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of cancer, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

In-situ hybridization detection kit of miR-378 in early stage of pathologic evolution of gastric cancer, detection method and application

The invention discloses an in-situ hybridization detection kit which includes a hybridization probe and a marker. The invention also discloses a method for detecting change of expression of a miR-378 gene, which is closely relative to pathologic evolution in an early stage of gastric cancer cancerization, through the kit in a manner of in-situ hybridization detection. The method includes following steps: (1) on the condition that the hybridization probe and a target sequence can form a stable hybridization complex, contacting a to-be-detected miR-378 in a substrate with the hybridization probe to form the hybridization complex; and (2) detecting the hybridization complex. By means of the kit and the method, an expression quantity of the miR-378 can be detected on a miRNA level. Compared with image medicine and a clinical biochemical detection index in the prior art, The kit and the method can be applied in an earlier stage and can achieve a true RNA-level screening operation during the early stage of cancerization. Meanwhile, the detection method is simple and convenient, is low in cost and is suitable for being popularized in hospitals in counties or districts.
Owner:嘉兴瑞康生物科技有限公司

In-situ hybridization detection kit for MICRORNA (MICRO Ribonucleic Acid)-29A level at pathologic evolution early stage of colon cancer as well as microRNA-29A in-situ hybridization detection method and application of microRNA-29a to preparation of in-situ hybridization detection kit for colon cancer

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker. The invention also discloses a method for detecting microRNA (micro Ribonucleic Acid)-29a which is closely correlated with the pathologic evolution at an early stage of colon cancer by using in-situ hybridization through the in-situ hybridization detection kit. The method comprises the following steps of: firstly, under the condition that the hybridization probe and a target sequence can form a stable hybrid complex, contacting RNA to be detected in a substrate with the hybridization probe to form a hybrid complex; and secondly, detecting the hybrid complex. According to the in-situ hybridization detection kit and the detection method disclosed by the invention, the expression of microRNA-29a can be detected in the RNA level, which is earlier than imaging medicine and traditional clinical biochemical detection index and the real RNA level screening at the early stage of canceration can be realized; and meanwhile, the detection method disclosed by the invention is simple and convenient, is low in cost and is convenience for popularization and application to county and district level hospitals.
Owner:SUZHOU FUYING GENE TECH

In-situ hybridization assay kit for mRNA level of premalignant pancreatic cancer ATDC (telangiectasia group D associated protein) gene as well as assay method and application

The invention discloses an in-situ hybridization assay kit, comprising a hybridization probe and a marker. The invention also discloses a method for assaying mRNA of a telangiectasia group D associated protein (ATDC) gene closely related with the pathology evolution of premalignant pancreatic cancer through in-situ hybridization using the kit, and the method comprises the following steps: (1) contacting RNA to be assayed in a substrate with the hybridization probe to form a hybridization complex under a condition in which the hybridization probe and a target sequence can form a stable hybridization complex; and (2) assaying the hybridization complex. By use of the kit and the assay method of the invention, the expression level of the ATDC gene can be assayed in the mRNA level, the assay is earlier than medical imaging and traditional clinical biochemical detection indexes, and screening the premalignant mRNA level is really realized. In addition, the assay method of the invention is simple and convenient, is low in cost, and is convenient to be popularized and applied in county and district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

Solid tumor pathologic evolution early-stage GADD45G gene mRNA level in-situ hybridization detection kit, detection method and applications thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the GADD45G (growth arrest and DNA-damage-inducible, gamma) gene RNA expression change, which is closely related with the pathologic evolution in the early stage of solid tumor. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of GADD45G gene in the RNA level, can detect the cancer in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of canceration, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

In-situ hybridization detection kit for MICRORNA (MICRO Ribonucleic Acid)-145 level at pathologic evolution early stages of various colon cancers as well as microRNA-145 in-situ hybridization detection method and application of microRNA-145 to preparation of in-situ hybridization detection kit for colon cancers

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker. The invention also discloses a method for detecting microRNA (micro Ribonucleic Acid)-145 which is closely correlated with the pathologic evolution at an early stage of colon cancer by using in-situ hybridization through the in-situ hybridization detection kit. The method comprises the following steps of: firstly, under the condition that the hybridization probe and a target sequence can form a stable hybrid complex, contacting RNA to be detected in a substrate with the hybridization probe to form a hybrid complex; and secondly, detecting the hybrid complex. According to the in-situ hybridization detection kit and the detection method disclosed by the invention, the expression of microRNA-145 can be detected in the RNA level, which is earlier than imaging medicine and traditional clinical biochemical detection index and the real RNA level screening at the early stage of canceration can be realized; and meanwhile, the detection method disclosed by the invention is simple and convenient, is low in cost and is convenience for popularization and application to county and district level hospitals.
Owner:SUZHOU FUYING GENE TECH

Kit and method for detecting horizontal in situ hybridization of MICRORNA-373 at the early pathological evolution stage of a variety of cancers and application thereof

The invention discloses a kit for detecting in situ hybridization, comprising a hybridization probe and a marker. The invention further discloses a method for detecting the in situ hybridization of MICRORNA-373 which is closely related to the early pathological evolution of a variety of cancers by using the kit. The method comprises the following steps: (1) making RNA to be detected in a substrate contact the hybridization probe to form a hybrid complex under the condition that the hybridization probe can form the stable hybrid complex together with a target sequence; and (2) detecting the hybrid complex. The kit and the method can be used for detecting the expression quantity of the MICRORNA-372 and the MICRORNA-373 on the RNA level earlier than the existing medical imaging technique and the existing clinical biochemical detection indicator and can realize the real screening of the RNA level at the earlier stage of canceration. The method is simple and convenient to operate, has low cost and can be popularized and applied to the county and district level hospitals.
Owner:SUZHOU FUYING GENE TECH

mRNA (Messenger Ribose Nucleic Acid) horizontal in-situ hybridization detection kit of PITX1 at early stage of canceration and detection method and application thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and discloses a method for detecting a telomerase inhibition (PITX1) gene which is closely relevant to prostatic cancer early-stage pathologic evolution, and an mRNA (Messenger Ribose Nucleic Acid) in situ in a hybridized way by using the kit. The method comprises the following steps of: (1) under the condition that a stable hybridization complex can be formed by a hybridization probe and a target sequence, contacting an RNA to be detected in a substrate with the hybridization probe to form a hybridized complex; and (2) detecting the hybridized complex. According to the kit and the detection method disclosed by the invention, the expression amount of the PITX1 gene can be detected on an mRNA level, which is earlier than image medicine and the conventional clinical biochemistry detection index, and real mRNA horizontal sieving at the early stage of canceration can be realized; and meanwhile, the detection method disclosed by the invention is easy and convenient, has low cost, and is convenient to popularize and apply in county hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

In-situ hybridization assay kit for mRNA level of premalignant CD151 as well as assay method and application

The invention discloses an in-situ hybridization assay kit, comprising a hybridization probe and a marker. The invention also discloses a method for assaying mRNA of a tetraspanin (CD151) gene closely related with the premalignant pathology evolution through in-situ hybridization using the kit, and the method comprises the following steps: (1) contacting RNA to be assayed in a substrate with the hybridization probe to form a hybridization complex under a condition in which the hybridization probe and a target sequence can form a stable hybridization complex; and (2) assaying the hybridization complex. By using the kit and the assay method of the invention, the expression level of the CD151 gene can be assayed in the mRNA level, the assay is earlier than medical imaging and traditional clinical biochemical detection indexes, and screening the premalignant mRNA level is really realized. In addition, the assay method of the invention is simple and convenient, is low in cost, and is convenient to be popularized and applied in county and district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

Cancer pathologic evolution early-stage GPR116 gene mRNA level in-situ hybridization detection kit, detection method and applications thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the GPR116 gene RNA expression change, which is closely related with the pathologic evolution in the early stage of cancer. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of GPR116 gene in the RNA level, can detect the cancer in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of cancer, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

Cancer metastasis pathologic evolution early-stage HOPX gene mRNA level in-situ hybridization detection kit, detection method and applications thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the HOPX gene RNA expression change, which is closely related with the pathologic evolution in the early stage of cancer metastasis. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of HOPX gene in the RNA level, can detect the cancer metastasis in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of cancer metastasis, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

HELQ gene mRNA level in-situ hybridization detection kit, detection method and applications thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the HELQ gene RNA expression change, which is closely related with the pathologic evolution in the early stage of ovarian cancer. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of HELQ gene in the RNA level, can detect the cancer in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of ovarian cancer, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

In-situ hybridization assay kit for mRNA level of premalignant CDK8 (cyclin-dependent kinase 8) as well as assay method and application

The invention discloses an in-situ hybridization assay kit, comprising a hybridization probe and a marker. The invention also discloses a method for assaying mRNA of a cyclin-dependent kinase 8 (CDK8) gene closely related with the pathology evolution of premalignant melanoma through in-situ hybridization using the kit, and the method comprises the following steps: (1) contacting RNA to be assayed in a substrate with the hybridization probe to form a hybridization complex under a condition in which the hybridization probe and a target sequence can form a stable hybridization complex; and (2) assaying the hybridization complex. By using the kit and the assay method of the invention, the expression level of the CDK8 gene can be assayed in the mRNA level, the assay is earlier than medical imaging and traditional clinical biochemical detection indexes, and screening the premalignant mRNA level is really realized. In addition, the assay method of the invention is simple and convenient, is low in cost, and is convenient to be popularized and applied in county and district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

In-situ hybridization detection kit for microRNA-182 level in early stage of pathological evolution of various cancers, and detection method and application thereof

The invention discloses an in-situ hybridization detection kit which comprises a hybridization probe and markers. The invention also discloses a method for applying the kit in in-situ hybridization detection of micro RNA-182 having a close relationship with pathological evolution of various cancers in an early stage, and the method comprises the following steps: (1) under the condition that the hybridization probe and a target sequence can form a stable hybrid complex, contacting RNA to be detected in a substrate with the hybridization probe so as to form a hybrid complex; (2) detecting the hybrid complex. The kit and the detection method provided in the invention can detect the expression level of micro RNA-182 at RNA level, enable detection indexes to be obtained at an earlier stage than medical imaging and conventional clinical biochemical detection do, and can realize genuine precancerous lesion RNA level screening; meanwhile, the detection method is simple and convenient, costs little and is convenient for popularization and application in county hospitals.
Owner:SUZHOU FUYING GENE TECH

Kit and method for detecting horizontal in situ hybridization of MICRORNA-372 at the early pathological evolution stage of a variety of cancers and application thereof

The invention discloses a kit for detecting in situ hybridization, comprising a hybridization probe and a marker. The invention further discloses a method for detecting the in situ hybridization of MICRORNA-372 which is closely related to the early pathological evolution of a variety of cancers by using the kit. The method comprises the following steps: (1) making RNA to be detected in a substrate contact the hybridization probe to form a hybrid complex under the condition that the hybridization probe can form the stable hybrid complex together with a target sequence; and (2) detecting the hybrid complex. The kit and the method can be used for detecting the expression quantity of the MICRORNA-372 on the RNA level earlier than the existing medical imaging technique and the existing clinical biochemical detection indicator and can realize the real screening of the RNA level at the earlier stage of canceration. The method is simple and convenient to operate, has low cost and can be popularized and applied to the county and district level hospitals.
Owner:SUZHOU FUYING GENE TECH

Messenger ribonucleic acid (mRNA) level in-situ hybridization detection kit for mH2A in early stage of canceration, and detection method and application

The invention discloses an in-situ hybridization detection kit which comprises a hybridization probe and a marker, and a method for performing in-situ hybridization detection on messenger ribonucleic acid (mRNA) of histone variant macro H2A (mH2A), which is closely related with the pathologic evolution of the early stage of black cancer by using the kit. The method comprises the following steps of: (1) contacting RNA to be detected in substrate and the hybridization probe under the condition that the hybridization probe and a target sequence can form a stable hybridization complex to form the hybridization complex; and (2) detecting the hybridization complex. By the kit and the detection method, the expression level of an mH2A gene can be detected at the mRNA level; a detection index is earlier than the detection indexes of medical imaging and the conventional clinical chemistry; real mRNA-level screening of the early stage of canceration can be realized; and meanwhile, the detection method is simple and convenient, and low in cost and can be conveniently popularized and applied in county hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

AGPS gene mRNA level in-situ hybridization detection kit, detection method and applications thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the AGPS (alkylglycerone phosphate synthase, AGPS) gene RNA expression change, which is closely related with the pathologic evolution in the early stage of canceration. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of AGPS gene in the RNA level, can detect the cancer in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of canceration, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI

In-situ hybridization detection kit for MICRORNA (MICRO Ribonucleic Acid)-233 level at pathologic evolution early stage of colon cancer as well as microRNA-233 in-situ hybridization detection method and application of microRNA-233 to preparation of in-situ hybridization detection kit for colon cancer

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker. The invention also discloses a method for detecting microRNA (micro Ribonucleic Acid)-233 which is closely correlated with the pathologic evolution at an early stage of colon cancer by using in-situ hybridization through the in-situ hybridization detection kit. The method comprises the following steps of: firstly, under the condition that the hybridization probe and a target sequence can form a stable hybrid complex, contacting RNA to be detected in a substrate with the hybridization probe to form a hybrid complex; and secondly, detecting the hybrid complex. According to the in-situ hybridization detection kit and the detection method disclosed by the invention, the expression of microRNA-233 can be detected in the RNA level, which is earlier than imaging medicine and traditional clinical biochemical detection index and the real RNA level screening at the early stage of canceration can be realized; and meanwhile, the detection method disclosed by the invention is simple and convenient, is low in cost and is convenience for popularization and application to county and district level hospitals.
Owner:SUZHOU FUYING GENE TECH

Horizontal in-situ hybridization detection kit and detection method as well as application for MICRORNA17-3P in earlier stage of cancer pathologic evolution

The invention discloses an in-situ hybridization detection kit which comprises a hybridization probe and a marker. The invention also discloses an in-situ hybridization detection method for microRNA-17-3p being closely related to the early pathologic evolution of various cancers by using the kit. The in-situ hybridization detection method comprises the following steps of: (1) under the condition that the hybridization probe and a target sequence can form a stable hybridization complex, leading RNA (Ribonucleic Acid) to be tested in a substrate to be in contact with the hybridization probe, and forming the hybridization complex; and (2) detecting the hybridization complex. The kit and the detection method provided by the invention can be used for detecting the expression quantity of the microRNA17-3p at the level of RNA, which is more early obtained in comparison with detection indexes of imaging medicine and the traditional clinical biochemistry, thereby the RNA level screening can be realized truly in the earlier stage of canceration, and simultaneously, the detection method provided by the invention is simple and convenient and low in cost and is convenient to popularize and apply in county-level hospitals.
Owner:SUZHOU FUYING GENE TECH

In-situ hybridization detection kit and detection method for liver cancer precancer NOTCH1 on mRNA (messenger ribonucleic acid) level, and applications thereof

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker. The invention also discloses a method for in-situ hybridization detection of mRNA (messenger ribonucleic acid) of signal conducting channel (Notch1) gene closely related to early-stage pathologic evolution of liver cancer by using the kit. The method comprises the following steps of: (1) contacting the RNA in a substrate to be detected with a hybridization probe under the condition that the hybridization probe and a target sequence can form a stable hybridization complex, so as to form the hybridization complex; and (2) detecting the hybridization complex. By adopting the kit and detection method, the expression amount of the NOTCH1 gene can be detected on the mRNA level, the detection time is earlier than that of imaging medicine and the traditional clinical biochemical detection indexes, and the mRNA-level screening and examination at the precancerous stage is truly realized; and at the same time, the detection method is simple, convenient, low in cost and convenient for popularization and application in county and district-level hospitals.
Owner:NATUREGEN BIOTECH SHANGHAI
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