The invention discloses an in-situ hybridization detection kit, which comprises a
hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the GADD45G (
growth arrest and
DNA-damage-inducible, gamma)
gene RNA expression change, which is closely related with the
pathologic evolution in the early stage of
solid tumor. The method comprises the following steps: (1) under a condition that a
hybridization probe and a target sequence can form a stable hybridization complex, contacting
RNA to be detected in a substrate with a
hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of GADD45G
gene in the
RNA level, can detect the
cancer in an earlier stage compared with the
medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of canceration, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.