47results about How to "Implement early screening" patented technology

The invention discloses a fluorescence quantitative PCR kit for detecting GGPPS1 in a liver tissue of a liver cancer sample. The kit comprises the following ingredients: a specific primer and a fluorescence quantitative PCR reaction solution. The invention further discloses a using method of the fluorescence quantitative PCR kit for detecting the GGPPS1 in the liver tissue. By utilizing the kit, the expression of the GGPPS1 in the liver tissue can be fast and quantitatively detected, and the detection result can be used as a diagnosis standard of clinical liver cancer in early stage or advanced stage. The kit disclosed by the invention has the advantages of simplicity and convenience in operation, high speed, stable result, high sensitivity and strong specificity.

The invention discloses an in-situ hybridization detection kit. The in-situ hybridization detection kit comprises a hybridization detection kit probe and a marker. The invention further discloses an mRNA (Messenger Ribonucleic Acid) method of a signal channel (beta-catenin) closely related with pathologic evolution of liver cancer prophase by using in-situ hybridization of the kit. The mRNA method comprises the following steps: firstly, on the condition that the hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with the hybridization probe to form a hybridization complex; and secondly, detecting the hybridization complex. The kit and the detection method disclosed by the invention can be used for detecting the expression of the beta-catenin gene on the mRNA level; and compared with imaging medicine and a traditional clinical biochemical detection index, the kit and the detection method can be used for really realizing mRNA level screening of canceration prophase at earlier stage. Meanwhile, the detection method disclosed by the invention is simple and convenient and low in cost and is convenient to popularize and apply to county hospitals.

The invention discloses an in situ hybridization detection kit, which includes a hybridization probe and a marker. The invention also discloses an in situ hybridization detection method of microRNA-155 which is closely related to cancer prophase pathologic development by the use of the kit and comprises the following steps of: (1) on the condition that the hybridization probe and a target sequence can form a stable hybridization complex, making RNA to be detected in a substrate to contact with the hybridization probe to form the hybridization complex and (2) detecting the hybridization complex. The detection kit and the detection method provided by the invention can be used to detect the expression level of microRNA-155 gene on the level of mRNA, which is earlier than detection indexes of imaging medicine and present clinical biochemistry and can realize truly precancerous mRNA level screening. At the same time, the detection method provided by the invention is simple and convenient, requires low cost and is easy for popularization and application at country hospitals.

The invention discloses an in-situ hybridization assay kit, comprising a hybridization probe and a marker. The invention also discloses a method for assaying mRNA of a 14-3-3thta gene closely related with the pathology evolution of premalignant lung cancer through in-situ hybridization by using the kit, and the method comprises the following steps: (1) contacting RNA to be assayed in a substrate with the hybridization probe to form a hybridization complex under a condition that the hybridization probe and a target sequence can form a stable hybridization complex; and (2) assaying the hybridization complex. The kit and the assay method, provided by the invention, can assay an expression level of the 14-3-3thta gene in an mRNA level, are earlier than medical imaging and the existing clinical biochemical assay indexes, and can really realize the screening of a premalignant mRNA level. In addition, the assay method of the invention is simple and convenient, is low in cost, and can be popularized and applied in county and district hospitals conveniently.

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the Gankyrin gene RNA expression change, which is closely related with the pathologic evolution in the early stage of cancer. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of Gankyrin gene in the RNA level, can detect the cancer in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of cancer, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker. The invention also discloses a method for detecting microRNA (micro Ribonucleic Acid)-145 which is closely correlated with the pathologic evolution at an early stage of colon cancer by using in-situ hybridization through the in-situ hybridization detection kit. The method comprises the following steps of: firstly, under the condition that the hybridization probe and a target sequence can form a stable hybrid complex, contacting RNA to be detected in a substrate with the hybridization probe to form a hybrid complex; and secondly, detecting the hybrid complex. According to the in-situ hybridization detection kit and the detection method disclosed by the invention, the expression of microRNA-145 can be detected in the RNA level, which is earlier than imaging medicine and traditional clinical biochemical detection index and the real RNA level screening at the early stage of canceration can be realized; and meanwhile, the detection method disclosed by the invention is simple and convenient, is low in cost and is convenience for popularization and application to county and district level hospitals.

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and discloses a method for detecting a telomerase inhibition (PITX1) gene which is closely relevant to prostatic cancer early-stage pathologic evolution, and an mRNA (Messenger Ribose Nucleic Acid) in situ in a hybridized way by using the kit. The method comprises the following steps of: (1) under the condition that a stable hybridization complex can be formed by a hybridization probe and a target sequence, contacting an RNA to be detected in a substrate with the hybridization probe to form a hybridized complex; and (2) detecting the hybridized complex. According to the kit and the detection method disclosed by the invention, the expression amount of the PITX1 gene can be detected on an mRNA level, which is earlier than image medicine and the conventional clinical biochemistry detection index, and real mRNA horizontal sieving at the early stage of canceration can be realized; and meanwhile, the detection method disclosed by the invention is easy and convenient, has low cost, and is convenient to popularize and apply in county hospitals.

The invention discloses an in-situ hybridization detection kit, which comprises a hybridization probe and a marker, and further discloses a method utilizing the in-situ hybridization detection kit to detect the HELQ gene RNA expression change, which is closely related with the pathologic evolution in the early stage of ovarian cancer. The method comprises the following steps: (1) under a condition that a hybridization probe and a target sequence can form a stable hybridization complex, contacting RNA to be detected in a substrate with a hybridization probe so as to form a hybridization complex; (2) detecting the obtained hybridization complex. The provided kit and detection method can detect the expression quantity of HELQ gene in the RNA level, can detect the cancer in an earlier stage compared with the medical imaging technology and conventional clinical biochemical index detection, can achieve real RNA level screening in the early stage of ovarian cancer, moreover, have the advantages of simpleness, convenience, and low cost, and thus are convenient to promote and use in county / district hospitals.

The invention discloses a kit for detecting in situ hybridization, comprising a hybridization probe and a marker. The invention further discloses a method for detecting the in situ hybridization of MICRORNA-372 which is closely related to the early pathological evolution of a variety of cancers by using the kit. The method comprises the following steps: (1) making RNA to be detected in a substrate contact the hybridization probe to form a hybrid complex under the condition that the hybridization probe can form the stable hybrid complex together with a target sequence; and (2) detecting the hybrid complex. The kit and the method can be used for detecting the expression quantity of the MICRORNA-372 on the RNA level earlier than the existing medical imaging technique and the existing clinical biochemical detection indicator and can realize the real screening of the RNA level at the earlier stage of canceration. The method is simple and convenient to operate, has low cost and can be popularized and applied to the county and district level hospitals.

The invention discloses an in-situ hybridization detection kit which comprises a hybridization probe and a marker, and a method for performing in-situ hybridization detection on messenger ribonucleic acid (mRNA) of histone variant macro H2A (mH2A), which is closely related with the pathologic evolution of the early stage of black cancer by using the kit. The method comprises the following steps of: (1) contacting RNA to be detected in substrate and the hybridization probe under the condition that the hybridization probe and a target sequence can form a stable hybridization complex to form the hybridization complex; and (2) detecting the hybridization complex. By the kit and the detection method, the expression level of an mH2A gene can be detected at the mRNA level; a detection index is earlier than the detection indexes of medical imaging and the conventional clinical chemistry; real mRNA-level screening of the early stage of canceration can be realized; and meanwhile, the detection method is simple and convenient, and low in cost and can be conveniently popularized and applied in county hospitals.