Kit and method for detecting horizontal in situ hybridization of MICRORNA-373 at the early pathological evolution stage of a variety of cancers and application thereof
A detection kit and detection method technology, applied in the relevant detection technology field, can solve the problems of non-decreased mortality, drug resistance of tumor cells, failure of the anti-cancer battle, etc., and achieve the effects of convenient operation, high sensitivity and strong specificity
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Embodiment 1
[0051] The in situ hybridization kit of this example was prepared according to conventional methods, and the kit included hybridization probes designed with MICRORNA-373, markers, and instructions, wherein: the probe marker of this example was digoxin.
[0052] Kit hybridization solution composition:
[0053] digestive juice 100μL / tube 1 tube / box colorless transparent liquid protective fluid 100μL / tube 1 tube / box colorless transparent liquid Pre-hybridization solution 1300μL / tube 2 tubes / box colorless transparent liquid Right-sense hybridization solution 10μL / tube 1 tube / box colorless transparent liquid antisense hybridization solution 10μL / tube 1 tube / box colorless transparent liquid blocking solution 1000μL / tube 1 tube / box colorless transparent liquid Alkaline phosphatase antibody 1 μL / tube 1 tube / box colorless transparent liquid Chromogen A 175μL / tube 1 tube / box yellow liquid Chr...
Embodiment 2
[0061] The implementation process of applying nucleic acid in situ hybridization detection method to the expression level of MICRORNA-373 in blood samples of each group:
[0062] 1).Take two specimens to be tested;
[0063] 2). Add 50 ml of digestive solution (100 μL of digestive solution plus 99.9 ml of 1× buffer Ⅰ, which is the concentration used) in a glass tank, preheat in a water bath at 37°C for 10 minutes, put 16 slides in, and treat at 37°C for 12 minutes , and then washed with 1× buffer I for 5 min;
[0064] 3). Use 0.2% protection solution (protection solution 1ml plus 1× buffer , 99ml is the concentration used), washed for 10 minutes, washed with three-distilled water for 5 minutes (the above process was carried out in a glass tank), took out the slide, and let it dry naturally;
[0065] 4). Put the slides into a humidifying box, add 25 μL / slice of pre-hybridization solution (add to the place where there are cells), cover with a cover glass, cover the humidifying...
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