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In situ hybridization detection kit for lung cancer , detecting method and use thereof

An in situ hybridization and comprehensive detection technology, which is applied in biochemical equipment and methods, and microbial determination/inspection, etc., can solve problems such as imperfect design of anticancer drugs, unreported detection methods, and drug resistance of tumor cells , to achieve the effect of radically curing malignant diseases of cancer, convenient operation and strong specificity

Inactive Publication Date: 2009-07-01
SUZHOU FUYING GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]In 2005, the American Institute of Health, the Cancer Institute, the Centers for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer" , that is to say, the cancer mortality rate has not been reduced, and it lists several factors that lead to the failure of the anti-cancer war: 1. Tumor cell heterogeneity; 2. Tumor cell drug resistance; 3. Incomplete design of anticancer drugs, etc.
However, there is no report about the comprehensive detection kit and detection method of lung cancer in situ hybridization

Method used

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  • In situ hybridization detection kit for lung cancer , detecting method and use thereof
  • In situ hybridization detection kit for lung cancer , detecting method and use thereof
  • In situ hybridization detection kit for lung cancer , detecting method and use thereof

Examples

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Effect test

Embodiment 1

[0051] A lung cancer in situ hybridization comprehensive detection kit, including four sets of hybridization probes and markers, the hybridization probes respectively have the sequence table SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.3 The sequence shown in ID NO.4. For the PAX9 gene sequence, see the sequence shown in SEQ ID NO.1. For the sequence of NKX2-8 gene, see the sequence shown in SEQ ID NO.2. See the sequence shown in SEQ ID NO.3 for the sequence of the TTF1 gene. For the sequence of RhoGDI2 gene, see the sequence shown in SEQ ID NO.4. Labeled with digoxin. The composition of other liquids and specimens in the kit is as follows:

[0052] Digestive solution 100μL / tube 4 tubes / box Colorless transparent liquid

[0053] Protective solution 100μL / tube 4 tubes / box Colorless transparent liquid

[0054] Pre-hybridization solution 1300μL / tube 8 tubes / box Colorless transparent liquid

[0055] Sense hybridization solution 10μL / tube 4 tubes / box Colorless transpar...

Embodiment 2

[0102]A kind of PAX9 gene, NKX2-8 gene, TTF1 gene and RHOGDI2 gene in situ hybridization detection method and kit application thereof

[0103] 1. Specimen processing

[0104] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0105] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0106] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0107] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowe...

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Abstract

The invention relates to an in-situ hybridization comprehensive detection reagent kit for lung cancer, which comprises four groups of hybridization probes and markers, wherein the hybridization probes have sequences shown in a sequence table SEQ ID NO.1, a sequence table SEQ ID NO.2, a sequence table SEQ ID NO.3, and a sequence table SEQ ID NO.4 respectively. The invention also provides an in-situ hybridization detection method for a PAX9 gene, a NKX2-8 gene, a TTF1 gene and a RHOGDI2 gene. In addition, the invention also provides application of the reagent kit to preparation of a medicine for detecting early diagnosis, metastasis and recurrence of the lung cancer. The reagent kit has the advantages that the reagent kit has the characteristics of high sensitivity and strong specificity. The detection method has convenient and simple operation, and can be universally used and promoted in district hospitals and above.

Description

【Technical field】 [0001] The invention relates to a kit, in particular to a lung cancer in situ hybridization comprehensive detection kit, detection method and application. 【Background technique】 [0002] According to the information provided by authoritative organizations at home and abroad, there are 1.7 million new cancer cases in my country every year, nearly 1.6 million deaths, and 6 million patients. In the world, there are 8 million new cancer patients every year, nearly 8 million deaths, and about 84 million patients. , by 2020 the number of people will double, which is a set of terrible figures. [0003] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors for the failure of the cancer war are: 1. Heterogeneity of tumor cells; 2. Drug re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张云福裘建英
Owner SUZHOU FUYING GENE TECH
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