Petroleum degradation bacterium in oil-containing sludge and application thereof
A technology of degrading bacteria and CNPC, applied in the field of microorganisms, can solve the problems of complex components of oily sludge, less research on microbial degradation and remediation, and difficult to degrade
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[0018] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.
[0019] This application uses the following methods to screen and confirm the type of bacterial strain, and at the same time provide various excellent properties of the bacterial strain, the specific content is as follows:
[0020] 1 Materials and methods
[0021] 1.1 Oily sludge
[0022] The oily sludge samples come from Bohai Oilfield drilling cuttings, which are taken from the Environmental Protection Technology Service Branch of CNOOC Bohai Company. The water content of the sludge is determined to be 70.55%, the pH value is 6.4, and the salinity is 3.0%.
[0023] 1.2 Medium
[0024] 1.2.1 Enrichment and separation medium (g / L) Yeast powder 3, NaCl5, MgSO 4 ·7H 2 O0.25, (NH 4 ) 2 SO 4 1,K 2 HPO 4 10, KH 2 PO 4 4. pH7.2~7.4.
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