Petroleum degradation bacterium in oil-containing sludge and application thereof

A technology of degrading bacteria and CNPC, applied in the field of microorganisms, can solve the problems of complex components of oily sludge, less research on microbial degradation and remediation, and difficult to degrade

Active Publication Date: 2014-09-03
TIANJIN UNIV OF SCI & TECH
View PDF1 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition of oily sludge, which is difficult to degrade, there are relatively few researches on microbial degradation and remediation of oily sludge at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Petroleum degradation bacterium in oil-containing sludge and application thereof
  • Petroleum degradation bacterium in oil-containing sludge and application thereof
  • Petroleum degradation bacterium in oil-containing sludge and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0019] This application uses the following methods to screen and confirm the type of bacterial strain, and at the same time provide various excellent properties of the bacterial strain, the specific content is as follows:

[0020] 1 Materials and methods

[0021] 1.1 Oily sludge

[0022] The oily sludge samples come from Bohai Oilfield drilling cuttings, which are taken from the Environmental Protection Technology Service Branch of CNOOC Bohai Company. The water content of the sludge is determined to be 70.55%, the pH value is 6.4, and the salinity is 3.0%.

[0023] 1.2 Medium

[0024] 1.2.1 Enrichment and separation medium (g / L) Yeast powder 3, NaCl5, MgSO 4 ·7H 2 O0.25, (NH 4 ) 2 SO 4 1,K 2 HPO 4 10, KH 2 PO 4 4. pH7.2~7.4.

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a petroleum degradation bacterium in oil-containing sludge. The degradation bacterium is preserved in the Chinese Academy of Sciences of the China General Microbiological Culture Collection Center CGMCC in #3, No.1 Courtyard, Western Beichen Road, Chaoyang District, Beijing, the preservation serial number is CGMCC8647, and the class number is Corynebacteriumglutamicum. The petroleum degradation bacterium (RSI) is separated from the oil-containing sludge in the Bohai Oilfield, the preservation serial number is CGMCC8647, the experiment shows that the RSI is relatively high in degradation rate for saturated hydrocarbon, that is, as high as 20.74 percent, the degradation rate of the bacterium for total petroleum hydrocarbon (TPH) in the oil-containing sludge within 30 days is 39.69 percent, and the optimal degradation conditions of the bacterium for petroleum are that the temperature is 37 DEG C, the salinity is 0.5-5 percent, and the pH value is 6-8.

Description

technical field [0001] The invention belongs to the field of microbes, and relates to a bacterial strain for degrading petroleum hydrocarbons, in particular to a petroleum-degrading bacterium in oily sludge and its application. Background technique [0002] Petroleum is one of the essential energy sources in today's social life and production, and it is also recognized as one of the pollutants that have the most serious impact on the environment. A large amount of oily sludge will be generated during the exploration, development, processing and transportation of petroleum. Oily sludge usually contains alkanes, aromatic hydrocarbons, non-hydrocarbon compounds, and asphaltenes, which can pollute the ecological environment through soil and groundwater and threaten human health. Therefore, the safe disposal of oily sludge is a major issue. The treatment and disposal of oily sludge mainly involves the reduction of oil content and the separation of water and sludge. The current ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/15C02F103/10C02F101/32
Inventor 刘宪斌任丽君田胜艳赵兴贵
Owner TIANJIN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap