Detection method for total petroleum hydrocarbon in contaminated soil

A technology of total petroleum hydrocarbons and detection methods, applied in the preparation of test samples, color/spectral characteristic measurement, etc., can solve the problems of long processing cycle, large solvent consumption, unfavorable popularization and application, etc., and reduce environmental hazards , The extraction process is simple, and the effect of high environmental friendliness

Inactive Publication Date: 2016-10-12
NANCHANG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional oil extraction methods usually use organic reagents such as acetone, dichloromethane, and carbon tetrachloride to extract oil from polluted soils. The processing cycle is long, the solvent consumption is large, and secondary pollution is likely to occur, which is not conducive to the friendly development of the environment; The cost of instruments and technologies such as microwave extraction and supercritical fluid extraction is relatively high, which is not conducive to popularization and application

Method used

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  • Detection method for total petroleum hydrocarbon in contaminated soil
  • Detection method for total petroleum hydrocarbon in contaminated soil
  • Detection method for total petroleum hydrocarbon in contaminated soil

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Determination of detection wavelength. Carry out detection wavelength scanning on petroleum solution (prepared by crude oil and extractant), the wavelength range is 200nm-400nm, the detection results are shown in figure 1 . Depend on figure 1 It can be seen that when the wavelength is between 300nm and 310nm, the absorbance of the petroleum solution is relatively high, and when the wavelength is 304nm, the absorbance is the highest, so the oil detection wavelength is preferably 304nm.

Embodiment 2

[0036] How to draw the standard curve. First prepare 5.0g / L petroleum solution (crude oil and petroleum ether preparation) as the stock solution, then accurately draw 0mL, 0.5mL, 1.0mL, 1.5mL, 2.0mL petroleum stock solution into a 50mL volumetric flask, and then fill it up to 2mL, and then dilute to 50mL with absolute ethanol. A series of standard solutions of 0 mg / L, 50 mg / L, 100 mg / L, 150 mg / L, and 200 mg / L were prepared, and then measured with a microplate reader at a wavelength of 304 nm. Draw the standard curve with the mass concentration (X) of the standard sample as the abscissa, and the corresponding absorbance value (y) as the ordinate, and the regression equation of the standard curve is y=0.0014x-0.012, and the correlation coefficient R 2 >0.9990, the linear relationship is good, see the results figure 2 .

Embodiment 3

[0038] Effect of different extraction reagents. Accurately weigh 2.00 g of soil with an oil pollution concentration of 2.0 g / kg and add it to a 50 mL Teflon centrifuge tube, respectively use petroleum ether, n-hexane, acetone and carbon tetrachloride as extraction reagents, add 8 mL of extraction reagents, each The reagent was repeated 4 times, with a total of 16 samples, placed in an ultrasonic cleaner for ultrasonic extraction for 30 minutes, and then centrifuged at 4000r / min for 10 minutes, and the supernatant was poured into a colorimetric tube; repeat the above steps to further extract the residue in the soil oil. Finally, the supernatants from the two extractions were combined, and the volume was adjusted to 50 mL with absolute ethanol. Then use microplate reader to carry out quantitative detection under 304nm wavelength, and utilize standard curve to carry out calculation and analysis, draw the efficiency that different extraction agents extract oil, see image 3 . D...

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Abstract

The invention relates to the technical field of determination of content of an organic mixture in soil and specifically discloses a detection method for total petroleum hydrocarbon in contaminated soil. The detection method comprises the following steps: 1) extracting: mixing to-be-detected soil with an extracting reagent, performing ultrasonic extraction, centrifuging and taking supernate as a to-be-detected sample solution, wherein petroleum ether is taken as the extracting reagent, the ratio of soil to liquid is 1:4, the ultrasonic extraction is performed for two times, the ultrasonic extraction duration is 20min each time and the ultrasonic extraction power is 100W; 2) determining: using a microplate reader for determining the absorbance value of the to-be-detected sample solution under the detection wavelength and utilizing an external standard method to determine the content of total petroleum hydrocarbon in the to-be-detected sample solution, wherein the detection wavelength is 304nm. The detection method provided by the invention has the advantages of less extracting agent dosage, low pollution, high detection speed, high accuracy, less determining solution dosage and suitability for quantitative determination of a trace of petroleum pollution environmental samples.

Description

technical field [0001] The invention relates to the technical field of measuring the content of organic matter mixture in soil. Background technique [0002] In terms of composition, petroleum is a complex mixture containing various hydrocarbons (normal alkanes, branched alkanes, aromatics, alicyclic hydrocarbons, etc.) and a small amount of other organic compounds (sulfides, nitrides, naphthenic acids, etc.). With the rapid development of the economy, oil leakage during oil extraction and transportation and the increasing use of its products have led to serious soil oil pollution problems. After the oil pollutes the soil, the basic physical and chemical properties of the soil change, and the oil is continuously adsorbed and retained in the soil. It will not only cause a large number of microorganisms in the soil to die, but also affect the normal growth of plants in it. In particular, polycyclic aromatic hydrocarbons in the soil are strong carcinogens, which can enter the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N1/34
CPCG01N21/33G01N1/34
Inventor 周际海黄荣霞濮海燕樊后保袁颖红李威刘文飞段洪浪
Owner NANCHANG INST OF TECH
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