159 results about "Manno-oligosaccharide" patented technology

The invention relates to a yeast cell wall, comprising 20%-70% by mass of beta-yeast glucan and 20%-70% by mass of mannan oligosaccharide. The invention also relates to a forage additive, comprising the yeast cell wall, sodium modified montmorillonite and selenium yeast, and by mass, a ratio of the yeast cell wall to the sodium modified montmorillonite is 1 : 9-9 : 1; and a ratio of a total of the yeast cell wall and the sodium modified montmorillonite to the selenium yeast is 10 : 1-20 : 1. The invention also relates to a preparation method for the yeast cell wall. The preparation method comprises the steps of breaking the cell wall of live yeast through self-dissolution, degrading the yeast with a compound enzyme, separating, purifying, drying and deactivating the enzyme to obtain the yeast cell wall, wherein the compound enzyme comprises papain, nuclease and bacteria protease, and by mass, the addition amount of the papain, nuclease and bacteria protease is 0.1-0.3%, 0.05-0.2% and 0.01-0.03% that of the dry material of the yeast. The invention also relates to forage, an addition level of the additive in the forage is 1.5-2.5 g/kg.

The invention discloses growth promoting fodder special for growing and fattening pigs. The growth promoting fodder is prepared from corn, wheat bran, sweet potato vine powder, cottonseed meal, double-low rapeseed meal, fish meal, shell powder, talcum powder, calcium hydrogen phosphate, salt, multivitamin for the pigs, methionine, cystine, seaweed meal, beta vulgaris pulp, mannan oligosaccharides, nettle meal, luceme meal, carrot meal, purslane meal, peanut vine meal, pine needle meal, a composite additive, complex enzyme preparation and microecologics. The composite additive is prepared from polygonum multiflorum, eucommia, malt, gynostemma pentaphyllum, medicated leaven, poria cocos, hawthorns, lycium, Chinese yam, methionine copper, ferrous sulfate, tryptophan zinc, tryptophan manganese, calcium iodate and sodium selenite. The growth promoting fodder special for the growing and fattening pigs is reasonable in formula, comprehensive in nutrition, good in palatability, and capable of promoting growth of the growing and fattening pigs and improving quality of pork.

The present invention discloses a food attractant growth promoting agent for aquatic animals and its manufacturing technology, its main components comprise: free amino acids, beta-dextran, mannan-oligosaccharide and nucleotide, its producing steps as follow: 1, debittering treatment for beer yeast; 2, centrifuging treatment; 3, filling compound enzyme into separating liquid; 4, processing the thermal insulating action; 5, increasing temperature till 90 DEG C to insulate heat continuously; 6, filling carries; 7, drying treatment. The merits of food attractant growth promoting agent of the present invention is: 1, adopting advanced biotechnology, it is natural, without poisonous and side effects; 2, enriching various amino acids, vitamins and minerals, it can be used and has nutrition function; 3, containing beta-dextran, mannan-oligosaccharide, small peptide and nucleotide etc. it can promote immunity of aquatic animals; 4, free amino acids, small peptide and nucleotide has food attracting function, increasing feed intake for aquatic animals, promoting growth.

The invention discloses low-protein daily ration for a growing pig under a net energy system and an application thereof, and belongs to the field of feed processing. The method comprises the steps of: taking the growing pig with the weight being 20-50kg as a research object, utilizing corn-bean pulp daily ration, reducing the protein content of the daily ration by 2% on the basis of the standard recommended by the National Research Council, simultaneously supplying lysine, methionine, threonine and tryptophan; adding mannan oligosaccharide, multivitamins, complex phosphoesterasum, probiotics and the like to form the low-protein daily ration for the growing pig under the net energy system; crushing, mixing, pelletizing then dosing, and packaging the pelletized feed and a probiotic component in a mixing manner to obtain the product disclosed by the invention. The animal feed utilization rate can be improved; the use quantity of antibiotic medicines in the feeding of animals is effectively reduced; the immunity of fed animals is improved; the security of animal meat products is improved; and the raise efficiency is also improved. The low-protein daily ration is scientific in process design, high in production efficiency, low in cost, and suitable for multi-variety and small-scale production.

The invention relates to beta-mannase mRmMan5A and an encoding gene and application thereof .Compared with original beta-mannase mRmMan5A, the beta-mannase mRmMan5A has the advantages that the optimal pH is low and is 4.5, and the optimal temperature is high and is 65 DEG C .Compared with beta-mannase reported in the past, the beta-mannase mRmMan5A has higher application value in the industries of food, feed and the like .The enzyme activity of beta-mannase of engineering bacteria fermentation liquor prepared by introducing the encoding gene of the protein into pichia pastoris can reach 72626 U/mL (protein content is 9.1 mg/mL), and efficient expression is achieved .The beta-mannase is used for hydrolyzing palm meal subjected to steam explosion, hydrolysate mainly contains mannan oligosaccharide with polymerization degree of 2 to 4, the mannan oligosaccharide yield is 19.6%, and the mannan oligosaccharide conversion rate is about 60%.

The invention provides reorganized mannase, genetically-engineered bacteria of the recombined mannose and a hydrolyzing preparation mannan oligosaccharide method. The hydrolyzing preparation mannan oligosaccharide method comprises the steps 1), optimizing and reorganizing beta-mannase genes according to pichia pastoris codon preference; 2), constructing expression vector pHBM905BDM-Man containing the beta-mannase genes, converting pitchia pastoris GS115 competence cells, and fostering and checking to obtain single-copy genetically-engineered bacteria; 3) constructing, fostering and checking to obtain multi-copy genetically-engineered bacterium; 4) using different-copy-number genetically-engineered bacteria to efficiently express and prepare reorganized beta-mannase; and 5) using reorganized beta-mannase hydrolysis mannan substrate to produce mannan oligosaccharide. Substrate hydrolysis concentration is 15-40%, temperature is 50 DEG C to 55 DEG C, and time is 0.5 hour to 2 hours. At present, two-copy beta-mannase genetically-engineered bacteria GS115/MAN78(CCTCCNo:2012554) is high in expression level. The activity of the beta-mannase is 5000U/mL (a decimal number system method), hydrolysis substrate specificity is high, and hydrolysis substrate concentration is obviously higher than that of other methods.

The invention discloses a compound synbiotics preparation and its application. The compound synbiotics preparation includes composite Probiotics and Prebiotics, the composite Probiotics comprise Bacillus subtilis and Bacillus licheniformis, the Prebiotics are anyone of yeast cell wall polysaccharides, mannan oligosaccharides, fructo-oligosaccharides, soybean oligosaccharides, isomalto oligosaccharides, lactosucrose, galactooligosaccharides, sucrose thermal oligosaccharides caramel and oligonucleotides xylose; and the survival rate of spores is higher than 90% after the mixing granulation of the compound synbiotics preparation and a feed, and the total number of live bacteria is 1.0*10<9>-1.0*10<11>CFU/g. By utilizing the cooperative effect of different Probiotics and Prebiotics, the compound synbiotics preparation avoids the disadvantages of single Probiotics, increases the content of the Probiotics, reduces the competitor rate, improves the heat resistance and the storability of the synbiotics, improves the quality of feeding synbiotics, and has a wide application prospect.

The present invention relates to a production method for preparing konjak mannoligose by using cellulase. Its technological process includes the following several procedures: enzymolysis, filtraction, decoloring, concentration and drying. It is characterized by that said invention adopts non-specific commercial enzyme (including cellulose and hemicellulase), and utilizes centrifugal filtering and membrane filtering combined process, so that said invention can obtain high-purity konjak mannoligose. Besides, said invention also provides the concrete requirements and steps of the above-mentioned every procedure.

The invention provides a production method of a health instant noodle food which has a regulating effect on intestinal and gastric microecology and is characterized in adding the health instant noodle food with functional oligosaccharides which comprise xylo-oligosaccharide, galacto-oligosaccharide, manno-oligosaccharide, stachyose, raffinose, fructo-oligosaccharide, isomalto-oligosaccharide, malto-oligosaccharide, soybean oligosaccharide, chitosan, lactosucrose, gentio-oligosaccharide; the mixture comprising one or more of the oligosaccharides constitutes the functional oligosaccharides and the functional oligosaccharides of 0.02-2.00 percent by weight of flour are mixed evenly with flour and processed in the workmanship of normal instant noodle food. The functional oligosaccharides pertain to bifidus factors; therefore, the invention has the physiological function of regulating the intestinal and gastric microecology of body to relieve constipation and lowering cholesterol and blood-sugar level, thereby providing a popular health food which has the functions of preventing and curing diseases.

The invention discloses a functional fruit and vegetable ferment solid beverage and a preparation method thereof. The beverage consists of the following main raw materials in parts by weight: 20-60 parts of maltodextrin, 10-40 parts of fruit and vegetable ferment powder, 1-30 parts of fruit juice powder, 0.5-10 parts of fructo-oligosaccharide, 0.5-10 parts of manno-oligosaccharide, 0.5-10 parts ofxylo-oligosaccharide, 0.5-10 parts of hemp seed extract, 0.5-10 parts of oriental waterplantain rhizome extract, 0.5-10 parts of cassia seed extract, 0.5-10 parts of bifidobacterium adolescentis, 0.5-8 parts of lactobacillus rhamnosus, 0.5-8 parts of lactobacillus plantarum, 0.1-0.8 part of edible essence and 0.005-0.05 part of sucralose. The beverage combines probiotics, prebiotics, fruit and vegetable ferment and traditional Chinese medicines, and can effectively relieve and treat constipation and prevent the recurrence of the constipation; also has effects in regulating intestinal flora balance, inhibiting generation of harmful bacteria and toxins and promoting reproduction and growth of beneficial bacteria; can regulate and restore gastrointestinal tract function, and enhance gastrointestinal motility; can promote the discharge of feces and toxins in intestinal tract; and can also promote digestion, promote metabolism, and improve body function.

The invention discloses a preparation method of galacto-mannan-oligosaccharides and application of the galacto-mannan-oligosaccharides. The preparation method comprises the following steps: (1) performing segmented composite enzymatic hydrolysis on galacto-mannan-oligosaccharides such as guar gum, sesbania gum, locust bean gum, fenugreek gum and Tara gum, wherein the enzyme is a complex enzyme ofacid mannase and cellulase; and (2) by segmented ultrafiltration classification, obtaining degraded galacto-mannan-oligosaccharides which are a mixture of a disaccharide, a trisaccharide, a tetrasccharide and a pentosaccharide. The preparation process is simple and easy, and is environmentally friendly. The conversion rate of the galacto-mannan-oligosaccharides is high, and the galacto-mannan-oligosaccharides are easy to separate. The prepared galacto-mannan-oligosaccharides can be used as prebiotics to be applied to food and animal feed, the proliferation of a butyric acid-production probiotic is promoted, and the intestinal environment is improved.

The invention relates to a nano nutritious supplementary with a function of fattening pigs and a preparation method thereof. The nano nutritious supplementary comprises the following components in parts by weight: 0.5-33 parts of vitamin A, 0.1-17 parts of vitamin D3, 0.6-24 parts of vitamin E, 0.1-12 parts of vitamin B1, 0.5-8 parts of vitamin B2 sodium phosphate, 0.01-1 part of vitamin B12, 0.2-13 parts of nicotinamide, 0.5-4 parts of calcium pantothenate, 0.1-8 parts of biotin, 0.4-2 parts of folic acid, 0.2-19 parts of vitamin C, 1-17 parts of glutamine, 0.2-4 parts of methionine, 0.6-7 parts of lysine, 0.1-3.9 parts of arginine, 0.8-6 parts of threonine, 2-25 parts of glycine, 10-70 parts of glucose, 1-10 parts of mannan oligosaccharide and 600-850 parts of distilled water. The nano nutritious supplementary has the functions of fattening pigs, and improving the stress resistance and the disease resistance of pigs.

The invention discloses a double-target anti-HIV (anti-human immunodeficiency virus) glycopeptide compound and an application thereof. The anti-HIV glycopeptide compound is a T20 compound modified with mannan oligosaccharide or oligosaccharide containing fucose at the terminal. The fixed-point glycosylated modified compound of an anti-HIV drug represented as M1-T20, M3-T20, M5-T20 or Lex-T20 is a double-target drug, can prolong the plasma half-life of the drug in the animals and enhance the HIV inhibiting effect, and has the great application value.

The invention provides a preparation method of a high-mannan-oligosaccharide palm meal fermented feed. The preparation method comprises the following steps of mixing an enzyme preparation with fermentation strains, palm meal and water, and performing anaerobic fermentation so as to obtain fermented materials. The preparation method disclosed by the invention is simple to operate, and the preparedpalm meal fermented feed is compounded with various active polysaccharides, small peptides in different molecular weight distributions and a large quantity of probiotics, wherein the content of mannanoligosaccharides reaches 18-25%, the content of the small peptides reaches 16-25%, the content of lactic acid is 2.5-3.5%, and the content of the probiotics is 1.0 * 10<8>cfu/g. The high-mannan-oligosaccharide palm meal fermented feed can promote ingestion of animals, can effectively enhance the immunity of the animals, can guarantee the health of intestinal tracts and can improve the productionperformance.

The invention relates to efficient environment-friendly silurus meridionalis growing period flotation puffing mixed feed and a manufacturing method thereof, and belongs to the technical field of aquaculture feed. The feed comprises fish meal, meat meal, fermented soybean meal, soybean meal, corn gluten meal, flour, soybean oil, monocalcium phosphate, 98.5-percent lysine hydrochloride, mannan oligosaccharide, sodium butyrate, 35-percent vitamin C acetate, antioxidants, vitamin premix and mineral substance premix. Silurus meridionalis is bred through the silurus meridionalis growing period flotation puffing mixed feed, compared with frozen fresh fishes and animal giblets, the nutrition is balancer, the feed is easier to store, use is convenient, and sound development of the silurus meridionalis culture industry can be promoted.

The invention relates to environment-friendly compound feed for reservoir ctenopharyngodon idellus and belongs to the technical field of fish feed. The feed comprises 10-15 parts of flour, 2-5 parts of fish meal, 5-10 parts of peanut meal, 3-8 parts of coconut meal, 3-8 parts of rubber seed meal, 3-10 parts of apple seed meal, 15-25 parts of rapeseed meal, 5-10 parts of citric acid waste, 20-25 parts of soybean meal, 1.5-3 parts of monocalcium phosphate, 0.3-0.5 part of decavitamin premix, 0.5-1 part of compound trace element premix, 0.03-0.1 part of mildew preventive, 0.02-0.1 part of glucan, 0.01-0.1 part of mannan oligosaccharide, 0.02-0.1 part of L-malic acid, 0.05-0.1 part of sodium butyrate and 0.05-0.1 part of bile acid. The feed can well promote the healthy development of the gut and thickening of the intestinal mucosa, reduce the adhesion hyperplasia of harmful germs on the gut, improve digestibility, reduce the occurrence of gut diseases, reduce the loss of nutrients and reduce pollution to water.

The invention relates to an anti-diarrhea feed additive for piglets. The anti-diarrhea feed additive for the piglets comprises the following ingredients: 30-50 parts of a plant essence oil mixture, 10-30 parts of a traditional Chinese medicine named herba portulacae, 10-25 parts of fructo-oligosaccharides, 10-25 parts of manno-oligosaccharides, 5-15 parts of sodium butyrate, 5-15 parts of citric acid, 5-15 parts of fumaric acid, 20-50 parts of maltodextrin, 5-15 parts of silicon dioxide and 60-95 parts of a montmorillonite powder carrier; and the plant essence oil mixture is prepared by mixing and blending the following raw materials: 4-10 parts of common origanum oil, 2-8 parts of clove oil and 2-8 parts of cinnamon oil. Being added into piglet feeds, the feed additive is capable of effectively improving piglet immunity, enhancing disease resistance and disease prevention abilities of the piglets, improving anti-stress abilities, reducing diarrhea occurrence rates of the piglets, and improving growth performances; the feed additive is free of any toxic side effects, and is safe and reliable to use; moreover, the feed additive can be used to replace veterinary antibiotics and other chemical synthetic drugs which are commonly used at present.

The invention provides a compound feed additive for improving anti-stress ability of a crucian. The compound feed additive comprises tea polyphenol, taurine, tryptophan, organic zinc, organic selenium, mannan oligosaccharide, vitamin C and a carrier. After adding the feed additive at a weight percentage of 0.2-0.3% to basic formula feed of the crucian under severe water quality conditions with 1-3mg/L dissolved oxygen and the concentration of ammonia nitrogen and nitrite being 0.1-1.5mg/L, a survival rate and a weight gain rate of the crucian are increased, a feed coefficient is reduced at the same time, and the culture efficiency is improved.

The invention belongs to the technical field of pet foods, and provides a chocolate-flavored yeast source pet nutrient solution and a preparation method thereof. The chocolate-flavored yeast source pet nutrient solution comprises the following components in parts by weight of 68.9-87.9 parts of water, 3-7 parts of enzymolysis chicken liver meal, 2-5 parts of enzymolysis chicken meat, 2-5 parts ofgoat milk powder, 1-3 parts of a thickening agent, 0.5-2.5 parts of taurine, 0.8-2.0 parts of calcium carbonate, 1 part of a compound premix, 0.5-2 parts of composite glue, 0.3-0.8 part of lecithin, 0.2-0.5 part of a yolk extract, 0.2-0.6 part of yeast beta-1, 3/1, 6-glucan, 0.2-0.6 part of mannan oligosaccharides, 0.2-0.6 part of rapeseed oil, 0.2-0.5 part of sodium tripolyphosphate and 0.1-0.3 part of monostearin. Through the technical scheme, the problems that in the prior art, pet nutrient supplementing products are poor in palatability and are poor in effects on improvement of the immunity of pets, and improving health of intestines of pets, are solved.

The invention discloses a compound micro-ecological preparation for aquaculture, and belongs to the field of micro-ecological preparations for aquaculture technologies. The compound micro-ecological preparation is prepared from compound microorganisms, a complex enzyme system and composite immunity enhancing factors, wherein the compound microorganisms comprise enterococcus faecalis, enterococcusfaecium, bacillus subtilis and saccharomyces cerevisiae, the complex enzyme system comprises protease, cellulase and lipase, and the composite immunity enhancing factors comprise mannan oligosaccharide, beta-glucan, chitosan oligosaccharide and peptidoglycan. The compound micro-ecological preparation has the advantages that the health problems of intestines and stomach in the existing breeding process can be effectively solved, the immunity and the disease-resistant and anti-stress capabilities can be enhanced, and green and healthy breeding can be protected.

The invention belongs to the feed processing field, and discloses a feed additive containing galactomannan-oligosaccharides prepared by an enzymatic method and a preparation process thereof. The feed additive takes galactomannan gum endosperm splits as raw materials, and the feed additive containing galactomannan-oligosaccharides is obtained after pretreatment, water dissolving and zymolysis. The preparation process has the advantages of simple process, no pollution, saving resources and the like. The product in the invention needs no refined purification and the raw materials are all from vegetable gum processed crude products, thus reducing cost greatly and being applicable to industrialized production.