71 results about "Manno-oligosaccharide" patented technology

The invention belongs to the field of agricultural chemistry and bio-fertilizer, and discloses a fertilizer synergist based on polyaspartic acid, alginate oligosaccharide and mannan oligosaccharide and a preparation method of the fertilizer synergist. According to the synergist, polyaspartic acid derived from plant source amino acid, alginate oligosaccharide extracted from natural brown algae and mannan oligosaccharide extracted from plants are used as main raw materials, and the polyaspartic acid, the alginate oligosaccharide and the mannan oligosaccharide cooperate to form an induction-complexation-adsorption three-effect-in-one mechanism, wherein the alginate oligosaccharide and the mannan oligosaccharide induce plant root activity and stress resistance gene expression; the polyaspartic acid is dominated to form a multi-point weak complex network, so that slow release and controlled release of nitrogen and potassium ions are realized; the three components cooperate on the surface of soil colloid to form an adsorption layer, and nutrient leaching loss is reduced. The preparation process adopts a green process of'enzymatic pretreatment-segmented complexing-low-temperature forming ', so that the oligosaccharide activity is maintained, and the use of an organic solvent is avoided. The obtained synergist can significantly improve the nitrogen utilization rate and the root activity and prolong the fertilizer efficiency period, and is suitable for green and efficient agricultural production of various crops.

The invention belongs to the technical field of medicines and health-care products, and particularly relates to compound prebiotics with an intestinal tract improving function as well as a preparation method and application of the compound prebiotics. The compound prebiotics with the function of improving the intestinal tract, disclosed by the invention, are prepared from the following components in parts by mass: 2 to 5 parts of polydextrose, 1 to 2 parts of erythritol, 8 to 10 parts of oat beta-glucan, 4 to 6 parts of stachyose, 12 to 15 parts of beta-galactomannan oligosaccharide, 20 to 30 parts of isomaltooligosacharide and 2 to 4 parts of a multifunctional additive. The multifunctional additive comprises fructo-oligosaccharide, red wine polyphenol and fatty acid. The compound prebiotics provided by the invention not only can promote the growth and reproduction of probiotics, improve the micro-ecological balance in intestinal tracts, improve the immunity of the intestinal tracts and improve the functions of the intestinal tracts, but also can prevent diarrhea.

The invention discloses a preparation method of mannan oligosaccharide, and relates to the technical field of organic agricultural fertilizers. According to the method, high-purity and high-efficiency mannan oligosaccharide is prepared by using an advanced process, and the method comprises the following specific steps: pretreatment of raw materials: mixing konjaku flour or guar gum with water to obtain a colloidal solution; adding beta-mannase and alpha-galactosidase into the colloidal solution, and carrying out enzymolysis under the assistance of a pulsed electric field; performing secondary separation on the enzymatic hydrolysate through an ultrafiltration membrane and a nanofiltration membrane, and collecting trapped fluid. A calcium ion solution is added into the trapped fluid for a complex reaction, then spray drying is conducted, and mannan oligosaccharide powder is obtained. According to the method, mannan oligosaccharide with concentrated purity and polymerization degree is prepared through ultrahigh pressure homogenization, pulsed electric field assisted enzymolysis and membrane separation technologies, the method has the advantages of high efficiency and environmental protection, and the preparation efficiency and the product quality are remarkably improved. The prepared mannan oligosaccharide can be applied to organic agricultural fertilizers, and can be compounded with humic acid and other substances to improve the biological activity and soil fertility of the fertilizers.

The invention belongs to the technical field of agriculture, and provides synergistic urea as well as a preparation method and application thereof. The synergistic urea provided by the invention comprises the following components in percentage by weight: 98.8 to 99.7 percent of urea and 0.3 to 1.2 percent of synergist (comprising polyaspartic acid, zinc iminodisuccinate and mannan oligosaccharide). When the synergistic urea is applied to soil, compared with urea, the synergist permeates into the soil preferentially, and polyaspartic acid can adsorb the urea and ammonium nitrogen and nitrate nitrogen converted from the urea, so that the nitrogen fixation effect is achieved, and the loss of nitrogen is reduced. Meanwhile, the polyaspartic acid, the zinc iminodisuccinate and the mannan oligosaccharide act together, so that the growth of crop roots can be promoted, the demand of the crop roots on nitrogen is improved, and the absorption of the crops on nitrogen in the soil is improved. Besides, the polyaspartic acid, the zinc iminodisuccinate and the mannan oligosaccharide act together, so that the activity of enzymes in crops can be improved, the crops can efficiently utilize low-concentration nitrogen, and the effective utilization rate of nitrogen is increased.

The invention discloses a method for preparing high-purity mannan oligosaccharide from konjac fine powder, which comprises the following steps: washing the konjac fine powder with alcohol, treating with alpha-amylase and glucoamylase, degrading konjac glucomannan by using a compound enzyme consisting of beta-glucanase and beta-mannase, and finally adjusting the pH value to an isoelectric point to prepare the high-purity konjac mannan oligosaccharide. According to the method, high-content impurities such as starch, protein and alkaloid in the konjak fine powder are removed, the problem of accumulation control of konjak processing byproducts is solved, the utilization rate and the economic value of konjak resources are improved, and the source of mannan oligosaccharide is enriched. The method realizes recycling of the konjac fine powder, has the advantages of being simple in process, low in equipment requirement and suitable for industrial production, and can be popularized and applied on a large scale.

The invention discloses a production process of anti-hair loss baked grain, which relates to the technical field of baked grain production, comprising 65% chicken, 20% salmon meat, 3% chicken liver, 3% chicken heart, 2% deep-sea fish oil, 2% Antarctic krill oil, brewer's yeast powder, 1% chicken fat, mixed fruit and vegetable powder, cellulose, yucca powder; additive composition: 0.3% manno-oligosaccharide, 0.3% xylo-oligosaccharide, egg yolk lecithin, calcium hydrogen phosphate, serine, sodium hyaluronate for pets, taurine, lysine, methionine, tyrosine, glutamine, chloride Choline, vitamin A, vitamin D3, vitamin E, niacinamide, D-calcium pantothenate, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, biotin, protein zinc, protein iron, protein copper, protein manganese, rosemary extract, tea polyphenols, S1, raw and auxiliary materials collection: S2, meat paste enzymatic hydrolysis, add the minced material into the enzymatic hydrolysis tank, the enzymatic hydrolysis time is one hour, the temperature is 50°-60°, after reaching the specified time, the temperature is raised to inactivate the enzyme, and the material is discharged at the lower temperature. The present invention has the characteristics of high process integration.

This invention discloses a pet food with coat-enhancing, shine-promoting, and coat-protecting effects, its preparation method, and applications. The food comprises the following components: chicken meal, fresh chicken meat, duck meal, sardine meal, chicken liver, sweet potato meal, corn meal, chicken heart, beef bone meal, duck meat pear, chicken oil, and egg yolk granules; coriander root powder, inulin, seaweed powder, beet pulp, flaxseed, fish oil, cranberry powder, carrot, krill, honeysuckle, turmeric, chrysanthemum, mannan oligosaccharide, lactulose, etc. The raw materials and auxiliary ingredients of this pet food are widely available, palatable, inexpensive, and readily available. The coriander root powder, other auxiliary ingredients, and nutritional supplements / additives work synergistically to improve the shine of the pet's coat, promote growth, and maintain coat health, while also improving the microstructure of the hair cuticle, fundamentally preventing pet coat health problems. The pet food preparation method disclosed in this invention is simple to operate, safe, and environmentally friendly, and is particularly suitable for feed products with high humidity and high heat sensitivity.

The invention provides a mannan oligosaccharide fertilizer synergist with a biological synergistic function and a high-utilization-rate fertilizer. The mannan oligosaccharide fertilizer synergist comprises phosphorylated modified mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide and mannan oligosaccharide, the phosphorylated modified mannan oligosaccharide is obtained by carrying out phosphorylation reaction on mannan oligosaccharide through a phosphorylation reagent. According to the mannan oligosaccharide fertilizer synergist with the biological synergistic function and the high-utilization-rate fertilizer, mannan oligosaccharide is subjected to phosphorylation reaction to obtain phosphorylated modified mannan oligosaccharide which serves as the fertilizer synergist, compared with mannan oligosaccharide or other saccharides, the affinity of the phosphorylated modified mannan oligosaccharide with biomacromolecules such as films, enzymes and proteins is greatly improved, and the utilization rate of the fertilizer synergist is increased; while biological synergism is realized, absorption of nitrogen, phosphorus and potassium can be promoted, and the utilization rate of the fertilizer is increased.

The application provides a yeast mannose oligosaccharide and a preparation method and application thereof. The preparation method comprises the following steps: S1, mixing yeast cell walls with water to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 4-9, performing first enzymolysis on the mixed solution by using a protease to form a first enzymolysis product; secondly, performing second enzymolysis on the first enzymolysis product by using a polysaccharide enzyme to obtain a second enzymolysis product; the amount of the protease is greater than or equal to 0.2% of the weight of the yeast cell walls, and the amount of the polysaccharide enzyme is greater than or equal to 0.1% of the weight of the yeast cell walls; S3, centrifuging the second enzymolysis product to obtain a supernatant and a centrifugal heavy phase; filtering the supernatant by using an ultrafiltration membrane, and the retentate is the yeast mannose oligosaccharide, the permeate is a yeast extract, and the centrifugal heavy phase is a yeast glucan. The yeast mannose oligosaccharide obtained by the application has high purity and high yield, the mannose oligosaccharide is clean, can be fermented by beneficial bacteria, and can be applied as a prebiotic in food.

To provide a functional composition reduced in sulfur dioxide in a composition containing mannose and / or mannooligosaccharide.SOLUTION: The sulfur dioxide concentration in the functional composition can be reduced by decomposing devil's tongue powder derived from devil's tongue as a raw material and then heat-treating the resultant composition containing mannose and / or mannooligosaccharide at ≥ 105 °C. The functional composition with reduced sulfur dioxide (sulfurous acid) can be distributed as food and drink without legal problems.SELECTED DRAWING: None

FIELD: veterinary medicine and dietetics.SUBSTANCE: development of complete feed compositions that can provide a therapeutic effect in cases of exocrine pancreatic insufficiency. The composition of a complete dietary feed for cats with exocrine pancreatic insufficiency has been developed in the following ratio, wt.%: grain part: mixture of rice, wheat, hulled barley and corn – 37.0; protein part of plant origin: isolated soy protein, beet pulp, wheat bran, flax seeds – 22.0; protein part of animal origin: feather meal, chicken liver, egg powder – 25.0; mineral supplement: feed chalk, monocalcium phosphate, table salt, plantain seeds Plantago psyllium, mannanoligasaccharides with beta-glucans and lutein – 2.0; vitamin premix: A, D3, E, K, B1, B2, B3, B5, B6, B12, Bc, H, taurine, inulin, beech lignocellulose, complex of natural fulvic and humic acids – 2.5; flavor enhancer in the form of enzymatic hydrolysate of pork and chicken by-products – 3–3.5; brewer's yeast – 2.5–3.0; a mixture of fish and chicken fat – 5.0–6.0.EFFECT: obtaining food for cats with exocrine pancreatic insufficiency, which is easily reproducible, clinically effective, and convenient in dosing and use.1 cl, 3 tbl, 1 ex

The application provides a method for improving the LPS stress resistance of broilers, which is realized by feeding a mannose oligosaccharide compound preparation prepared from enzymolysis coffee grounds. The preparation method of the mannose oligosaccharide compound preparation comprises the following steps: crushing coffee grounds, and adding a compound reagent for pretreatment; adding a compound enzyme preparation I to the mixed system after reaction, and carrying out enzymolysis for 6-8 hours under the condition of 50-60 DEG C; then adding a compound enzyme preparation II, and carrying out sufficient enzymolysis under the condition of 55-65 DEG C; after the enzymolysis is completed, a fermentation compound microbial agent is used for continuing fermentation for 6-12 hours under the condition of 30-35 DEG C, and drying is carried out; the compound enzyme preparation I comprises papain, alpha-amylase and saccharifying enzyme; the compound enzyme preparation II comprises cellulase, mannanase and glucanase; and the fermentation compound microbial agent comprises maxwell kluyver yeast and lactobacillus plantarum. The method can not only avoid drug residues caused by feeding drugs, but also realize the reuse of coffee grounds.

The invention discloses a roxburgh rose lactic acid bacteria solid beverage and application thereof in preparation of foods, health-care products or medicines for reducing hyperlipidemia, preventing colitis and resisting oxidation, and belongs to the field of probiotic health-care foods. The rosa roxburghii tratt lactic acid bacteria solid beverage is prepared from plant lactobacillus R-15, rosa roxburghii tratt fine powder, mannan oligosaccharide, resistant dextrin, coix lacryma-jobi extract, hawthorn extract, silicon dioxide and the like. The rosa roxburghii tratt lactobacillus solid beverage can significantly reduce the levels of triglyceride, total cholesterol and low-density lipoprotein cholesterol in the liver and serum of a mouse, inhibit fatty degeneration of the liver, and inhibit colitis induced by high fat diet. The invention has important application value in prevention and treatment of hyperlipidemia and colitis.

The invention discloses a mannase mutant as well as a coding gene and application thereof. The invention provides a mannase mutant which is any one of the following proteins: A1) the amino acid sequence of the protein is obtained by mutating the 38th Gln of the amino acid sequence of wild type mannase as shown in a sequence 3 into Lys, mutating the 224th Asp into Ala and mutating the 272th Ala into Thr, and the amino acid sequence of the protein is obtained by mutating the 38th Gln of the amino acid sequence of wild type mannase as shown in the sequence 3 into Lys, mutating the 224th Asp of the amino acid sequence of wild type mannase as shown in the sequence 3 into Thr; a2) a fusion protein obtained by connecting the N end or / and C end of the protein shown in A1) with a protein tag. The mannase mutant provided by the invention is derived from aspergillus niger, has good enzymatic properties and hydrolysis characteristics, can efficiently hydrolyze guar bean slices under a natural pH value condition to generate mannan oligosaccharide, and has great application potential in the field of food.

The invention discloses a salt-reducing freshness-enhancing high-temperature-resistant functional seasoning composition beneficial to maintaining intestinal microecological balance, and a preparation method and application thereof, and belongs to the technical field of food seasoning. The core of the composition is a synergistic dietary fiber combination composed of yeast beta-glucan, yeast mannan oligosaccharide and resistant dextrin according to the weight ratio of (3-5): (4-6): (2-4), edible salt and yeast extract are matched, the sodium content of the product is smaller than or equal to 14000mg / 100g, when clear soup is prepared according to the addition amount of 0.5% (w / v), the delicate flavor strength equivalent to that of traditional chicken essence can be kept, and the delicate flavor attenuation rate is smaller than or equal to 6.7% after heating is conducted for 60 min at the temperature of 100 DEG C. The product simultaneously realizes 0 sugar, 0 fat, low purine and sodium-potassium ratio balance, can synergistically promote proliferation of beneficial bacteria in intestinal tracts, solves the problem that salt reduction, fresh keeping, high temperature resistance and flavor stability of the existing seasoning are difficult to consider at the same time, and is suitable for industrial production.

The invention discloses a Saccharomyces mirabilis fermentation broth and a preparation method and application thereof, and the preparation method comprises the following steps: crushing or homogenizing coconut solid endosperm to prepare a fermentation medium; inoculating saccharomyces cerevisiae to the fermentation culture medium for fermentation; and after the fermentation is finished, carrying out separation treatment to obtain the saccharomyces cerevisiae fermentation liquor. Compared with the prior art, the saccharomyces mirabilis is selected as a fermentation strain, the coconut solid endosperm is taken as a main fermentation substrate, the metabolic characteristics of the strain are fully exerted under a mild fermentation condition, and the mannan in the coconut solid endosperm is efficiently converted into mannan oligosaccharide; the obtained saccharomyces cerevisiae fermentation liquor has excellent moisturizing and repairing effects and the like, and has a good application prospect in cosmetics.

The invention discloses a pediococcus pentosaceus microcapsule and a preparation method thereof. The composite embedding agent is prepared by an endogenous emulsification method. According to the microcapsule prepared by the invention, the storage stability, the heat resistance and the antibacterial activity of the pentose tablet ball STS-6 are remarkably improved. The mannan oligosaccharide and the sodium alginate are combined for use, the pullulan and the poly gamma-glutamic acid are combined for use, and the schizophyllum commune polysaccharide and the rice bran protein are combined for use for synergistic interaction.

The invention relates to a fertilizer synergist and a preparation method thereof, in particular to a fertilizer synergist which is based on natural components such as modified aspartic acid, alginate oligosaccharide, mannan oligosaccharide and magnetic polysaccharide and takes a fertilizer as a carrier. According to the fertilizer synergist, through the innovative modification of the modified aspartic acid and the synergistic effect of the natural polysaccharide, the utilization efficiency and the slow release property of a fertilizer and the nutrient absorption capacity of crops are remarkably improved. Compared with the traditional fertilizer synergist, the synergist disclosed by the invention can prolong the release time of nutrients, reduce the loss of the fertilizer and improve the yield of crops, has better environmental friendliness, and reduces possible pollution to soil and water in the fertilizer application process. The fertilizer synergist disclosed by the invention has remarkable advantages in the aspects of improving the fertilizer utilization rate, crop growth and soil environmental protection. The fertilizer synergist provides an efficient, environment-friendly and sustainable technical solution for agricultural production.

The present application relates to a kind of complex enzyme preparation for inhibiting zearalenone generation and its preparation method and application, belong to microorganism and food safety technical field.The complex enzyme preparation prepared in the present application contains medium-temperature alpha-amylase, glucose oxidase, mannose oligosaccharide and soluble starch, medium-temperature alpha-amylase can effectively inhibit the generation of zearalenone in peanut meal, glucose oxidase can consume oxygen and produce acid, inhibit the growth of mould, mannose oligosaccharide can efficiently adsorb the generated zearalenone, and soluble starch is a stable carrier, which can be enzymatically hydrolyzed by medium-temperature alpha-amylase to generate glucose, providing a slow-release substrate for glucose oxidase.The complex enzyme preparation product can inhibit the generation of mycotoxin in crop feed raw materials from the root, has little effect on product quality, is more friendly to the environment, and the raw materials of complex enzyme preparation are easy to obtain, low in cost and widely used.

The invention provides a compound urea cream and a preparation method thereof, the core raw materials comprise 8-12 parts of urea, 0.5-1.2 parts of mannan oligosaccharide / fructo-oligosaccharide / inulin prebiotics in a specific proportion, and 0.3-0.8 part of a transdermal repair agent, the core raw materials are supplemented with bisabolol, allantoin, vitamin E acetate, dipotassium glycyrrhizinate and the like, and a hexadecanol / octadecanol mixed emulsifier and liquid paraffin are used as an oil phase. The preparation process comprises the following steps: encapsulating urea by using alhagi sparsifolia horny layer wax to form microcapsules; an oil phase and a water phase are uniformly dispersed by adopting pulse type homogeneous alternation, and a stable system with the particle size of 1-5 microns is formed; the cream inhibits urea crystal precipitation, controls the slow release rate, effectively enhances the hydration of cuticles, improves the moisturizing and water locking capabilities, has the functions of efficient moisturizing, anti-inflammation and barrier repair, and provides a new scheme for skin disease treatment.

The invention relates to the technical field of functional feed additive development, in particular to a feed formula and a process for improving yellow tripe of fattening sheep, a novel selenium-porphyrin complex is used for resisting oxidation, an extract rich in beta-glucan and mannan oligosaccharide is used for regulating immunity and intestinal flora, a natural plant polyphenol compound is used for chelating heavy metals, and the feed has the effects of improving immunity and improving the yellow tripe of fattening sheep. The nano-scale montmorillonite and probiotic compound preparation can regulate intestinal tracts, the special amino acid chelated iron can supplement iron, and basic raw materials and other additives are added. The feed disclosed by the invention can efficiently improve the yellow tripe of the fattening sheep, and improves the growth performance and mutton quality of the fattening sheep by regulating nutrition metabolism, enhancing immunity, improving intestinal health, improving oxidation resistance and reducing the incidence rate of the yellow tripe, so that a synergistic effect is provided for farmers.

The invention discloses a fattening sheep compound premix for regulating intestinal flora and a preparation method thereof, and each kilogram of the compound premix consists of the following components in parts by weight: 80-120g of a micro-ecological regulation component, 20-30g of an amino acid composition, 5-10g of compound vitamins, 30-50g of compound minerals, 15-25g of a Chinese herbal medicine extract and 745-850g of a carrier, according to the fattening sheep compound premix capable of regulating the intestinal flora and the preparation method of the fattening sheep compound premix, regulation and control are performed through probiotics, prebiotics and Chinese herbal medicine extracts, lactobacillus plantarum and bacillus subtilis can directionally proliferate intestinal beneficial bacteria and inhibit growth of harmful bacteria such as escherichia coli and staphylococcus aureus, and saccharomyces cerevisiae culture assists in regulating intestinal microecological balance, so that the intestinal flora can be regulated and controlled, and the intestinal flora can be regulated and controlled. The galactomannan oligosaccharide is used as prebiotics and can promote colonization and proliferation of probiotics and prolong the action time of the probiotics in intestinal tracts, and the Chinese herbal medicine extract and the micro-ecological components cooperate to assist in regulating the intestinal function and enhance the immunity of the organism.

The invention discloses a composite micro-ecological preparation and application thereof in feed, and belongs to the technical field of microorganisms, the composite micro-ecological preparation comprises lysinibacillus and mannan oligosaccharide, the lysinibacillus is preserved in the China Center for Type Microbiological Culture Collection, the preservation address is Wuhan University, Hubei Province, and the preservation number is CCTCC No.M20241816. The invention further discloses a preparation method of the composite micro-ecological preparation. The compound microecological preparation disclosed by the invention can be used for more remarkably reducing the content of aflatoxin B1 and increasing the content of water-soluble polysaccharide, and is beneficial to increasing the market value of a feed containing peanut meal, corn and / or soybean meal.

The invention discloses a pet nutritional composition capable of reducing peculiar smell of excrements and a preparation method of the pet nutritional composition. The pet nutritional composition is prepared by the following steps: performing pre-emulsification and high-shear emulsification on functional components such as ethyl lactate, pig blood protein peptide, a pericarpium citri reticulatae extract, a yucca extract and phytic acid; the microcapsule is encapsulated in a sodium alginate-calcium chloride gel network integrated with nano zeolite powder and agaro-oligosaccharide through ionic gelation to form a functional microcapsule base material; meanwhile, the lactobacillus is embedded in the sodium alginate-chitosan composite gel microcapsule by adopting an emulsification-internal gelation technology. And finally, mixing the prebiotics with mannan oligosaccharide and fructo-oligosaccharide compounded prebiotics. Through split type embedding and differentiated wall material design, the problems that probiotics are prone to inactivation and components interfere with one another are solved, intestinal tract targeted rapid release of functional components and sequential precise delivery of the probiotics are achieved, a triple deodorization mechanism of source inhibition, efficient adsorption and micro-ecological regulation is synergistically exerted, and the deodorization effect is good. The deodorization efficiency and the action durability are obviously improved.

The invention provides high-temperature mannase and application thereof. The amino acid sequence of the high-temperature mannase is SEQ ID NO: 1. The mannase has the strongest hydrolysis effect on konjac gum, can hydrolyze guar gum and has a wide acting temperature and pH range, the enzyme activity is kept at 95% or above within the range of 45-75 DEG C, the enzyme activity is kept at 65% or above within the range of pH 5.5-8.5, and various common metal ions can promote the enzyme activity or have little influence on the enzyme activity. The enzyme is beta-mannase with endo-activity, and has a good application prospect in the aspects of feed processing, mannan oligosaccharide preparation, biological polishing, agricultural waste utilization, plant active substance extraction and the like under medium and high temperature conditions.

The invention relates to the technical field of pharmaceutical compositions, and particularly discloses a pharmaceutical composition for animal parasite expelling and a preparation method and application thereof. The pharmaceutical composition comprises the following raw material components in parts by mass: 15-30 parts of a traditional Chinese medicine extracting solution, 5-10 parts of doxycycline hydrochloride, 1-5 parts of vitamin C and 1-5 parts of mannan oligosaccharide, wherein the traditional Chinese medicine extracting solution comprises the following raw material components: a dark plum extracting solution, a pepper extracting solution, a wild buckwheat extracting solution, a gemma agrimoninae extracting solution, a rhizoma smilacis glabrae extracting solution and a cortex phellodendri extracting solution. The pharmaceutical composition for animal parasite expelling provided by the invention not only has an excellent parasite expelling effect, but also has the advantages of safe raw material components, no toxic or side effect on animal bodies, and difficult generation of drug resistance. According to the technical scheme, the problems that in the prior art, drugs for preventing and treating animal parasitic diseases are large in toxic and side effects, prone to generating drug resistance, poor in parasite expelling effect and narrow in prevention and treatment range are effectively solved.

The invention belongs to the technical field of feed product preparation, and particularly relates to a composite functional feed additive and application thereof. The feed contains a compound functional feed additive, and the compound functional feed additive comprises the following components: 1-2 parts of Vc-phosphate, 1-2 parts of taurine, 1-2 parts of betaine, 0.4-0.5 part of bile acid, 0.4-0.5 part of mannan oligosaccharide, 0.1-0.2 part of beta-glucan, 0.4-0.5 part of saccharomyces cerevisiae cell wall, 1-2 parts of sodium butyrate and 8-10 parts of a compound traditional Chinese medicine preparation. The compound traditional Chinese medicine preparation contains scutellaria baicalensis, rheum officinale, golden cypress, astragalus membranaceus, folium isatidis, houttuynia cordata, isatis root, radix paeoniae alba, radix bupleuri and gynostemma pentaphylla. Through combined use of the functional feed additive, the feed utilization efficiency of the largemouth bass and the ictalurus punctatus juvenile fish can be effectively improved, and the physique is enhanced, so that the growth performance is improved, and the culture benefit is improved.

The invention provides a poecilobdella manillensis breeding feed and a preparation method thereof, and relates to the technical field of aquaculture. The poecilobdella manillensis breeding feed comprises the following components: animal blood freeze-dried powder, soybean meal, phenylalanine, isoleucine, aspartic acid, mannan oligosaccharide, a traditional Chinese medicine extract, syringin, magnesium lignosulfonate, vitamins and fishy flavor essence. According to the feed for feeding poecilobdella manillensis, by optimizing the components, the proportion and the preparation method, the survival rate, the body weight growth rate and the antithrombin activity growth rate of the poecilobdella manillensis are remarkably increased, and the prevalence rate of the poecilobdella manillensis is reduced.

The invention discloses a synthetic method and application of antrodia camphorata galactomannan oligosaccharide and polysaccharide, and belongs to the field of carbohydrate chemistry and medicinal chemistry. According to the method, five tetrasaccharide building blocks are used as synthons, the construction efficiency of antrodia camphorata galactose 1, 2-cis-glucosidic bonds is improved through remote participation of galactose 4-position benzoyl, and octasaccharide, hexadecanose and tetracosanose of antrodia camphorata are stereoselectively constructed through [4 + 4], [8 + 8] and [16 + 8] glycosylation strategies by utilizing the method; the obtained oligosaccharides and polysaccharides are subjected to deprotection to obtain six antrodia camphorata galactomannan oligosaccharides and polysaccharides with definite structures, single components and anti-inflammatory activity. A cellular immune experiment shows that the oligosaccharide and the polysaccharide can inhibit LPS (lipopolysaccharide)-induced mouse macrophage RAW 264.7 inflammatory response, and have potential application in anti-inflammatory drugs; meanwhile, the antigens are good antigens of antrodia camphorata sugar vaccines and can be developed into sugar vaccines or sugar drugs.

The invention discloses a cactus external preparation and a preparation method thereof, and relates to the technical field of skin external preparations and preparation thereof.The cactus external preparation comprises, by mass, 4.5% of a cactus oligosaccharide compound, the molar ratio of mannan oligosaccharide to arabinogalactan in the cactus oligosaccharide compound is 3: 2, and the cactus oligosaccharide compound contains beta-1, 4 glucosidic bond modification groups; 6% of sericin temperature-sensitive microspheres, the particle size of the microspheres being 200-300 nm, and the phase transition temperature being 32-35 DEG C; according to the invention, staphylococcus epidermidis is activated to secrete antibacterial substances in a targeted manner through the cactus oligosaccharide compound, a physical barrier is formed in combination with the sericin temperature-sensitive microspheres, lactobacillus extracellular vesicles inhibit metabolism of propionibacterium acnes, glycerol caprylate selectively interferes with the fluidity of a pathogenic bacterium membrane, and konjac glucomannan regulates immunity; and a flora regulation and control-barrier protection-immune regulation system is constructed under the assistance of a pulsed electric field pretreatment process and the like, so that the problems of flora imbalance and drug resistance caused by traditional sterilization are solved, and skin micro-ecological balance and inflammation blocking are realized.