45 results about "Manno-oligosaccharide" patented technology

The invention belongs to the field of agricultural chemistry and bio-fertilizer, and discloses a fertilizer synergist based on polyaspartic acid, alginate oligosaccharide and mannan oligosaccharide and a preparation method of the fertilizer synergist. According to the synergist, polyaspartic acid derived from plant source amino acid, alginate oligosaccharide extracted from natural brown algae and mannan oligosaccharide extracted from plants are used as main raw materials, and the polyaspartic acid, the alginate oligosaccharide and the mannan oligosaccharide cooperate to form an induction-complexation-adsorption three-effect-in-one mechanism, wherein the alginate oligosaccharide and the mannan oligosaccharide induce plant root activity and stress resistance gene expression; the polyaspartic acid is dominated to form a multi-point weak complex network, so that slow release and controlled release of nitrogen and potassium ions are realized; the three components cooperate on the surface of soil colloid to form an adsorption layer, and nutrient leaching loss is reduced. The preparation process adopts a green process of'enzymatic pretreatment-segmented complexing-low-temperature forming ', so that the oligosaccharide activity is maintained, and the use of an organic solvent is avoided. The obtained synergist can significantly improve the nitrogen utilization rate and the root activity and prolong the fertilizer efficiency period, and is suitable for green and efficient agricultural production of various crops.

This invention discloses a pet food with coat-enhancing, shine-promoting, and coat-protecting effects, its preparation method, and applications. The food comprises the following components: chicken meal, fresh chicken meat, duck meal, sardine meal, chicken liver, sweet potato meal, corn meal, chicken heart, beef bone meal, duck meat pear, chicken oil, and egg yolk granules; coriander root powder, inulin, seaweed powder, beet pulp, flaxseed, fish oil, cranberry powder, carrot, krill, honeysuckle, turmeric, chrysanthemum, mannan oligosaccharide, lactulose, etc. The raw materials and auxiliary ingredients of this pet food are widely available, palatable, inexpensive, and readily available. The coriander root powder, other auxiliary ingredients, and nutritional supplements / additives work synergistically to improve the shine of the pet's coat, promote growth, and maintain coat health, while also improving the microstructure of the hair cuticle, fundamentally preventing pet coat health problems. The pet food preparation method disclosed in this invention is simple to operate, safe, and environmentally friendly, and is particularly suitable for feed products with high humidity and high heat sensitivity.

The invention provides a mannan oligosaccharide fertilizer synergist with a biological synergistic function and a high-utilization-rate fertilizer. The mannan oligosaccharide fertilizer synergist comprises phosphorylated modified mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide, mannan oligosaccharide and mannan oligosaccharide, the phosphorylated modified mannan oligosaccharide is obtained by carrying out phosphorylation reaction on mannan oligosaccharide through a phosphorylation reagent. According to the mannan oligosaccharide fertilizer synergist with the biological synergistic function and the high-utilization-rate fertilizer, mannan oligosaccharide is subjected to phosphorylation reaction to obtain phosphorylated modified mannan oligosaccharide which serves as the fertilizer synergist, compared with mannan oligosaccharide or other saccharides, the affinity of the phosphorylated modified mannan oligosaccharide with biomacromolecules such as films, enzymes and proteins is greatly improved, and the utilization rate of the fertilizer synergist is increased; while biological synergism is realized, absorption of nitrogen, phosphorus and potassium can be promoted, and the utilization rate of the fertilizer is increased.

The application provides a yeast mannose oligosaccharide and a preparation method and application thereof. The preparation method comprises the following steps: S1, mixing yeast cell walls with water to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 4-9, performing first enzymolysis on the mixed solution by using a protease to form a first enzymolysis product; secondly, performing second enzymolysis on the first enzymolysis product by using a polysaccharide enzyme to obtain a second enzymolysis product; the amount of the protease is greater than or equal to 0.2% of the weight of the yeast cell walls, and the amount of the polysaccharide enzyme is greater than or equal to 0.1% of the weight of the yeast cell walls; S3, centrifuging the second enzymolysis product to obtain a supernatant and a centrifugal heavy phase; filtering the supernatant by using an ultrafiltration membrane, and the retentate is the yeast mannose oligosaccharide, the permeate is a yeast extract, and the centrifugal heavy phase is a yeast glucan. The yeast mannose oligosaccharide obtained by the application has high purity and high yield, the mannose oligosaccharide is clean, can be fermented by beneficial bacteria, and can be applied as a prebiotic in food.

To provide a functional composition reduced in sulfur dioxide in a composition containing mannose and / or mannooligosaccharide.SOLUTION: The sulfur dioxide concentration in the functional composition can be reduced by decomposing devil's tongue powder derived from devil's tongue as a raw material and then heat-treating the resultant composition containing mannose and / or mannooligosaccharide at ≥ 105 °C. The functional composition with reduced sulfur dioxide (sulfurous acid) can be distributed as food and drink without legal problems.SELECTED DRAWING: None

FIELD: veterinary medicine and dietetics.SUBSTANCE: development of complete feed compositions that can provide a therapeutic effect in cases of exocrine pancreatic insufficiency. The composition of a complete dietary feed for cats with exocrine pancreatic insufficiency has been developed in the following ratio, wt.%: grain part: mixture of rice, wheat, hulled barley and corn – 37.0; protein part of plant origin: isolated soy protein, beet pulp, wheat bran, flax seeds – 22.0; protein part of animal origin: feather meal, chicken liver, egg powder – 25.0; mineral supplement: feed chalk, monocalcium phosphate, table salt, plantain seeds Plantago psyllium, mannanoligasaccharides with beta-glucans and lutein – 2.0; vitamin premix: A, D3, E, K, B1, B2, B3, B5, B6, B12, Bc, H, taurine, inulin, beech lignocellulose, complex of natural fulvic and humic acids – 2.5; flavor enhancer in the form of enzymatic hydrolysate of pork and chicken by-products – 3–3.5; brewer's yeast – 2.5–3.0; a mixture of fish and chicken fat – 5.0–6.0.EFFECT: obtaining food for cats with exocrine pancreatic insufficiency, which is easily reproducible, clinically effective, and convenient in dosing and use.1 cl, 3 tbl, 1 ex

The application provides a method for improving the LPS stress resistance of broilers, which is realized by feeding a mannose oligosaccharide compound preparation prepared from enzymolysis coffee grounds. The preparation method of the mannose oligosaccharide compound preparation comprises the following steps: crushing coffee grounds, and adding a compound reagent for pretreatment; adding a compound enzyme preparation I to the mixed system after reaction, and carrying out enzymolysis for 6-8 hours under the condition of 50-60 DEG C; then adding a compound enzyme preparation II, and carrying out sufficient enzymolysis under the condition of 55-65 DEG C; after the enzymolysis is completed, a fermentation compound microbial agent is used for continuing fermentation for 6-12 hours under the condition of 30-35 DEG C, and drying is carried out; the compound enzyme preparation I comprises papain, alpha-amylase and saccharifying enzyme; the compound enzyme preparation II comprises cellulase, mannanase and glucanase; and the fermentation compound microbial agent comprises maxwell kluyver yeast and lactobacillus plantarum. The method can not only avoid drug residues caused by feeding drugs, but also realize the reuse of coffee grounds.

The invention discloses a roxburgh rose lactic acid bacteria solid beverage and application thereof in preparation of foods, health-care products or medicines for reducing hyperlipidemia, preventing colitis and resisting oxidation, and belongs to the field of probiotic health-care foods. The rosa roxburghii tratt lactic acid bacteria solid beverage is prepared from plant lactobacillus R-15, rosa roxburghii tratt fine powder, mannan oligosaccharide, resistant dextrin, coix lacryma-jobi extract, hawthorn extract, silicon dioxide and the like. The rosa roxburghii tratt lactobacillus solid beverage can significantly reduce the levels of triglyceride, total cholesterol and low-density lipoprotein cholesterol in the liver and serum of a mouse, inhibit fatty degeneration of the liver, and inhibit colitis induced by high fat diet. The invention has important application value in prevention and treatment of hyperlipidemia and colitis.

The invention discloses a mannase mutant as well as a coding gene and application thereof. The invention provides a mannase mutant which is any one of the following proteins: A1) the amino acid sequence of the protein is obtained by mutating the 38th Gln of the amino acid sequence of wild type mannase as shown in a sequence 3 into Lys, mutating the 224th Asp into Ala and mutating the 272th Ala into Thr, and the amino acid sequence of the protein is obtained by mutating the 38th Gln of the amino acid sequence of wild type mannase as shown in the sequence 3 into Lys, mutating the 224th Asp of the amino acid sequence of wild type mannase as shown in the sequence 3 into Thr; a2) a fusion protein obtained by connecting the N end or / and C end of the protein shown in A1) with a protein tag. The mannase mutant provided by the invention is derived from aspergillus niger, has good enzymatic properties and hydrolysis characteristics, can efficiently hydrolyze guar bean slices under a natural pH value condition to generate mannan oligosaccharide, and has great application potential in the field of food.

The invention discloses a salt-reducing freshness-enhancing high-temperature-resistant functional seasoning composition beneficial to maintaining intestinal microecological balance, and a preparation method and application thereof, and belongs to the technical field of food seasoning. The core of the composition is a synergistic dietary fiber combination composed of yeast beta-glucan, yeast mannan oligosaccharide and resistant dextrin according to the weight ratio of (3-5): (4-6): (2-4), edible salt and yeast extract are matched, the sodium content of the product is smaller than or equal to 14000mg / 100g, when clear soup is prepared according to the addition amount of 0.5% (w / v), the delicate flavor strength equivalent to that of traditional chicken essence can be kept, and the delicate flavor attenuation rate is smaller than or equal to 6.7% after heating is conducted for 60 min at the temperature of 100 DEG C. The product simultaneously realizes 0 sugar, 0 fat, low purine and sodium-potassium ratio balance, can synergistically promote proliferation of beneficial bacteria in intestinal tracts, solves the problem that salt reduction, fresh keeping, high temperature resistance and flavor stability of the existing seasoning are difficult to consider at the same time, and is suitable for industrial production.

The invention discloses a Saccharomyces mirabilis fermentation broth and a preparation method and application thereof, and the preparation method comprises the following steps: crushing or homogenizing coconut solid endosperm to prepare a fermentation medium; inoculating saccharomyces cerevisiae to the fermentation culture medium for fermentation; and after the fermentation is finished, carrying out separation treatment to obtain the saccharomyces cerevisiae fermentation liquor. Compared with the prior art, the saccharomyces mirabilis is selected as a fermentation strain, the coconut solid endosperm is taken as a main fermentation substrate, the metabolic characteristics of the strain are fully exerted under a mild fermentation condition, and the mannan in the coconut solid endosperm is efficiently converted into mannan oligosaccharide; the obtained saccharomyces cerevisiae fermentation liquor has excellent moisturizing and repairing effects and the like, and has a good application prospect in cosmetics.

The invention relates to a fertilizer synergist and a preparation method thereof, in particular to a fertilizer synergist which is based on natural components such as modified aspartic acid, alginate oligosaccharide, mannan oligosaccharide and magnetic polysaccharide and takes a fertilizer as a carrier. According to the fertilizer synergist, through the innovative modification of the modified aspartic acid and the synergistic effect of the natural polysaccharide, the utilization efficiency and the slow release property of a fertilizer and the nutrient absorption capacity of crops are remarkably improved. Compared with the traditional fertilizer synergist, the synergist disclosed by the invention can prolong the release time of nutrients, reduce the loss of the fertilizer and improve the yield of crops, has better environmental friendliness, and reduces possible pollution to soil and water in the fertilizer application process. The fertilizer synergist disclosed by the invention has remarkable advantages in the aspects of improving the fertilizer utilization rate, crop growth and soil environmental protection. The fertilizer synergist provides an efficient, environment-friendly and sustainable technical solution for agricultural production.

The present application relates to a kind of complex enzyme preparation for inhibiting zearalenone generation and its preparation method and application, belong to microorganism and food safety technical field.The complex enzyme preparation prepared in the present application contains medium-temperature alpha-amylase, glucose oxidase, mannose oligosaccharide and soluble starch, medium-temperature alpha-amylase can effectively inhibit the generation of zearalenone in peanut meal, glucose oxidase can consume oxygen and produce acid, inhibit the growth of mould, mannose oligosaccharide can efficiently adsorb the generated zearalenone, and soluble starch is a stable carrier, which can be enzymatically hydrolyzed by medium-temperature alpha-amylase to generate glucose, providing a slow-release substrate for glucose oxidase.The complex enzyme preparation product can inhibit the generation of mycotoxin in crop feed raw materials from the root, has little effect on product quality, is more friendly to the environment, and the raw materials of complex enzyme preparation are easy to obtain, low in cost and widely used.

The invention discloses a fattening sheep compound premix for regulating intestinal flora and a preparation method thereof, and each kilogram of the compound premix consists of the following components in parts by weight: 80-120g of a micro-ecological regulation component, 20-30g of an amino acid composition, 5-10g of compound vitamins, 30-50g of compound minerals, 15-25g of a Chinese herbal medicine extract and 745-850g of a carrier, according to the fattening sheep compound premix capable of regulating the intestinal flora and the preparation method of the fattening sheep compound premix, regulation and control are performed through probiotics, prebiotics and Chinese herbal medicine extracts, lactobacillus plantarum and bacillus subtilis can directionally proliferate intestinal beneficial bacteria and inhibit growth of harmful bacteria such as escherichia coli and staphylococcus aureus, and saccharomyces cerevisiae culture assists in regulating intestinal microecological balance, so that the intestinal flora can be regulated and controlled, and the intestinal flora can be regulated and controlled. The galactomannan oligosaccharide is used as prebiotics and can promote colonization and proliferation of probiotics and prolong the action time of the probiotics in intestinal tracts, and the Chinese herbal medicine extract and the micro-ecological components cooperate to assist in regulating the intestinal function and enhance the immunity of the organism.

The invention discloses a composite micro-ecological preparation and application thereof in feed, and belongs to the technical field of microorganisms, the composite micro-ecological preparation comprises lysinibacillus and mannan oligosaccharide, the lysinibacillus is preserved in the China Center for Type Microbiological Culture Collection, the preservation address is Wuhan University, Hubei Province, and the preservation number is CCTCC No.M20241816. The invention further discloses a preparation method of the composite micro-ecological preparation. The compound microecological preparation disclosed by the invention can be used for more remarkably reducing the content of aflatoxin B1 and increasing the content of water-soluble polysaccharide, and is beneficial to increasing the market value of a feed containing peanut meal, corn and / or soybean meal.

The invention discloses a pet nutritional composition capable of reducing peculiar smell of excrements and a preparation method of the pet nutritional composition. The pet nutritional composition is prepared by the following steps: performing pre-emulsification and high-shear emulsification on functional components such as ethyl lactate, pig blood protein peptide, a pericarpium citri reticulatae extract, a yucca extract and phytic acid; the microcapsule is encapsulated in a sodium alginate-calcium chloride gel network integrated with nano zeolite powder and agaro-oligosaccharide through ionic gelation to form a functional microcapsule base material; meanwhile, the lactobacillus is embedded in the sodium alginate-chitosan composite gel microcapsule by adopting an emulsification-internal gelation technology. And finally, mixing the prebiotics with mannan oligosaccharide and fructo-oligosaccharide compounded prebiotics. Through split type embedding and differentiated wall material design, the problems that probiotics are prone to inactivation and components interfere with one another are solved, intestinal tract targeted rapid release of functional components and sequential precise delivery of the probiotics are achieved, a triple deodorization mechanism of source inhibition, efficient adsorption and micro-ecological regulation is synergistically exerted, and the deodorization effect is good. The deodorization efficiency and the action durability are obviously improved.

The invention provides high-temperature mannase and application thereof. The amino acid sequence of the high-temperature mannase is SEQ ID NO: 1. The mannase has the strongest hydrolysis effect on konjac gum, can hydrolyze guar gum and has a wide acting temperature and pH range, the enzyme activity is kept at 95% or above within the range of 45-75 DEG C, the enzyme activity is kept at 65% or above within the range of pH 5.5-8.5, and various common metal ions can promote the enzyme activity or have little influence on the enzyme activity. The enzyme is beta-mannase with endo-activity, and has a good application prospect in the aspects of feed processing, mannan oligosaccharide preparation, biological polishing, agricultural waste utilization, plant active substance extraction and the like under medium and high temperature conditions.

The invention discloses a synthetic method and application of antrodia camphorata galactomannan oligosaccharide and polysaccharide, and belongs to the field of carbohydrate chemistry and medicinal chemistry. According to the method, five tetrasaccharide building blocks are used as synthons, the construction efficiency of antrodia camphorata galactose 1, 2-cis-glucosidic bonds is improved through remote participation of galactose 4-position benzoyl, and octasaccharide, hexadecanose and tetracosanose of antrodia camphorata are stereoselectively constructed through [4 + 4], [8 + 8] and [16 + 8] glycosylation strategies by utilizing the method; the obtained oligosaccharides and polysaccharides are subjected to deprotection to obtain six antrodia camphorata galactomannan oligosaccharides and polysaccharides with definite structures, single components and anti-inflammatory activity. A cellular immune experiment shows that the oligosaccharide and the polysaccharide can inhibit LPS (lipopolysaccharide)-induced mouse macrophage RAW 264.7 inflammatory response, and have potential application in anti-inflammatory drugs; meanwhile, the antigens are good antigens of antrodia camphorata sugar vaccines and can be developed into sugar vaccines or sugar drugs.

The invention discloses a cactus external preparation and a preparation method thereof, and relates to the technical field of skin external preparations and preparation thereof.The cactus external preparation comprises, by mass, 4.5% of a cactus oligosaccharide compound, the molar ratio of mannan oligosaccharide to arabinogalactan in the cactus oligosaccharide compound is 3: 2, and the cactus oligosaccharide compound contains beta-1, 4 glucosidic bond modification groups; 6% of sericin temperature-sensitive microspheres, the particle size of the microspheres being 200-300 nm, and the phase transition temperature being 32-35 DEG C; according to the invention, staphylococcus epidermidis is activated to secrete antibacterial substances in a targeted manner through the cactus oligosaccharide compound, a physical barrier is formed in combination with the sericin temperature-sensitive microspheres, lactobacillus extracellular vesicles inhibit metabolism of propionibacterium acnes, glycerol caprylate selectively interferes with the fluidity of a pathogenic bacterium membrane, and konjac glucomannan regulates immunity; and a flora regulation and control-barrier protection-immune regulation system is constructed under the assistance of a pulsed electric field pretreatment process and the like, so that the problems of flora imbalance and drug resistance caused by traditional sterilization are solved, and skin micro-ecological balance and inflammation blocking are realized.

The invention discloses preparation of mannan oligosaccharide enzymatic hydrolysate and a method for preparing mannan oligosaccharide from the enzymatic hydrolysate, and relates to the technical field of mannan oligosaccharide preparation. An enzymatic hydrolysate preparation step: preparing a raw material solution and a strain solution; configuring a fermentation tank, a reflux tank and a reflux box, and performing circulating liquid-permeable fermentation; an enzyme concentration detector is arranged in the reflux box, and an enzyme solution is recycled through an enzyme recycling channel; liquid discharge pipes are arranged at the bottoms of the fermentation tank and the return tank, so that fermentation liquid at the bottom of the fermentation tank is discharged, and equivalent raw material liquid is supplemented in time. The method for preparing mannan oligosaccharide comprises the following steps: introducing enzymatic hydrolysate into mannan oligosaccharide enzymolysis equipment arranged in a row, wherein the equipment comprises an enzymolysis tank, a second reflux tank and a second reflux box; preparing a mannan raw material solution; the liquid level in the enzymolysis tank is higher than that in the second reflux tank; a mannan oligosaccharide concentration detector is arranged in the second backflow box; and purifying the recovered mannan oligosaccharide solution. The method solves the problems that mannan oligosaccharide can be obtained only through multiple procedures in the prior art, the overall preparation cost is high, and the continuous degree is low.

The present application provides a mannose oligosaccharide fertilizer synergist with biological synergistic function and a high utilization rate fertilizer, the mannose oligosaccharide fertilizer synergist comprises: phosphorylated modified mannose oligosaccharide; the phosphorylated modified mannose oligosaccharide is obtained by phosphorylating mannose oligosaccharide with a phosphorylating agent.The present application provides a mannose oligosaccharide fertilizer synergist with biological synergistic function and a high utilization rate fertilizer, by obtaining phosphorylated modified mannose oligosaccharide through phosphorylation reaction of mannose oligosaccharide as a fertilizer synergist, compared with mannose oligosaccharide or other saccharides, the affinity with biological macromolecules such as membrane, enzyme and protein is greatly improved, biological synergistic effect is realized, and the absorption of nitrogen, phosphorus and potassium is promoted, and the fertilizer utilization rate is improved.

The invention relates to a compound enzyme preparation for inhibiting generation of zearalenone as well as a preparation method and application of the compound enzyme preparation, and belongs to the technical field of microorganisms and food safety. The prepared compound enzyme preparation contains the medium-temperature alpha-amylase, the glucose oxidase, the mannan oligosaccharide and the soluble starch, the medium-temperature alpha-amylase can effectively inhibit generation of zearalenone in the peanut meal, the glucose oxidase consumes energy to generate acid and inhibits growth of mould, the mannan oligosaccharide can efficiently adsorb the generated zearalenone, and the soluble starch can effectively inhibit growth of the mould. Soluble starch is a stable carrier and can be subjected to enzymolysis through medium-temperature alpha-amylase to generate glucose, and a slow-release substrate is provided for glucose oxidase. The compound enzyme preparation product can fundamentally inhibit the generation of mycotoxin in crop feed raw materials, has small influence on the product quality, is more environment-friendly, and can be widely used, and the raw materials of the compound enzyme preparation are easy to obtain and low in cost.

This invention belongs to the field of seahorse farming technology and discloses a feed additive that promotes the growth of striped seahorses and its application. Specifically, it discloses a feed additive comprising carotenoids, sea squirt powder, oyster powder, mannan oligosaccharides, probiotics, and vitamins. This invention provides a feed additive containing carotenoids, sea squirt powder, oyster powder, mannan oligosaccharides, probiotics, and vitamins. Adding this feed additive to seahorse feed can significantly promote seahorse growth (significantly increasing weight gain rate, specific growth rate, and body length growth rate) and improve seahorse survival rate. This feed additive not only helps improve the economic benefits of seahorse farming but also provides strong technical support for the sustainable development of the seahorse farming industry.

The application discloses a synthesis method and application of Ganoderma annularis galactomannan oligosaccharide and polysaccharide, and belongs to the field of sugar chemistry and medicinal chemistry. Five tetrasaccharide building blocks are used as synthesis subunits, the construction efficiency of Ganoderma annularis galactose 1,2-cis glycosidic bond is improved through remote participation of benzoyl at the 4th position of galactose, and through [4+4], [8+8] and [16+8] glycosylation strategies, Ganoderma annularis octasaccharide, hexadecasaccharide and twenty-four saccharide are stereoselectively constructed; the obtained oligosaccharide and polysaccharide are deprotected to obtain six Ganoderma annularis galactomannan oligosaccharides and polysaccharides with anti-inflammatory activity, which have definite structures and single components. Through cell immunity experiments, it is found that the above oligosaccharide and polysaccharide can inhibit the inflammatory reaction of LPS-induced mouse macrophage RAW 264.7, has potential application in anti-inflammatory drugs, and is also a good antigen of Ganoderma annularis sugar vaccine, and can be developed into a sugar vaccine or a sugar drug.

The invention discloses a macleaya cordata recovery prescription medicine for preventing and treating fish nocardia disease as well as a preparation method and application of the macleaya cordata recovery prescription medicine. The compound medicine consists of 10 to 30 percent of macleaya cordata alkaloid hydrochloride compound, 5 to 15 percent of chitosan quaternary ammonium salt, 10 to 20 percent of mannan oligosaccharide and a medicinal carrier. The preparation method comprises the following steps: carrying out freezing and heat shock pretreatment on a macleaya cordata raw material, and carrying out ultrasonic-flash synergistic extraction by adopting acidified ethanol; performing concentration, macroporous resin purification and high-speed counter-current chromatography separation on the extracting solution to obtain a high-purity macleaya cordata alkaloid hydrochloride compound; and finally, mixing the compound with chitosan quaternary ammonium salt and a carrier, granulating, and carrying out enteric coating. According to the method, active alkaloids such as sanguinarine and chelerythrine in macleaya cordata can be efficiently extracted and refined. According to the compound medicine, through scientific compatibility, for example, macleaya cordata alkaloid is antibacterial, chitosan quaternary ammonium salt and mannan oligosaccharide are used for enhancing immunity and targeting, the bioavailability is improved by utilizing a modern preparation technology, and the compound medicine has a good effect of preventing and treating fish nocardia disease.

The application discloses a kind of composition and its preparation method for promoting mandarin fish feeding and immunity, belong to aquatic feed additive technical field.The composition includes the following weight parts of component: 5-15 parts of mannose oligosaccharide, 1-5 parts of garlicin, 0.5-3 parts of astaxanthin powder, 2-8 parts of glucose oxidase, 10-25 parts of plant fermentation extract;Plant fermentation extract is with perilla seed as raw material, after deionized water leaching, residue material is extracted, inoculates lactobacillus reuteri fermentation, and then is dried, and is prepared by crushing powder.The application is synergized by mannose oligosaccharide, garlicin, astaxanthin powder, glucose oxidase and perilla seed fermentation extract prepared by specific process, improves mandarin fish feeding amount from two paths of direct feeding attraction and indirect feeding promotion, strengthens body disease resistance from two levels of intestinal local defense and systemic immune activation, and can effectively solve the problem of low feeding rate and weak disease resistance of mandarin fish during domestication and material conversion period.

The invention relates to the field of biopesticides, in particular to application of mannan oligosaccharide (MOS) in prevention and treatment of tobacco black shank and root rot. As an inducer, mannan oligosaccharide can induce the resistance of plants to the infection of related pathogenic bacteria. The induced defensive reaction not only is enhanced at the initially infected part of the plant, but also is enhanced at the non-infected and spatially isolated part, so that the plant obtains systematic resistance.

The invention discloses a traditional Chinese medicine composition for preventing and treating chicken-derived anatipestilence disease and application of the traditional Chinese medicine composition, and belongs to the technical field of animal medicine. The composition is prepared from 10 to 20 parts of radix isatidis, 8 to 15 parts of radix scutellariae, 10 to 20 parts of herba violae, 5 to 10 parts of herba hedyotidis diffusae, 15 to 25 parts of semen coicis, 8 to 15 parts of semen lablab album, 5 to 10 parts of radix platycodonis, 4 to 8 parts of radix puerariae, 4 to 8 parts of radix paeoniae rubra, 8 to 15 parts of radix codonopsis, 3 to 8 parts of liquorice root, 3 to 8 parts of rhizoma zingiberis recens, 6 to 10 parts of beta-glucan and 4 to 8 parts of mannan oligosaccharide. The preparation method comprises the steps of material selection, decoction, concentration, membrane separation and packaging. Experiments show that the traditional Chinese medicine composition provided by the invention can effectively inhibit riemerella anatipestifer and can effectively relieve the symptoms of chicken-derived riemerella anatipestifer. Therefore, the drug resistance problem of riemerella anatipestifer is solved, the green breeding requirement is met, and the method is suitable for large-scale application.

The application discloses a preparation method of konjak vinegar, which comprises the following steps: firstly, konjak powder is subjected to enzymolysis and saccharification by using a glucanase; then, alcohol fermentation and acetic acid fermentation are sequentially performed; meanwhile, purified konjak powder is supplemented before the acetic acid fermentation to perform deep fermentation; finally, konjak vinegar is prepared by using a fructose-glucose syrup to adjust the flavor. The process disclosed by the application has higher production efficiency, the prepared product has low trimethylamine acetate content and high safety, and compared with traditional fruit vinegar, the prepared konjak vinegar has slightly thick taste and contains more functional components such as mannose oligosaccharide, has high acceptance and can play a certain disease prevention effect, and has wide application prospect.

The present application provides a high-temperature mannanase and its application, and the amino acid sequence thereof is SEQ ID NO:1. The mannanase of the present application has the strongest hydrolysis effect on konjac gum, and can also hydrolyze guar gum, has a wide temperature and pH range, the enzyme activity is maintained at 95% or above in the range of 45-75 DEG C, the enzyme activity is maintained at 65% or above in the range of pH 5.5-8.5, and various common metal ions can promote the enzyme activity or have little effect on the activity. The enzyme is a beta-mannanase with endo-activity, and has a good application prospect in feed processing, preparation of manno-oligosaccharides, biological polishing, utilization of agricultural waste, extraction of plant active substances and the like under medium-high temperature conditions.