Complex enzyme hydrolysis guar gum preparation soluble diet fiber and manna oligose method

A compound enzyme hydrolysis and guar gum technology, which is applied in the field of food processing, can solve the problems of few reports on the hydrolysis of guar gum, and achieve the effect of easy separation and high conversion rate

Active Publication Date: 2017-02-22
BEIJING GUAR SCI&TRADING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are few reports on the hydrolysis of guar gum. Among them, the Chinese invention patent application with the application number 201510174562.2 discloses a compound degradation of guar gum by using three enzymes of β-mannanase, xylanase and endoglucanase. A method for preparing galactomannose from Erdou gum; the Chinese invention patent application with the application number 201510175963.X discloses a method of compoundly degrading melon by using three enzymes of β-mannanase, xylanase and cellulase A method for preparing galactomannose from guar gum; the Chinese invention patent application with the application number 201510175978.6 discloses a method for degrading guar by using β-mannanase, endoglucanase and cellobiase The method for preparing galactomannose oligosaccharides from bean gum. The above invention patents disclose the preparation of galactomannose oligosaccharides, which do not involve the preparation of soluble dietary fiber. At present, there is no relevant information about the preparation of soluble dietary fiber using guar gum. report

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  • Complex enzyme hydrolysis guar gum preparation soluble diet fiber and manna oligose method
  • Complex enzyme hydrolysis guar gum preparation soluble diet fiber and manna oligose method
  • Complex enzyme hydrolysis guar gum preparation soluble diet fiber and manna oligose method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0052] Embodiment 2 Mannanase compound hydrolysis guar gum of different addition amount

[0053] Weigh 5g of guar gum and dissolve it completely in 100mL of distilled water (distilled water can also be replaced with phosphate buffer or citric acid buffer with a pH of 7.0, etc.), according to the ratio of guar gum to 100, 200, 400, 800 , 1000 and 2000 U / g were added with mannanase, placed at 50°C for hydrolysis for 8 hours, and after enzymolysis, inactivated in a boiling water bath for 10 minutes to obtain an enzymolysis solution. After the obtained enzymolysis solution was centrifuged at 10000rpm for 10min, the supernatant, that is, the crude sugar solution was collected, and its viscosity was measured with a DV-1 rotational viscometer at 25°C, and the crude sugar solution was determined by the 3,5-dinitrosalicylic acid method. The content of reducing sugar in the medium and calculate the yield of reducing sugar. The experimental results are shown in Table 1.

[0054] Table ...

Embodiment 3

[0057] Example 3 Composite Hydrolysis of Guar Gum with Different Additions of α-Galactosidase and Mannanase

[0058] Weigh 5g guar gum and dissolve it in 100mL distilled water (distilled water can also be changed to pH 7.0 phosphate buffer or citrate buffer, etc.), add mannanase according to the ratio of guar gum 1000U / g, According to the ratio of 0, 100, 200, and 400 U / g to guar gum, galactosidase was added respectively, placed at 50 ° C for 8 hours, and after enzymolysis, the boiling water bath was inactivated for 10 minutes to obtain an enzymolysis solution. After the obtained enzymolysis solution was centrifuged at 10000rpm for 10min, the supernatant, that is, the crude sugar solution was collected, and its viscosity was measured with a DV-1 rotational viscometer at 25°C, and the crude sugar solution was determined by the 3,5-dinitrosalicylic acid method. The content of reducing sugar in the medium and calculate the yield of reducing sugar.

[0059] Table 2 shows the visc...

Embodiment 4

[0062] Example 4 Experimental results of different hydrolysis time compound enzyme hydrolysis guar gum

[0063] Weigh 5g guar gum and dissolve it in 100mL distilled water, add mannanase 1000U / g and galactosidase 200U / g according to the ratio of guar gum, and place them at 50°C to hydrolyze 1, 2, 4 , 8, 12, and 24 hours, after enzymolysis, inactivate in a boiling water bath for 10 minutes to obtain an enzymolysis solution, centrifuge the obtained enzymolysis solution at 10,000 rpm for 10 minutes, collect the supernatant, and obtain the crude sugar solution. The viscosity of the crude sugar solution was measured with a DV-1 rotational viscometer at 25°C, and the reducing sugar content in the crude sugar solution was determined by the 3,5-dinitrosalicylic acid method and the yield of reducing sugar was calculated.

[0064] The viscosity of crude sugar solution and the yield of reducing sugar obtained by hydrolyzing guar gum with compound enzymes at different hydrolysis times are ...

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Abstract

The invention discloses a complex enzyme hydrolysis guar gum preparation soluble diet fiber and manna oligose method, comprising the steps of mixing guar gum solution, manna glycanase and galactose neuraminidase, hydrolyzing, filtering, decolorizing, ion exchanging and condensing; conducting anhydrous ethanol precipitation and desiccation. The method provides a source for soluble diet fiber; utilizing the preparation method, can prepare a middle product of a gala containing manna oligose soluble diet fiber syrup, the weighted average molecular weight of the syrup is about 13700Da; the hydrolysis rate and the conversion rate of gala containing manna glycanase are high, the product can be easily isolated, the weighted average molecular weight of the prepared soluble dietary fiber is about 14600Da, the polymerization degree of the manna oligose is between 2-5.

Description

technical field [0001] The invention belongs to the field of food processing, and in particular relates to a method for preparing soluble dietary fiber and mannan oligosaccharides by using compound enzymes to hydrolyze guar gum. Background technique [0002] Galactomannan generally consists of a mannan main chain formed by β-1,4-glycosidic bonds and a galactose residue side chain formed by α-1,6-glycosidic bonds, and is widely found in various plant cell walls and In the endosperm (Thombare et al. International Journal of Biological Macromolecules, 2016, 88:361-372). Guar gum is a natural vegetable gum derived from guar beans (Cyamopsis tetragonolobus). It has many excellent properties such as high viscosity, good water solubility, acid and alkali resistance, and high temperature resistance. It is widely used in various industries. Its main component is galactomannan (Thombare et al. International Journal of Biological Macromolecules, 2016, 88:361-372). In galactomannan fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/26A23L29/30C12P19/04C12P19/12
CPCA23V2002/00C12P19/04C12P19/12A23V2200/30A23V2250/28A23V2250/5116A23V2300/28
Inventor 闫巧娟袁江宏李延啸刘燕静江正强张伟
Owner BEIJING GUAR SCI&TRADING
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