Yeast cell wall and preparation method thereof, forage additive and preparation method thereof, and forage

A yeast cell wall and feed additive technology, applied in the field of yeast cell wall and preparation, to achieve the effect of improving litter rate, feasible process, and effective and feasible product application

Active Publication Date: 2013-08-14
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0025] At present, there is no additive product that can be specially used to improve the reproductive performance of sows. In terms of improving the reproductive performance of sows, there is no one that can not only solve the poisoning of fee...

Method used

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  • Yeast cell wall and preparation method thereof, forage additive and preparation method thereof, and forage
  • Yeast cell wall and preparation method thereof, forage additive and preparation method thereof, and forage
  • Yeast cell wall and preparation method thereof, forage additive and preparation method thereof, and forage

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preparation example Construction

[0065] The preparation method of sodium-modified montmorillonite of the present invention is as follows: sodium polyphosphate and sodium carbonate are added in montmorillonite slurry, and described montmorillonite slurry is by dissolving natural montmorillonite in water, preferably every The volume of water added to one kilogram of natural montmorillonite is 1-1.5 liters, and the prepared montmorillonite slurry is stirred evenly, ground, aged to obtain a sodium montmorillonite slurry, and then mixed with an aluminum crosslinking agent, stirred, and hydrolyzed , that is. The mixing ratio of the sodium-modified montmorillonite slurry and the aluminum cross-linking agent described therein is 5-10mmol / g (with AlCl 3 molar number and natural montmorillonite mass), the preferred mixing ratio is 6mmol / g; wherein the addition of sodium polyphosphate is 5-10% of the natural montmorillonite addition, preferably 8%, the addition of sodium carbonate The amount is 0.5-1.0% of the added am...

Embodiment 1

[0081] The preparation of embodiment 1 yeast cell wall

[0082] First, the yeast is formulated into 20kg of yeast milk according to the ratio of dry matter to 10% by mass (mass ratio of yeast dry matter to water: 1:9), then boiled at 89°C for 1 hour, then cooled to 65°C, and added according to the dry matter of yeast Add papain at a ratio of 2‰, adjust the pH to 5.5, and act for 6 hours; then adjust the pH to 5.0, the temperature is 68°C, add nuclease at a ratio of 1.0‰ of yeast dry matter, and act for 8 hours, then Lower the temperature to 50°C, adjust the pH to 5.6, add bacterial protease according to the proportion of 0.2‰ of yeast dry matter, and act for 5 hours; then inactivate the enzyme at 85°C for 30 minutes, and finally centrifuge, take the heavy phase after centrifugation, and carry out The yeast cell wall A1 was obtained by powder spraying and drying, the mass content of β-yeast glucan was determined to be 20%, and the mass content of mannan oligosaccharide was 60%....

Embodiment 2

[0083] The preparation of embodiment 2 yeast cell wall

[0084] Firstly, the yeast is formulated into 20kg of yeast milk according to the ratio of dry matter to 10%, and then boiled at 93°C for 0.5 hours, then cooled to 63°C, and papain is added according to the ratio of yeast dry matter to 1‰, and the pH is adjusted to 5.7. Act for 5 hours; then adjust the pH to 5.3, the temperature is 68°C, add nuclease according to the ratio of 0.5‰ of yeast dry matter, after 9 hours of action, then lower the temperature to 48°C, adjust the pH to 5.4, and add according to the amount of yeast dry matter Add bacterial protease at a ratio of 0.3‰, and act for 4 hours; then inactivate the enzyme at 80°C for 50 minutes, and finally centrifuge, take the heavy phase after centrifugation, and dry it by powder spraying to obtain yeast cell wall A2. The mass content of polysaccharide is 25%, and the mass content of manno-oligosaccharide is 70%.

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Abstract

The invention relates to a yeast cell wall, comprising 20%-70% by mass of beta-yeast glucan and 20%-70% by mass of mannan oligosaccharide. The invention also relates to a forage additive, comprising the yeast cell wall, sodium modified montmorillonite and selenium yeast, and by mass, a ratio of the yeast cell wall to the sodium modified montmorillonite is 1 : 9-9 : 1; and a ratio of a total of the yeast cell wall and the sodium modified montmorillonite to the selenium yeast is 10 : 1-20 : 1. The invention also relates to a preparation method for the yeast cell wall. The preparation method comprises the steps of breaking the cell wall of live yeast through self-dissolution, degrading the yeast with a compound enzyme, separating, purifying, drying and deactivating the enzyme to obtain the yeast cell wall, wherein the compound enzyme comprises papain, nuclease and bacteria protease, and by mass, the addition amount of the papain, nuclease and bacteria protease is 0.1-0.3%, 0.05-0.2% and 0.01-0.03% that of the dry material of the yeast. The invention also relates to forage, an addition level of the additive in the forage is 1.5-2.5 g/kg.

Description

technical field [0001] The present invention relates to a yeast cell wall and a preparation method thereof, and a feed additive containing the yeast cell wall and a preparation method thereof, in particular to a feed additive containing yeast cell walls, sodium-modified montmorillonite and yeast selenium and a preparation method thereof; It relates to a feed containing the above-mentioned feed additive. Background technique [0002] The level of pig production efficiency and economic benefits depends to a large extent on the reproductive efficiency of sows, and the level of reproductive efficiency of sows depends on factors such as genetics, environment, disease, feeding management and nutritional level. In the modern pig industry, the reproductive performance of sows directly affects the efficiency of pig farms. In order to give full play to the maximum production performance of sows, people have taken various measures, but at the same time it has also brought about the pr...

Claims

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Application Information

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IPC IPC(8): C12N1/06A23K1/16A23K1/175
Inventor 朱金林俞学锋李知洪余明华姚鹃
Owner ANGELYEAST CO LTD
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