Production method of konjak mannose using cellulase

A technology of cellulase and mannan oligosaccharides, which is applied in the field of microbial enzymatic hydrolysis engineering, can solve the problems of too large molecular chain, which is not conducive to the activation and improvement of animal immunity and the improvement of human micro-ecological environment, and achieves simple enzymatic hydrolysis process, The effect of low cost and high efficiency

Active Publication Date: 2007-01-31
WUHAN SUNHY BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0016] Its shortcoming is that the number of oligosaccharide molecules prepared by this process condition is about 28, and the molecular chain is too large, which is not co...

Method used

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  • Production method of konjak mannose using cellulase
  • Production method of konjak mannose using cellulase
  • Production method of konjak mannose using cellulase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1) Enzymatic hydrolysis:

[0045]In 1000 milliliters of distilled water, add 125 grams of konjac powder, stir evenly, adjust its temperature to 45 ° C, and adjust the acidity and alkalinity to pH 5 with 0.5M (molar concentration) NaOH (sodium hydroxide) solution. A mixture of cellulase and hemicellulase (enzyme activity ratio: 3:1) with a total enzyme activity of 12500 u (international units) was added, and the mixture was stirred and reacted at 45° C. and pH 5 for 7 hours. During the reaction, the pH was maintained at 5 by adjusting with 0.5M NaOH solution. After the reaction was finished, inactivate it in a boiling water bath for 15 minutes, and let it stand overnight at room temperature.

[0046] 2) Separation coupling purification:

[0047] The product of the previous step was centrifuged at 3600 rpm for 10 minutes to remove the precipitate. After drying, the precipitate can be used as feed, and can also be put into enzymatic hydrolysis again. The centrifuged mo...

Embodiment 2

[0051] 1) Enzymatic hydrolysis:

[0052] In 1000 milliliters of distilled water, add 110g konjac fine powder, 15 gram centrifugal separation filter residues, 3 gram ultrafiltration membrane separation filter residues, stir evenly, add cellulase and hemicellulase mixture (cellulase enzyme activity: hemicellulase Enzyme activity=3:1, total enzyme activity 12500u), fully mix, adjust its temperature to 50°C, and adjust pH to 5 with 0.5M (molar concentration) sodium hydroxide (NaOH) solution, at 50°C, pH5 The reaction was stirred for 7 hours under certain conditions, and the enzymatic hydrolysis reaction was terminated. During the reaction, the pH was maintained at 5 with 0.5M NaOH solution. After the reaction was terminated, it was inactivated in a boiling water bath for 15 minutes, and left standing overnight at room temperature.

[0053] 2) Separation coupling purification:

[0054] The product of the previous step was centrifuged at 3600 rpm for 10 minutes to remove the prec...

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PUM

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Abstract

The present invention relates to a production method for preparing konjak mannoligose by using cellulase. Its technological process includes the following several procedures: enzymolysis, filtraction, decoloring, concentration and drying. It is characterized by that said invention adopts non-specific commercial enzyme (including cellulose and hemicellulase), and utilizes centrifugal filtering and membrane filtering combined process, so that said invention can obtain high-purity konjak mannoligose. Besides, said invention also provides the concrete requirements and steps of the above-mentioned every procedure.

Description

technical field [0001] The invention relates to microbial enzymatic hydrolysis engineering, which belongs to the production technology of functional oligosaccharide enzymatic hydrolysis. technical background [0002] Oligosaccharides (oligosaccharides) are a general term for small polymers formed by 2-10 monosaccharides connected by glycosidic bonds at certain positions. Mannan oligosaccharides (or glucomannan oligosaccharides) are the enzymatic hydrolyzates of mannan (glucomannan), which are widely found in konjac, guar gum, kale gum, and various microbial cell walls. Mannan oligosaccharides, as a new type of microecological effect additive, have dual functions of adsorbing intestinal pathogenic bacteria and exerting immunomodulatory effects, and are one of the most promising alternatives to antibiotics. At the same time, the product has a wide range of uses in food, medicine and other industries. [0003] 1. For feed additives, the domestic ...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12P19/04A23K1/16A23L1/30A61K31/715A61K31/702A61P37/04A61P35/00A61P9/12A61P9/00A61P3/10A61P3/06A61P1/10A23K10/14A23K20/163A23L33/10
Inventor 詹志春
Owner WUHAN SUNHY BIOLOGICAL
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