Earthworm source amino acid water-soluble fertilizer and use method

Abstract

The invention relates to the field of waste utilization of earthworm processing, in particular to an earthworm source amino acid water-soluble fertilizer and a use method. The method comprises the following steps that in the extraction process of eisenia fetida kinase, residues filtered in the preparation process of crude extract serve as a first raw material, and the first raw material is remaining substances except supernate; the raw material II is a substance filtered by a membrane of an ammonium sulfate-membrane separation extraction method; the raw material I and the raw material II are mixed according to the volume ratio of 3: 1, and then an inorganic nitrogen source and an organic amino acid precursor are obtained, so that basic conditions of the water-soluble fertilizer are met. The process combination is creatively utilized, and no waste is generated. And moreover, no polluted organic solvent is used.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of waste utilization of earthworm processing, in particular to earthworm source amino acid water-soluble fertilizer and use method. BACKGROUND

[0002] On April 20, 2025, Zhang Hongyan, Yin Hong, Han Shanshan, Zhang Qiang, and Xindonglin published "Study on Extraction Process and Enzymatic Properties of Eisenia foetida Kinase" in Acta Agronomica. The process of the present application is closely connected with the process.

[0003] In the process, a large amount of waste and liquid is produced during the processing of Eisenia foetida.

[0004] These things are wasted and not fully utilized.

[0005] On February 25, 2025, with "(AB=(earthworm and water-soluble fertilizer))" as the abstract keyword, and checking the synonym expansion, a search was conducted in the China Patent Publication Database, and the following documents were found.

[0006] CN107892636A provides a multi-nutrient macroelement water-soluble fertilizer powder product, which comprises the following components: urea, urea phosphate, polyphosphate, potassium nitrate, potassium dihydrogen phosphate, calcium nitrate, magnesium sulfate, water-soluble trace element component, earthworm manure, composite chelating agent, growth regulator, soil loosening agent, and carboxymethyl cellulose branch N-isopropyl acrylamide copolymer; the water-soluble trace element component includes two or more of iron, copper, zinc, boron, manganese, and molybdenum compounds; the composite chelating agent is a combination of ethylenediaminetetraacetic acid disodium salt and biodegradable ethylenediamine disuccinic acid; the growth regulator is a combination of one or any proportion of diethylaminoethanol hexanoate, alpha-naphthylacetic acid, or indoleacetic acid. The water-soluble fertilizer powder product has simple preparation method and low cost. The fertilizer product produced by the present application has high fertilizer efficiency, balanced nutrient composition, good water solubility, environmental protection, no pollution, reasonable component ratio, and is not easy to caking during long-term storage.

[0007] CN107382434A provides a preparation method of a sugar alcohol containing high phosphorus type water-soluble fertilizer, and the method comprises the following steps: weighing water, phosphorus source, nitrogen source, potassium source, sugar alcohol liquid, sodium octaborate tetrahydrate, calcium nitrate, borax, ferrous sulfate, vinasse, wood ash, pig bone powder, earthworm manure, pumpkin vine and folic acid; the method can improve the stress resistance of crops by 60%-80%, and has the characteristics that the potassium source and other components are used in combination to form stable small molecule chelates which are easily absorbed by crops, so that the synergistic effect is generated, cell division is participated, the fruit setting rate is improved, and the plant root growth is promoted, thereby improving the stress resistance and the ability to adapt to the external environment of the plant; the method can improve the stress resistance of the plant, participate in the osmotic regulation in the plant cells, and make the plant adapt to the stress growth by regulating the cell permeability; the method can improve the resistance to active oxygen, and avoid the active oxygen damage of the plant caused by reasons such as sunburn, drought, disease and oxygen deficiency.

[0008] CN105967761A provides a method for producing amino acid water-soluble fertilizer by using Japanese earthworms, which comprises the following steps: A. hydrolyzing complex amino acid: after extracting earthworm essence, the remaining earthworm crude protein liquid is added with alkaline protease liquid for enzymolysis, and acid protease is further added for hydrolysis to obtain hydrolyzed complex amino acid; B. chelation reaction: ZnSO.7HO and NaSeO are added into the hydrolyzed complex amino acid for chelation reaction to obtain complex amino acid chelate; C. separation and purification: the complex amino acid chelate is separated and purified by using an organic solvent such as methanol or ethanol, and then dried to obtain amino acid water-soluble fertilizer. The amino acid water-soluble fertilizer has the advantages that the complex amino acid and trace elements are chelated to form water-soluble fertilizer which can be quickly absorbed by plants, and the water-soluble fertilizer has the characteristics of yield increase, improvement of fruit quality, stress resistance, soil improvement and improvement of drug utilization rate.

[0009] CN105993261B discloses a soil improvement method based on calcium-magnesium-silicon-potassium bioactive fertilizer. After soil ploughing, organic water-soluble fertilizer is applied, and the mixture is stacked in situ in the field during slack season, the mixture is stacked to a height of about 30-100 cm, and a layer of straw is covered on the top to block light. Soil conditioner and soil modifier are applied, earthworms are inoculated, calcium-magnesium-silicon-potassium bioactive fertilizer is applied, and the mixture is stirred and mixed after ploughing once. The mixture is applied as fertilizer in the field after ditching, and the soil is ploughed once. The fertilizer has long effect, the stack, the soil conditioner and the soil modifier can improve soil fertility for a long time, the organic carbon and the bio-fermented organic carbon filtrate can provide carbon nutrition for microorganisms in the soil, increase the diversity of microorganisms in the soil, and the activated calcium-magnesium-silicon-potassium bioactive fertilizer can improve the utilization rate of trace elements by crops, and the chelated trace elements have high availability and are easily absorbed by crops.

[0010] CN118271136A belongs to the technical field of ecological fertilizer, and discloses an organic water-soluble fertilizer containing amino acids, which comprises, in percentage by mass, 75-90% of earthworm manure extract, 7-20% of amino acid material, 0.1-0.5% of hematite powder, and 2-5% of sodium tetraborate pentahydrate. The number of the microbial flora in the earthworm manure extract is 20-60 million per milliliter. The large amount of enzymes produced by the microbial flora in the earthworm manure extract is used to decompose and transform trace elements in the amino acid water-soluble fertilizer applied to the soil, so that a large amount of trace elements are dissociated in the soil in the form of ions, which is beneficial to the absorption of the roots of crops and improves the fertilizer efficiency, so that the plants can absorb the fertilizer well, the transformation is fast, the effect is remarkable, the development of the roots of crops is promoted, and thus the rapid growth of crops is promoted.

[0011] CN118901520A discloses an organic golden wheat and a planting method thereof, and particularly relates to the technical field of agriculture. The organic golden wheat is prepared by using organic sheep manure and pepper residue as base fertilizer, using probiotic Chinese herbal medicine water-soluble fertilizer as fertilizer, using Chinese herbal medicine water-soluble pesticide as insecticide, and using wheat seeds. The probiotic Chinese herbal medicine water-soluble fertilizer is prepared by using earthworm enzyme as a basic fermentation enzyme accounting for 5-10% of the total weight, using water as a fermentation and dilution medium accounting for 85-90% of the total weight, and using Chinese herbal medicine accounting for 5-10% of the total weight. The Chinese herbal medicine includes radix astragali, radix codonopsis, lycium barbarum, angelica sinensis, goldthread, radix polygoni multiflori, poria cocos and pericarpium citri reticulatae. The organic golden wheat has high protein content, high flavonoid and lutein content, no pesticide residue, and the noodles made of the organic golden wheat have a unique strong wheat flavor and a long aftertaste.

[0012] CN109776120A provides a kind of fertilizer and its application, and the fertilizer includes biological organic fertilizer and organic water-soluble fertilizer, and the biological organic fertilizer is prepared by mixing livestock and poultry manure, mushroom residue, crop straw, rapeseed meal, earthworm manure, superphosphate, carbon method filter mud, adding fermentation bacteria to carry out initial fermentation, adding compound functional bacteria to carry out composting fermentation; compound functional bacteria agent includes bacillus pumilus, bacillus megaterium and trichoderma harzianum; organic water-soluble fertilizer is prepared by mixing fruit and vegetable waste, corn germ meal, sesame meal, sugarcane molasses, waste beer fermentation liquor and water to carry out natural fermentation, then adding inorganic compound fertilizer and glucosan to concentrate and prepare. The fertilizer of the application can significantly improve the soluble solids, sugar-acid ratio, vitamin C content and hardness of strawberries, and can be applied to the planting of strawberries as a fertilizer for strawberries.

[0013] CN108329157A discloses a nutritionally balanced instant water-soluble fertilizer powder, which is made from the following raw materials: straw 100-150, water-soluble trace elements 20-30, chelating agent 2-3, earthworm manure 40-60, bamboo charcoal powder 10-20, complex microbial population 6-9, lignin peroxide enzyme and cellulase in appropriate amounts; the present application adopts ultrasonic enzymolysis of straw, which greatly shortens the enzymolysis time while improving the enzymolysis rate, adopts freeze drying and vacuum drying to maximize the retention of active substances and nutrient components in the raw materials that are beneficial to crops, and adopts superfine crushing to make the fertilizer particle size small, the solubility high, and the dissolution speed fast, which will not block the nozzle and other equipment, and the product fertilizer is easily absorbed by crops, has high crop utilization rate, good stability, can improve soil fertility while enhancing the stress resistance of crops, and can improve the microecological environment of soil, increase the number of microorganisms in soil, and significantly improve the pollution status of existing soil.

[0014] CN111018613A discloses a preparation method of a fruit tree seedling nutrition type humic acid-containing water-soluble fertilizer, comprising the following steps: S1, preparing the following raw materials: humic acid mixture, urea, crop straw, dipotassium hydrogen phosphate, earthworm manure, potassium chloride, magnesium sulfate, ammonia water, traditional Chinese medicine residue and livestock and poultry manure; S2, straw treatment: ①the crop straw is crushed by a crusher and sieved through an 80-100 mesh sieve; ②the sieved crop straw is placed in a sealed box. The fertilizer prepared by the present application not only provides organic matter, nitrogen, phosphorus and potassium, but also improves soil, increases fertilizer efficiency, increases crop yield and improves crop quality; the traditional Chinese medicine residue in the fertilizer plays a synergistic and resistant effect, exerts a drug effect, and has the effects of preventing and repelling insects during the growth of fruit trees, ensuring plant robustness and yield increase; the addition of earthworm manure can improve the aeration, drainage and high water holding capacity of soil.

[0015] CN119390492A provides an earthworm amino acid water-soluble fertilizer, a preparation method and application thereof, the earthworm amino acid water-soluble fertilizer comprises the following components by weight: ferrous sulfate 70-100 parts, boric acid 8-14 parts, potassium molybdate 10-18 parts, potassium dihydrogen phosphate 3-7 parts, manganese sulfate 3-7 parts, zinc sulfate 2-7 parts, copper sulfate 3-7 parts, potassium chloride 2-5 parts and earthworm fermentation liquor 900-1100 parts. The nitrogen fertilizer in the amino acid water-soluble fertilizer exists in the form of amino acid molecules, which is easy to be absorbed and utilized by crops, and can significantly reduce the conversion to inorganic nitrogen form (such as nitrate), effectively reducing the nitrate content of leaf vegetables. In addition, the proportion of phosphorus and potassium in the amino acid water-soluble fertilizer is determined according to the nutrient requirements of leaf vegetables, and comprehensive trace elements are added, which can promote the growth of leaf vegetables and improve the taste of leaf vegetables.

[0016] CN112851431A discloses a tree rejuvenation package, which is composed of amino acid-containing water-soluble fertilizer, earthworm liquid special fertilizer and humic acid-containing water-soluble fertilizer, and the mass ratio of the amino acid-containing water-soluble fertilizer, the earthworm liquid special fertilizer and the humic acid-containing water-soluble fertilizer is 1-15:1-10:10-50; the amino acid-containing water-soluble fertilizer is composed of complex amino acid, magnesium sulfate, pure water and indole acetic acid; the earthworm liquid special fertilizer is composed of 20wt% earthworm liquid, left-handed amino acid, chelated trace elements, magnesium sulfate and pure water; and the humic acid-containing water-soluble fertilizer is composed of mineral humic acid, complex amino acid, urea, ammonium sulfate, monoammonium phosphate and potassium sulfate. The tree rejuvenation package has good water solubility and high absorption efficiency, can provide comprehensive nutrition for trees, has obvious tree rejuvenation effect, and is convenient to use, and can be used for irrigation, root irrigation or foliar spraying.

[0017] CN106495836A discloses a preparation method of an organic water-soluble fertilizer, and belongs to the technical field of fertilizer preparation. First, Aspergillus oryzae is cultured and then centrifuged, and the lower sediment is collected. Then, the sediment is fermented with potato mud, bran, soybean powder and the like, and the filtrate is distilled to obtain polypeptides. The polypeptides are stirred and reacted with succinic anhydride, xanthan gum and the like to obtain a coating agent. The biogas liquid is filtered and fermented with earthworm manure, peanut skin and the like. The fermentation liquid is centrifuged to collect supernatant and lower sediment. The sediment is stirred with straw powder and composted. The supernatant is sprinkled on the compost and turned over. After composting, the mixture is dried and crushed. The obtained mixed powder is granulated with the remaining supernatant and starch. Finally, the coating agent is sprayed on the surface of the granules and dried to obtain the organic water-soluble fertilizer. The water-soluble fertilizer has long fertilizer efficiency, high nutrient absorption efficiency, fast dissolution, is not easy to caking, and does not cause problems such as clogging of the spray head and pipeline.

[0018] CN109076940A discloses a soilless culture medium suitable for strawberries, and relates to the field of plant cultivation. The soilless culture medium is prepared by mixing grass charcoal, perlite and earthworm manure at a volume ratio of 3:1:1. The method for cultivating strawberries using the soilless culture medium comprises the following steps: 1) pretreatment: before planting, the strawberry stolon shoots are left with 2-3 new leaves, the root system is left with about 8 cm, and part of the old roots are cut off; 2) temperature management: the daytime temperature is controlled at 24-28 DEG C, and the nighttime temperature is controlled at 8-12 DEG C; 3) humidity management: the relative humidity in the greenhouse is kept at about 60%; and 4) water and fertilizer management: three types of water-soluble fertilizer are used for irrigation. The application can realize yield increase of strawberries and improve economic benefits by using the soilless culture medium and scientific cultivation methods, and has high practical value.

[0019] CN107879822A provides a chelated trace element water-soluble fertilizer powder product, which comprises the following components: monoammonium phosphate, potassium nitrate, superphosphoric acid calcium, water-soluble trace element component, complex chelating agent, growth regulator, soil loosening agent, earthworm manure, and complex probiotic group. The water-soluble trace element component includes two or more of iron, copper, zinc, boron, manganese, and molybdenum compounds, wherein the content of any combination of two or more of iron, copper, zinc, boron, manganese, and molybdenum is not less than 10%. The complex chelating agent is a mixture of sodium tripolyphosphate, sodium hexametaphosphate, and ethylenediamine disuccinic acid in a molar ratio of 0.5-2:1.5-3:1. The water-soluble fertilizer powder product has simple preparation method and low cost. The fertilizer product produced by the present application has scientific and reasonable component ratio, can activate soil fertility, and provides comprehensive guarantee for the healthy growth of crops. The product has good water solubility, low ecological toxicity, is easily absorbed and utilized by crops, and is easily biodegradable.

[0020] CN107285962A discloses a water-soluble fertilizer containing earthworm extract, which contains 2%-5% of earthworm extract and one or more of fully soluble macroelements, medium elements, trace elements, and humic acid. The invention saves manpower and achieves the purpose of improving soil.

[0021] CN115623894B belongs to the technical field of soil remediation, and discloses a soil improvement method for reducing plant absorption of heavy metal cadmium, wherein earthworm manure and alkali-modified biochar are added into soil and stirred uniformly; after the planted plants grow for 7-10 days, dilute 600 times of alkaline water-soluble fertilizer is sprayed onto the soil, and is applied every 7 days. The present application solves the problem that plants easily absorb heavy metal cadmium in the soil during planting, resulting in excessive heavy metal cadmium content.

[0022] CN106008081A discloses an acidified soil remediation method, wherein soil acidification conditioner is applied to soil at an amount of 25 kg / acre; one week after the application of soil acidification conditioner, a mixture is placed in the field in situ during the off-season, the mixture is stacked to a height of 30-100 cm, and a layer of straw is covered on the top of the stack to block light; the mixture is stacked for 5-10 days, stirred once, and then stacked for another 15-25 days; acidified soil organic water-soluble fertilizer is applied to the soil at an amount of 100 kg / acre; 30 days after the application of acidified soil organic water-soluble fertilizer, earthworms are inoculated, and regular watering is performed to maintain a humidity of 70-90% and a temperature of 30°C or less; the stacked product is stirred and mixed uniformly, and the mixture is applied to the field as fertilizer after ditching; soil acidification conditioner is applied to the soil at an amount of 50 kg / acre. The application of soil acidification conditioner, acidified soil organic water-soluble fertilizer, inoculation of earthworms, and soil acidification conditioner significantly reduces the degree of soil acidification and improves soil fertility.

[0023] CN111018602A provides a preparation method of trace and macroelement composite water-soluble fertilizer. The preparation method of the trace and macroelement composite water-soluble fertilizer includes the following raw materials by mass fraction: water-soluble nitrogen fertilizer 12-16 parts, water-soluble potassium fertilizer 21-24 parts, water-soluble phosphorus fertilizer 17-22 parts, water-soluble trace element mixture 5-9 parts, alginic acid 8-11 parts, poly-enzyme 6-8 parts, chelating agent 6-9 parts, and earthworm manure 10-13 parts. The chelation state of the macroelements and trace elements is good, and each component of the macroelements is fully chelated by ultrasonic oscillation, and each component of the trace elements is fully chelated under the action of stirring, so that the use effect of the fertilizer can be improved when used. The method is simple, and the steps are clear and explicit, so it is suitable as a preparation process for macroelement and trace element composite powder water-soluble fertilizer. The prepared composite powder water-soluble fertilizer contains the macroelements required by plants, as well as iron, molybdenum, manganese, boron, and copper trace elements, so the nutrition of the water-soluble fertilizer is balanced.

[0024] CN105993261A discloses a soil improvement method based on calcium-magnesium-silicon-potassium bioactive fertilizer. After soil plowing, organic water-soluble fertilizer is applied, and the mixture is stacked in situ in the field during the slack season. The mixture is stacked to a height of about 30-100 cm, and a layer of straw is covered on the top to block light. Soil conditioner and soil modifier are applied, earthworms are inoculated, calcium-magnesium-silicon-potassium bioactive fertilizer is applied, and the soil is plowed once. The product is mixed evenly by stirring, and the mixture is applied as fertilizer in the field by digging a ditch and plowing once. The fertilizer has long-lasting effects, and the stack, soil conditioner, and soil modifier can improve soil fertility for a long time. The organic carbon and bio-fermented organic carbon filtrate can provide carbon nutrition for microorganisms in the soil, increase microbial diversity in the soil, and activate calcium-magnesium-silicon-potassium bioactive fertilizer to improve the utilization rate of trace elements by crops, and the effectiveness of chelated trace elements is improved, making them more easily absorbed by crops.

[0025] CN107935783A provides a trace element type liquid water-soluble fertilizer containing amino acids, which includes the following components: cottonseed meal, corn cob, wheat bran, edible mushroom dregs, silkworm chrysalis, periostracum cicadae, discarded animal fur, ferrous sulfate heptahydrate, copper sulfate pentahydrate, zinc sulfate heptahydrate, manganese sulfate monohydrate, sodium borate decahydrate, ammonium molybdate tetrahydrate, complex bacterial agents A and B, earthworm manure, citric acid, pH adjuster, and water. The production process uses modern biotechnology to inoculate complex bacterial agents A and B, and adopts a two-stage aerobic fermentation production process. The agricultural waste, plant proteins contained in edible mushroom dregs, and animal proteins contained in discarded animal fur are decomposed into small-molecule free amino acids, natural active substances, and nutrient ingredients, providing comprehensive protection for the healthy growth of plants. The trace element type liquid water-soluble fertilizer containing amino acids is prepared by chelating with trace element components. The invention makes full use of agricultural waste, edible mushroom dregs, and discarded animal fur, achieving resource reuse and environmental protection without pollution.

[0026] CN117049921A relates to the technical field of water-soluble fertilizer, and specifically discloses a preparation method of earthworm organic water-soluble fertilizer, which comprises the following steps: step one: pretreatment of earthworms, i.e., washing live earthworm raw materials with water to remove impurities; step two: grinding and adding 5 times the mass of water to the washed earthworms; step three: adding the earthworm slurry to a fermentation tank, adding 0.1% of the mass of the earthworms of Bacillus natto, stirring uniformly, heating to 20-40°C, and incubating for 36-48 hours. The earthworm organic water-soluble fertilizer has high organic matter and nutrient content, and the effect is obvious during popularization and application. The addition of montmorillonite to adjust the wollastonite agent optimizes and improves the slurry, and the montmorillonite and wollastonite are optimized and improved together, and the hydroxyapatite blending agent is used, so that the raw materials are mutually coordinated and synergistic, and the fertilizer efficiency and crop quality are effectively improved.

[0027] CN115623894A belongs to the technical field of soil remediation, and discloses a soil improvement method for reducing the absorption of heavy metal cadmium by plants, which comprises adding earthworm manure and alkali-modified biochar to the soil and stirring uniformly; after the plants are planted for 7-10 days, spraying dilute 600 times of alkaline water-soluble fertilizer on the soil, and applying it every 7 days. The present application solves the problem of excessive heavy metal cadmium content in plants due to the absorption of heavy metal cadmium in the soil during planting in the prior art.

[0028] CN118619749A provides a powder organic-inorganic water-soluble fertilizer and a preparation method thereof, and relates to the technical field of water-soluble fertilizer preparation. The powder organic-inorganic water-soluble fertilizer comprises the following raw materials in mass percentage: base fertilizer 10-20%, organic fertilizer 25-40%, inorganic fertilizer 25-45%, and additive 2-4%. The base fertilizer is composed of the following raw materials in weight parts: 10-20 parts of attapulgite, 8-15 parts of seaweed soil, 10-30 parts of earthworm soil, and 12-24 parts of wheat bran. In the traditional water-soluble fertilizer, the base fertilizer is added in the form of powder, which is mixed with water and then enters the soil, ensuring that the plants can obtain sufficient nutrients and that the soil has good fertility, so that the plants can have a long-term good growth, and the number of work operations is reduced.

[0029] CN118666607A relates to a high-amino-acid-content water-soluble fertilizer and its preparation method and application. The Candida lipolytica, Bacillus coagulans, Candida utilis, Bacillus subtilis, Bacillus licheniformis and Lactobacillus plantarum are mixed to obtain a mixed strain, then mixed with brown sugar and sterile water, and cultured to obtain a fermentation inoculum; the fermentation inoculum is diluted and inoculated into fresh pig manure to obtain a pig manure complete fermentation product; the pig manure complete fermentation product is used for earthworm breeding to obtain high-protein earthworms; soybean meal powder is mixed with high-protein earthworms, brown sugar, sterile water and mixed strains to obtain soybean meal bacteria; the soybean meal bacteria are mixed with brown sugar water and mixed strains to obtain soybean meal bacteria fermentation products; the soybean meal bacteria fermentation products are filtered to obtain soybean meal bacteria fermentation liquor; the soybean meal bacteria fermentation liquor is mixed with monoammonium phosphate and potassium carbonate to obtain a high-amino-acid-content water-soluble fertilizer. The high-amino-acid-content water-soluble fertilizer performs well in promoting plant growth and improving plant resistance.

[0030] CN118271137A belongs to the technical field of organic fertilizer, and discloses a liquid organic water-soluble fertilizer, which is uniformly dissolved and mixed with nitrogen-containing organic materials and potassium-containing materials in earthworm manure extract liquid containing microbial flora. The liquid organic water-soluble fertilizer takes the earthworm manure extract liquid containing microbial flora as a matrix mother liquor, and is mixed with various materials including nitrogen-containing organic materials and potassium-containing materials, so that the microbial flora, rich enzyme series and trace elements in the earthworm manure extract liquid are organically combined with nitrogen-containing organic materials and potassium and other macroelements, the structure of soil flora and crop flora can be effectively regulated and improved, various nutrient elements can be better absorbed and converted by crops, growth and development can be promoted, and the frequency of occurrence of diseases and pests can be reduced, so that the quality and yield of crops are improved.

[0031] CN204602025U belongs to the technical field of earthworms, and particularly relates to an earthworm amino acid water-soluble fertilizer preparation tank, which comprises a stirring device, an earthworm amino acid feeding device, a fixing seat, a water-soluble fertilizer preparation solution, a water-soluble fertilizer preparation tank, a stirring shaft, a base, a rotating shaft of a rotating disc, a moving rotating disc, the stirring device, a transverse mechanical linkage mechanism, a motor rotating shaft, a motor, and a belt. The water-soluble fertilizer preparation tank is internally provided with the water-soluble fertilizer preparation solution. The moving rotating disc is provided with the rotating shaft of the rotating disc at the center and a moving hole outside the center. The motor rotating shaft is connected with the rotating shaft of the rotating disc through the belt. The moving hole is connected with the transverse mechanical linkage mechanism through a rotating bearing. The other end of the transverse mechanical linkage mechanism is connected with the stirring shaft. The stirring shaft is located in the water-soluble fertilizer preparation tank. The patent adopts a simple mechanical linkage structure to realize the stirring work of the stirring shaft and greatly reduces the manufacturing cost of the stirrer.

[0032] CN119822890A discloses an amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate, which is obtained by encapsulating earthworm enzymatic stock solution, nitrogen, phosphorus and potassium organic material in microgel; the microgel is obtained by crosslinking chitosan quaternary ammonium salt and cellulose with a crosslinking agent; the positively charged quaternary ammonium salt structure in the microgel plays a role in dispersion, stabilization and antibacterial effect; the slow-release effect of the encapsulated organic water-soluble fertilizer prevents loss of the fertilizer; the obtained microgel has a particle size of 1-20 μm and is convenient to spray.

[0033] CN118271138A belongs to the technical field of ecological fertilizer, and discloses a humic acid-containing water-soluble fertilizer based on earthworm manure extract, which is prepared by adding nutritional additives, humic acid additives and soil conditioners into earthworm manure extract and stirring uniformly, wherein the nutritional additives include nitrogen-containing additives, phosphorus-containing additives and potassium-containing additives. The earthworm manure extract is used as a base, and is combined with nutritional additives and soil conditioners, so that the structure of soil microbial community can be improved, the pH value of soil can be adjusted, the soil can be supplemented with macroelements and microelements, nutrient loss can be reduced, the fertilizer utilization rate can be improved, the frequency of crop diseases and insect pests can be reduced, the growth and development of crops can be promoted, and the quality and yield of crops can be further improved.

[0034] CN110800760B provides an earthworm hydrolysate and a preparation method and application thereof, and belongs to the technical field of biological fertilizer. The earthworm hydrolysate comprises the following raw materials: earthworms and urea; and the mass ratio of the earthworms to the urea is 3-7:1. In the present application, urea as a denaturing agent can denature and inactivate the proteins (such as outer membrane proteins) non-covalently bound on the cytoplasmic membrane, and increase the solubility of these proteins. When the urea is mixed with the earthworms, the cytoplasmic membrane structure of the earthworms is destroyed, the intracellular substances rapidly leak out, and the protein hydrolase effectively hydrolyzes the earthworm body proteins without the need for live earthworms and the provision of suitable living conditions for the live earthworms. The obtained hydrolysate has a near-neutral pH value and high antibacterial activity. Meanwhile, the urea used in the present application is a chemical fertilizer commonly used in agricultural production, which is mixed with earthworms to form organic-inorganic compound fertilizer after hydrolysis, and can be directly used for the preparation of water-soluble fertilizer to provide more abundant nutrients for plants.

[0035] CN104872189A belongs to the technical field of preservatives, and particularly relates to a novel bioactive preservative, characterized in that the bioactive preservative comprises EM bacteria and RW bacteria. The preservative has a shelf life of 1-2 years, is safe and efficient, has no drug residue, and has no toxic side effects. The earthworm extract prepared by the method contains more than 300 million CFU of beneficial bacteria per gram. The water-soluble fertilizer prepared by using the product can improve the nutrient absorption and utilization rate of crops, and increase the yield of crops by 10-25%.

[0036] On February 25, 2025, an abstract search was conducted on China National Knowledge Infrastructure with "Earthworm and water and soluble and fertilizer and ammonium and sulfate" and no relevant literature was found.

[0037] On February 25, 2025, a search was conducted on the United States Patent and Trademark Office website with "Earthworm and water and soluble and fertilizer and ammonium and sulfate" and no relevant literature was found; search website https: / / ppubs.uspto.gov / pubwebapp / .

[0038] On February 25, 2025, a search was conducted on WIPO's https: / / patentscope2.wipo.int / with "Earthworm and water and soluble and fertilizer and ammonium and sulfate" and no relevant literature was found.

[0039] On February 25, 2025, a search was conducted on the Japanese Patent Office's website https: / / www.j-platpat.inpit.go.jp / with "Earthworm and water and soluble and fertilizer and ammonium and sulfate" and no relevant literature was found.

[0040] And the idea of this patent is completely different.

[0041] The above scheme has typical defects in that: First: all earthworm-related fertilizers must be compounded with some elements, so the cost is high; Second: all earthworm-related fertilizers cannot be combined with the ingenious earthworm-providing process, and the whole body of earthworms is not utilized.

[0042] Third: ammonium sulfate is a main raw material used as a fertilizer, which needs to be purchased separately.

[0043] Fourth: there is no application process for the whole earthworm industry chain.

[0044] Fifth: in the preparation of various fertilizers, the treasure use of earthworms is not fully utilized.

[0045] Sixth: a large amount of organic solvent is used and can only be wasted as waste liquid. SUMMARY

[0046] The purpose of the application is to provide earthworm amino acid water-soluble fertilizer with better effect and use method, and the specific purposes are the multiple substantial technical effects in the specific implementation part.

[0047] In order to achieve the above purposes, the application adopts the following technical solutions: The earthworm amino acid water-soluble fertilizer, The preparation of the crude extract includes the following steps: taking earthworm powder as raw material, adding buffer solution with a mass ratio of 1:5, the buffer solution is composed of 10 mmol / L Tris-HCl and 145 mmol / L NaCl, and the pH is 7.4, after fully stirring and dissolving, it is stored at 4 DEG C overnight, centrifuged at 10,000 r / min for 15 min, and the supernatant is taken; the centrifugal residue is continuously added with a certain amount of buffer solution for stirring and secondary extraction for 2 h, and then the supernatant is taken again, the two supernatants are combined and filtered with a defatted cotton column for standby; The ammonium sulfate-membrane separation extraction method: the crude extract is slowly added with solid ammonium sulfate, fully stirred and dissolved, the ammonium sulfate saturation is adjusted to 80%, after being stored at 4 DEG C overnight, the supernatant and earthworm kinase precipitate are separated by centrifugation at 10,000 r / min for 15 min; the precipitate is dissolved with a certain amount of 50 mmol / L Tris-HCl solution, and then purified and desalted by using an ultrafiltration membrane to obtain earthworm kinase liquid; after measuring the specific activity of the enzyme, the total activity recovery rate and the purification multiple are calculated; The application is characterized in that, In the extraction process of the earthworm kinase of Eisenia foetida, the residue filtered in the preparation process of the crude extract is used as raw material one, and the raw material one is the remaining material except the supernatant; The raw material two is the material after membrane filtration in the ammonium sulfate-membrane separation extraction method; The two are mixed in a volume ratio of raw material one: raw material two = 3:1, and then there are both inorganic nitrogen sources and organic amino acid precursors, which meet the basic conditions of water-soluble fertilizer.

[0048] The further technical solution of the application is that, The waste liquid is combined according to the ratio of raw material one: raw material two, and mixed uniformly; Protease is added for further hydrolysis, 45 DEG C, 4 h; Firstly, 200 kD coarse filtration is carried out, then 10 kD fine filtration is carried out, and then 1 kD membrane separation is carried out to remove impurities, so that free amino acids and small molecule peptides with a size less than 1 kD are obtained; Reverse osmosis membrane pre-concentration is carried out, and the solid content is increased to 40%, which meets the requirements of the national water-soluble fertilizer industry standard.

[0049] The finished product is adjusted to pH 5.5-6.5 by citric acid / potassium hydroxide.

[0050] The further technical solution of the application is that the protease is trypsin.

[0051] The further technical solution of the present application is further characterized in that the chitosan is 1-2% of the mass of the water-soluble fertilizer product, and the surface gelation can be completed within 1-5 minutes after spraying, so as to form a basic barrier layer. The chitosan here is pure chitosan or a chitosan compound system.

[0052] The further technical solution of the present application is further characterized in that the film formation refers to the formation of a facial mask on the surface of the plant, and the facial mask is quickly formed; the chitosan has a molecular weight of less than or equal to 33 kDa; the chitosan compound system is 1% chitosan+2% fulvic acid+compound 4% pre-emulsified beeswax to form a hydrophobic layer, which needs to be double-filtered before atomization, first 100 μm coarse filtration, and then 5 μm fine filtration to prevent blockage, and the film formation is completed within 1-3 minutes, and the complete solidification is completed within 10-15 minutes.

[0053] The further technical solution of the present application is further characterized in that the film formation refers to the formation of a ground slow-release film on the surface of the soil, and the ground slow-release film has the functions of decomposition and antibacterial; the chitosan has a molecular weight of 100-200 kDa; the chitosan compound system is 5% chitosan+1% glycerol+0.05% chitooligosaccharide of the mass of the water-soluble fertilizer product, and the chitooligosaccharide has a molecular weight of 1 kDa+0.5% urea; the chitooligosaccharide and the urea activate the original actinomycetes in the soil as a carbon source; the traditional high-pressure spraying is adopted, the film formation is completed within 5-8 minutes, the thickness is uneven (5-15 μm), the degradation is started within 5-7 days, and the antibacterial and degradation functions are simultaneously realized.

[0054] The further technical solution of the present application is further characterized in that the chitosan compound system and the water-soluble fertilizer product are respectively diluted, then mixed through pressure transmission at a three-way joint into a spray pipe, and then sprayed from a spray head.

[0055] The further technical solution of the present application is further characterized in that the chitosan compound system and the water-soluble fertilizer product are stored in different containers and compounded before fertilization and pesticide application.

[0056] The use method of the earthworm amino acid water-soluble fertilizer is characterized in that the chitosan compound system and the water-soluble fertilizer product are stored in different containers, respectively diluted with water, and then sprayed through pressure transmission after mixing through a three-way joint.

[0057] The further technical solution of the present application is further characterized in that the chitosan compound system and the water-soluble fertilizer product are mixed and sprayed on plants or the ground where the plants grow.

[0058] The present application adopting the above technical solution has the following beneficial effects compared with the prior art: in view of the defect "first, all earthworm-related fertilizers must be compounded with some elements, so the cost is high", the present application creatively utilizes the comprehensive utilization of waste liquid to realize the preparation of water-soluble fertilizer without the need of compounding some elements.

[0059] For the defect "second: all the earthworm related fertilizer can not and earthworm provide process clever combination, not to the whole body of earthworm make use of." This patent groundbreaking use of water-soluble fertilizer and earthworm kinase comprehensive production, provides a new industrial chain, the industrial chain can be used for earthworm all, and will not produce waste.

[0060] For the defect "third: ammonium sulfate is a kind of main raw material for fertilizer, all need to buy separately." This patent groundbreaking use of ammonium sulfate as earthworm kinase extract material and as a fertilizer clever combination, let the process no emission.

[0061] For the defect "fourth: there is no any earthworm whole industry chain application process." This patent groundbreaking provides the whole industry chain of earthworm.

[0062] For the defect "fifth: in the preparation of various fertilizers, the treasure of earthworm is not fully classified and utilized." This patent groundbreaking use of technology, after the classification of earthworm kinase, earthworm kinase is used as high-end product, the rest of the ingredients are used, and the extract material ammonium sulfate is comprehensive water-soluble fertilizer.

[0063] For the defect "sixth: a large number of organic solvents are used and can only be wasted as waste liquid." This patent groundbreaking use of process combination, no waste is produced. And, no any pollution of organic solvents. BRIEF DESCRIPTION OF DRAWINGS

[0064] In order to further illustrate the present application, further illustrated below in conjunction with the drawings: Figure 1 For the flow chart of the invention; Figure 2 For the special pipe of the invention double substance injection. DETAILED DESCRIPTION

[0065] The present invention will be further explained below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the following specific embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention. In the description of the present invention, it should be noted that the orientation or position relationship indicated by the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", "top", "bottom", etc. is based on the orientation or position relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation of the present invention. In addition, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, or it can be a communication between the internal parts of two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0066] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0067] This patent provides multiple parallel solutions. The differences in descriptions are improvements or parallel solutions based on the basic solution. Each solution has its own unique characteristics. In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other. Fixing methods not described in this article can be any fixing method such as threaded fixing, bolt fixing, or gluing.

[0068] Example 1: Combination Figure 1 ; Earthworm-derived amino acid water-soluble fertilizer, The preparation of the crude extract includes the following steps: weighing earthworm fine powder raw material, adding buffer solution at a mass ratio of 1:5, the buffer solution composition is 10 mmol / L Tris-HCl, 145 mmol / L NaCl, pH 7.4, after fully stirring and dissolving, storing overnight at 4°C, centrifuging at 10,000 r / min for 15 min, and taking the supernatant; the centrifugal residue is continuously added with a certain amount of buffer solution for stirring and secondary extraction for 2 h, and then the supernatant is taken again after centrifugation, and the two supernatants are combined and filtered with a defatted cotton column for standby; Ammonium sulfate-membrane separation extraction method: slowly add solid ammonium sulfate to the crude extract, fully stir and dissolve, adjust the ammonium sulfate saturation to 80%, store overnight at 4°C, then centrifuge at 10,000 r / min for 15 min to separate the supernatant and earthworm kinase precipitate; after dissolving the precipitate in a certain amount of 50 mmol / L Tris-HCl solution, purify and desalt using an ultrafiltration membrane to obtain an earthworm kinase solution; after measuring the specific activity of the enzyme, calculate the total activity recovery rate and purification fold; It is characterized in that, In the extraction process of Eisenia foetida kinase, the residue filtered in the preparation process of the crude extract is used as raw material I, which is the remaining material except the supernatant; Raw material II is the material after membrane filtration in the ammonium sulfate-membrane separation extraction method; Mix the two at a volume ratio of raw material I: raw material II = 3:1, which provides both inorganic nitrogen source and organic amino acid precursor, meeting the basic conditions of water-soluble fertilizer.

[0069] Combine the waste liquid according to the ratio of raw material I: raw material II, and mix well; Add protease for further hydrolysis at 45°C for 4 h; Firstly, 200kD coarse filtration, then 10kD fine filtration, and finally 1kD membrane separation to remove impurities, so as to obtain free amino acids and small molecule peptides with a molecular weight less than 1kD; Pre-concentrate with reverse osmosis membrane, and increase the solid content to 40%, which meets the requirements of the national water-soluble fertilizer industry standard.

[0070] Adjust the pH of the finished product to 5.5-6.5 with citric acid / potassium hydroxide.

[0071] The above scheme is closely matched with the "Extraction process of Eisenia foetida kinase and enzymatic properties", and the waste is utilized completely.

[0072] In view of the defect "First of all, all earthworm-related fertilizers must be compounded with some elements, so the cost is high;", this patent creatively utilizes the comprehensive utilization of waste liquid, which can realize the preparation of water-soluble fertilizer without compounding some elements.

[0073] For the defect "second: all earthworm-related fertilizers cannot be combined with the earthworm-provided process, and the whole earthworm is not utilized." This patent creatively utilizes the comprehensive production of water-soluble fertilizer and earthworm kinase to provide a brand-new industrial chain, which can utilize the whole earthworm and will not produce waste.

[0074] For the defect "third: ammonium sulfate is a main raw material for fertilizer, which needs to be purchased separately." This patent creatively utilizes the ingenious combination of ammonium sulfate as an earthworm kinase extraction material and as a fertilizer, making the process emission-free.

[0075] For the defect "fourth: there is no application process for the whole earthworm industry chain." This patent creatively provides a whole earthworm industry chain.

[0076] For the defect "fifth: in various fertilizer preparations, the valuable use of earthworms is not fully utilized." This patent creatively utilizes technology to extract earthworm kinase, uses earthworm kinase as a high-end product, and uses the remaining ingredients as water-soluble fertilizer with ammonium sulfate.

[0077] For the defect "sixth: a large amount of organic solvent is used and can only be wasted as waste liquid." This patent creatively utilizes the process combination, without any waste generation. Moreover, no polluting organic solvent is used.

[0078] Example two: as a further improved scheme or parallel scheme or alternative independent scheme, the protease is trypsin. This patent non-obviously creatively further decomposes earthworm protein, and as a preferred, the protease is alkaline protease, acid protease, or complex protease.

[0079] Example three: as a further improved scheme or parallel scheme or alternative independent scheme, it also contains auxiliary chitosan; chitosan is 1-2% of the mass of water-soluble fertilizer product, and surface gelation can be completed within 1-5 minutes after spraying, forming a basic barrier layer. The chitosan here is pure chitosan or a chitosan complex system. This patent non-obviously creatively makes water-soluble fertilizer into foliar fertilizer or mulch fertilizer, etc.

[0080] Example four: as a further improved scheme or parallel scheme or alternative independent scheme, film formation refers to the formation of a facial mask on the surface of the plant, and the facial mask forms a film quickly; the molecular weight of chitosan is ≤33 kDa, and the chitosan complex system is 1% chitosan + 2% fulvic acid + complex 4% pre-emulsified beeswax to form a hydrophobic layer, which needs to be double-filtered before atomization, first 100 μm coarse filter, then 5 μm fine filter to prevent blockage, 1-3 minutes film formation, and 10-15 minutes complete curing.

[0081] The patent non-obviously initiatively provides foliar fertilizer.

[0082] Example five: As a further improvable scheme or parallel scheme or alternative independent scheme, film formation refers to the formation of a ground slow-release film on the soil surface, which has the functions of decomposition and bacteriostasis. The chitosan molecular weight is 100-200 kDa, the chitosan compound system is 5% chitosan+1% glycerol+0.05% chitosoligosaccharide of the mass of water-soluble fertilizer product, the chitosan oligosaccharide molecular weight is 1 kDa+0.5% urea, and the chitosan oligosaccharide and urea activate the soil original actinomycetes as a carbon source. The traditional high-pressure spraying is adopted, the film is formed in 5-8 minutes, the thickness is uneven (5-15 μm), the degradation is started in 5-7 days, and the bacteriostasis and degradation are realized synchronously. The patent non-obviously initiatively provides ground fertilizer.

[0083] Example six: As a further improvable scheme or parallel scheme or alternative independent scheme, the chitosan compound system and the water-soluble fertilizer product are respectively diluted, then mixed into the spray pipe through pressure delivery at the three-way joint, and then sprayed from the spray head. The patent non-obviously initiatively provides a combined spraying structure for Figure 2 the spraying of example five and example six. Avoiding early mixing will block the spray head.

[0084] Example seven: As a further improvable scheme or parallel scheme or alternative independent scheme, the chitosan compound system and the water-soluble fertilizer product are stored in different containers and compounded before fertilization and spraying.

[0085] Example eight: As a further improvable scheme or parallel scheme or alternative independent scheme, the use method of earthworm-derived amino acid water-soluble fertilizer is characterized in that the chitosan compound system and the water-soluble fertilizer product are stored in different containers, respectively diluted with water, and then sprayed through pressure delivery after mixing through the three-way joint.

[0086] Example nine: As a further improvable scheme or parallel scheme or alternative independent scheme, the chitosan compound system and the water-soluble fertilizer product are mixed and sprayed on plants or the ground where plants grow. For the ground, it can also be kept moist.

[0087] As a discussion: 1. Ammonium sulfate (nitrogen content 21%) but the disadvantage is high concentration of ammonium ion, direct application may burn seedlings 2. Earthworm leachate in leachate contains various amino acids.

[0088] 3. Inorganic salt: NaCl, Tris-HCl in buffer can provide electrolyte and promote plant absorption.

[0089] Combined with 0.5% glycerol + 2% fulvic acid (fulvic acid promotes condensation, glycerol plasticizing prevents cracking), it can be formed in about 2 minutes.

[0090] After atomization, it can be ultra-thin, with a thickness of about 2-5 μm. Atomization and film formation are better, but the cost is also high. In the open field, replace beeswax with 0.1% Tween 80 to reduce cost.

[0091] Pioneeringly, the above effects exist independently, and a set of structures can also achieve the combination of the above results.

[0092] It should be noted that the multiple schemes provided by the present patent contain the basic scheme itself, are independent of each other, and do not restrict each other, but they can also be combined with each other without conflict to achieve multiple effects together.

[0093] The above shows and describes the basic principles, main features and advantages of the present application. Those skilled in the art should understand that the present application is not limited to the above examples, and the above examples and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of protection.

Claims

1. Earthworm-derived amino acid water-soluble fertilizer, The method comprises the following steps: weighing earthworm powder raw material, adding a buffer solution at a mass ratio of 1:5, wherein the buffer solution comprises 10 mmol / L Tris-HCl and 145 mmol / L NaCl, and has a pH of 7.4; stirring thoroughly to dissolve the solution; refrigerating the solution at 4°C overnight; centrifuging the solution at 10,000 rpm for 15 minutes; and collecting the supernatant; adding a certain amount of buffer solution to the centrifugal residue and stirring the solution for a second extraction for 2 hours; centrifuging the solution again to collect the supernatant; combining the two supernatants and filtering the solution through a cotton ball column for later use; Ammonium sulfate-membrane separation extraction method: Solid ammonium sulfate was slowly added to the crude extract, thoroughly stirred and dissolved, and the ammonium sulfate saturation was adjusted to 80%. After refrigeration at 4°C overnight, the supernatant and lumbrokinase precipitate were centrifuged at 10,000 rpm for 15 minutes to separate. The precipitate was dissolved in a certain amount of 50 mmol / L Tris-HCl solution, purified and desalted using an ultrafiltration membrane to obtain the lumbrokinase solution. The enzyme specific activity was measured and the total activity recovery and purification factor were calculated. It is characterized in that In the extraction process of lumbrokinase using Eisenia fetida, the residue filtered during the preparation of the crude extract is used as raw material one, which is the remaining material except the supernatant; Raw material 2 is the material after membrane filtration in ammonium sulfate-membrane separation and extraction method; Raw material 1: Raw material 2 = 3:1 volume ratio. Mix the two, and you will have both inorganic nitrogen source and organic amino acid precursor, which meets the basic conditions of water-soluble fertilizer.

2. The earthworm-derived amino acid water-soluble fertilizer according to claim 1, wherein Combine the waste liquid in the ratio of raw material 1: raw material 2 and mix well; Protease was added for further hydrolysis at 45°C for 4 h; First, 200kD coarse filtration, then 10kD fine filtration, and then 1kD membrane separation to remove impurities, the goal is to obtain free amino acids and small molecule peptides less than 1kD; Reverse osmosis membrane pre-concentration increases the solid content to 40%, meeting the requirements of the national water-soluble fertilizer industry standards; The finished product is adjusted to pH 5.5-6.5 with citric acid / potassium hydroxide.

3. The earthworm-derived amino acid water-soluble fertilizer according to claim 2, wherein The protease is trypsin.

4. The earthworm-derived amino acid water-soluble fertilizer according to claim 1, wherein The finished product also contains auxiliary chitosan when it is released; chitosan accounts for 1-2% of the mass of the finished water-soluble fertilizer. It can complete surface gelation 1-5 minutes after spraying to form a basic barrier layer. The chitosan here is pure chitosan or a chitosan compound system.

5. The earthworm-derived amino acid water-soluble fertilizer according to claim 4, characterized in that: Film formation refers to the formation of a mask on the surface of the plant, and the mask forms quickly; the molecular weight of chitosan is ≤33 kDa, and the chitosan compound system is 1% chitosan + 2% fulvic acid + a compound of 4% pre-emulsified beeswax of the mass of the finished water-soluble fertilizer to form a hydrophobic layer. Double-stage filtration is required before atomization, first 100 μm coarse filtration, then 5 μm fine filtration to prevent clogging, film formation in 1-3 minutes, and complete solidification in 10-15 minutes.

6. The earthworm-derived amino acid water-soluble fertilizer according to claim 4, characterized in that: Film formation refers to the formation of a ground slow-release film on the soil surface. The ground slow-release film takes into account both decomposition and antibacterial properties. The molecular weight of chitosan is 100-200 kDa. The chitosan compound system is 5% chitosan + 1% glycerol + 0.05% chitosan oligosaccharide of the mass of the finished water-soluble fertilizer. The molecular weight of chitosan oligosaccharide is 1kDa + 0.5% urea. Chitosan oligosaccharide and urea are used as carbon sources to activate native actinomycetes in the soil. Traditional high-pressure spraying is used. The film is formed in 5-8 minutes with uneven thickness (5-15 μm). Degradation starts in 5-7 days, and antibacterial and degradation are achieved simultaneously.

7. The earthworm-derived amino acid water-soluble fertilizer according to any one of claims 4 to 6, characterized in that: The chitosan compound system and the water-soluble fertilizer product are diluted separately, then mixed into the nozzle at the tee by pressure delivery, and then sprayed out from the nozzle.

8. The earthworm-derived amino acid water-soluble fertilizer according to any one of claims 7, wherein: The chitosan compound system and the finished water-soluble fertilizer are stored in different containers and compounded before fertilizing and spraying.

9. The method for using the earthworm-derived amino acid water-soluble fertilizer is characterized in that: Using the earthworm-derived amino acid water-soluble fertilizer according to any one of claims 1 to 8, the chitosan compound system and the water-soluble fertilizer product are stored in different containers, each is diluted with water, and then mixed through a three-way connection by pressure feeding and then sprayed out.

10. The method for using the earthworm-derived amino acid water-soluble fertilizer according to claim 9, wherein: The chitosan compound system and the finished water-soluble fertilizer are mixed and then sprayed on the plants or the ground where the plants grow.

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