Large-scale cultivation method and quality detection method of cordyceps militaris fruit bodies

A cultivation method and grass seed technology are applied in the field of large-scale cultivation and quality inspection of fruiting bodies of Cordyceps militaris, and can solve problems such as uneven quality, affecting the healthy development of Cordyceps militaris application and Cordyceps militaris industry, and large differences in active ingredient content.

Inactive Publication Date: 2012-08-08
珠海市先康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quality of Cordyceps militaris sporocarps on the market varies greatly. According to the test results provided by relevant testing agencies, the content of active ingredients in different manufacture

Method used

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  • Large-scale cultivation method and quality detection method of cordyceps militaris fruit bodies
  • Large-scale cultivation method and quality detection method of cordyceps militaris fruit bodies
  • Large-scale cultivation method and quality detection method of cordyceps militaris fruit bodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1. Preparation of solid medium: rice is used as the main raw material of solid medium, and the proportions of each component per 100 grams of medium are: 50 parts of rice, 50 parts of nutrient solution, and then sterilized at a pressure of 0.12MPa and a temperature of 121°C 20min. The ingredients of the original nutrient solution are: glucose 5g / L, soybean 8g / L, milk powder 5g / L, triammonium citrate 1.0 g / L, potassium dihydrogen phosphate 1.0 g / L, magnesium sulfate 1.0 g / L, vitamin B 1 10mg / L.

[0063] 2. Preparation of liquid strains: inoculate the original strains on PDA (potato dextrose agar medium) medium, culture in dark at 23°C for 7 days to obtain activated strains. Put the activated strains into the liquid culture medium, culture them statically for 24 hours, place them on a shaker for suspension culture, and culture them in the dark at 23°C for 6 days, crush the liquid strains with a magnetic stirrer and then use them for inoculation .

[0064] 3. Fruiting b...

Embodiment 2

[0066] 1. Preparation of solid medium: the solid medium is rice solid medium, and each component in the mixed medium per hundred parts by weight is: 50 parts of rice, 50 parts of nutrient solution, and then at a pressure of 0.12MPa and a temperature of 121°C Sterilize for 20 minutes. The ingredients of the original nutrient solution are: glucose 5g / L, soybean 8g / L, milk powder 5g / L, triammonium citrate 1.0 g / L, potassium dihydrogen phosphate 1.0 g / L, magnesium sulfate 1.0 g / L, vitamin B 1 10mg / L, the pH value is 5.5~6.0.

[0067] All the other operating steps are the same as in Example 1.

Embodiment 3

[0069] 1. Preparation of solid medium: the solid medium is rice solid medium, and each component in the mixed medium per hundred parts by weight is: 50 parts of rice, 50 parts of nutrient solution, and then at a pressure of 0.12MPa and a temperature of 121°C Sterilize for 20 minutes. The ingredients of the original nutrient solution are: sucrose 5g / L, soybean 8g / L, milk powder 5g / L, triammonium citrate 1.0 g / L, potassium dihydrogen phosphate 1.0 g / L, magnesium sulfate 1.0 g / L, vitamin B 1 10mg / L, the pH value is 5.5~6.0.

[0070] All the other operating steps are the same as in Example 1.

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Abstract

The invention discloses a large-scale cultivation method and a quality detection method of cordyceps militaris fruit bodies, comprising the steps of: (1) screening spawn; (2) preparing a rice solid culture medium (40-55 parts of rice and 45-60 parts of nutrient solution in every 100 parts by weight) and killing bacteria; (3) preparing liquid spawn: activating the spawn, inoculating the spawn into a liquid culture medium, conducting static culture for 24 hours and performing suspension culture on a shaking table; (4) culturing fruit bodies: inoculating the liquid spawn on the solid culture medium for dark culture for 6 days at the temperature of 20 DEG C until the surface of the medium is covered by mycelium; placing the well grown mycelium under incandescence with light intensity of 100 to 300 lux, wherein during a primordium growth phase, illumination is performed for 20-24 hours per day at the temperature of 20-24 DEG C; and during a fruit bodies growth phase, illumination is performed for 8-12 hours per day at the temperature of 18-20 DEG C and the fruit bodies are harvested 5 to 8 days after mature. The cordyceps militaris fruit bodies cultured by the method have the advantages of high yield and high content of active ingredients. Batches of cordyceps militaris fruit bodies have fingerprints with a similarity value greater than 0.950, indicating stable and controllable quality.

Description

[0001] technical field [0002] The invention relates to a large-scale cultivation method and a quality detection method of the fruit body of Cordyceps militaris. Background technique [0003] The Cordyceps fungus belongs to the genus Ergotaceae Cordyceps of the Ascomyceta Hypocreaes, and there are more than 350 species in the world, and there are nearly 60 species in my country. Cordyceps sinensis and Cordyceps militaris are the two most researched among the numerous insect-borne fungi. Cordyceps sinensis Cordyceps sinensis (Berk.) Sace. Parasitic on bat moths of the bat moth family insects Hepialus armoranus Oberthür The complex of the sub-seat and the insect body on the overwintering larvae is a national second-class key protected plant. It is mainly distributed in alpine meadows between 4200 and 5400 meters above sea level. Although Cordyceps sinensis plays an important role in traditional Chinese medicine, as a model species of the genus Cordyceps, Cordyceps militar...

Claims

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Application Information

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IPC IPC(8): A01G1/04G01N30/02
Inventor 吴美兰吴更余李秋洋黄杏云钟美婵郑令文姚磊
Owner 珠海市先康生物科技有限公司
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