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Fermentation method for accelerating ganoderic acids and ganoderma lucidum polysaccharide biological synthesis

A fermentation method and technology of Ganoderma lucidum polysaccharides, applied in the field of microbial fermentation, can solve the problems such as the output or content of Ganoderma lucidum acid and Ganoderma lucidum polysaccharides needs to be improved

Inactive Publication Date: 2008-08-06
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above-mentioned documents, no corresponding combined control strategy was adopted to promote the growth of lucidum cells and the synthesis of products according to the inconsistency of the environmental conditions (such as pH and dissolved oxygen concentration) required for the growth of ganoderma lucidum cells and the synthesis of products, so its ganoderma acid and Ganoderma lucidum polysaccharide production or content needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The purpose of this experiment is to investigate the effects of different pH values ​​in the liquid fermentation system on the growth of Ganoderma lucidum cells and the biosynthesis of Ganoderma acid or / and Ganoderma polysaccharides.

[0030] 1. The cultivation of ganoderma lucidum slant strains, primary liquid seed cultivation, and secondary liquid seed cultivation are carried out according to the following medium and cultivation conditions:

[0031] Described slant culture medium is PDA agar medium (magnesium sulfate heptahydrate of 1.5 grams / liter, potassium dihydrogen phosphate of 3.0 grams / liter, glucose of 20 grams / liter, vitamin B2 of 0.05 grams / liter 1 and 200 g of potatoes / liter of extract). The cultivation conditions of the ganoderma lucidum slant strains are: cultivation temperature 28° C., dark cultivation, and cultivation time 7 days.

[0032] The first-level liquid seed culture medium is: 35 grams / liter of glucose, 5 grams / liter of peptone, 2.5 grams / lite...

Embodiment 2

[0043] The purpose of this experiment is to investigate the effect of liquid fermentation system using two pH stages on the growth of Ganoderma lucidum cells and the biosynthesis of Ganoderma acid or / and Ganoderma polysaccharides.

[0044] In the present embodiment, the cultivation of ganoderma lucidum slant strains, the primary liquid seed cultivation and the secondary liquid seed cultivation are completely the same as in Example 1;

[0045] The medium and culture temperature of the submerged fermentation are the same as in Example 1, and the difference from Example 1 is that 4 groups of parallel experiments are used, and each group of experiments adopts a pH two-stage control strategy to produce ganoderma acid and ganoderma polysaccharides during submerged fermentation :

[0046] The first stage: each group controls the pH value of the fermentation culture system to 3.0, so that the number of Ganoderma lucidum cells can quickly reach a certain range;

[0047] The second sta...

Embodiment 3

[0053] The purpose of this experiment was to investigate the effects of two stages of dissolved oxygen (DO) on the growth of Ganoderma lucidum cells and the biosynthesis of Ganoderma lucidum acid or / and Ganoderma lucidum polysaccharides in the liquid fermentation system.

[0054] Tang et al. (Tang YJ, Zhong JJ. (2003) Role of oxygen supply in submerged fermentation of Ganoderma lucidum for production of Ganoderma polysaccharide and ganoderic acid. Enzyme Microb. Technol. 32: 478-484.) found that 25% dissolved oxygen concentration is beneficial to Ganoderma lucidum Cell growth, dissolved oxygen concentration of 10% is conducive to the biosynthesis of metabolites. However, in practice, it is difficult to predict whether the two-stage control strategy of dissolved oxygen concentration can maximize the growth of Ganoderma lucidum cells and the production of metabolites at the same time. In order to investigate the effect of the two-stage control method of dissolved oxygen concentr...

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Abstract

The invention discloses a fermentation method for promoting mythic fungus acid or mythic fungus polysaccharide biology to synthesize. The invention investigates the condition which is needed in the growth phase of mythic fungus cell and product synthesis phase, which respectively investigates the influence of control strategies of pH two phases and oxygen dissolution two phases to mythic fungus acid or mythic fungus polysaccharide biology according to unconsistency of environmental conditions which are needed in the phases of mythic fungus cell growth and production synthesis, and the invention respectively investigates the influence to mythic fungus acid or mythic fungus polysaccharide biology synthesis after control strategies of pH two phases and oxygen dissolution two phases or the fed strategy are mutually coordinated together on the basis of combining with an intermediate fed strategy. The test result shows that mythic fungus cells are obviously prompted to grow no matter respectively adopting a pH value two phases control strategy, an oxygen dissolution concentration two phases control strategy, or coordinating the pH value two phases control strategy and the oxygen dissolution concentration two phases control strategy with the fed strategy together, and the content or yield of mythic fungus acid or mythic fungus polysaccharide are greatly improved.

Description

technical field [0001] The invention relates to a microbial cell culture method, in particular to a fermentation method for promoting the growth of ganoderma lucidum cells and the biosynthesis of ganoderma polysaccharides and ganoderma acid, belonging to the field of microbial fermentation. Background technique [0002] Ganoderma lucidum (Leyss ex Fr.) Krast.) belongs to Basidiomycetes, Polyporaceae, Ganoderma higher fungi. Traditional Chinese medicine believes that Ganoderma lucidum is sweet in taste, warm in nature, and non-toxic. It can nourish the qi of the heart, liver, spleen, lung, and kidney, and has the effects of nourishing and strengthening the body, strengthening the body and strengthening the body. Modern medical research has found that the active ingredients contained in Ganoderma lucidum are mainly Ganoderma polysaccharides and Ganoderma acid, and Ganoderma acid and Ganoderma polysaccharides have anti-cancer and anti-AIDS effects (Russell R, Paterson M. (2006)...

Claims

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Application Information

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IPC IPC(8): C12P7/40C12P19/04C12R1/645
Inventor 汤亚杰张伟
Owner HUBEI UNIV OF TECH
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