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Cattleya pollen germination medium

A pollen germination and culture medium technology, applied in the direction of plant cells, can solve the problems of cumbersome solid medium preparation, time-consuming, low germination rate, etc., and achieve the effects of short time-consuming, high germination rate and improved work efficiency

Inactive Publication Date: 2012-08-01
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It takes a long time, the germination rate is low, and the preparation of solid medium is cumbersome

Method used

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Effect test

specific Embodiment approach

[0010] Cattleya pollen germination medium of the present invention, its preferred embodiment is:

[0011] The medium is a liquid medium, which contains sucrose, H 3 BO 3 , CaCl 2 . The preferred combination is: the sucrose, H 3 BO 3 , CaCl 2 Concentrations in liquid medium are: 100g L -1 , 40mg L -1 , 150mg L -1 .

[0012] Adopt the Cattleya pollen germination medium of the present invention, just can observe the germination phenomenon of pollen after 3 hours, after 24 hours, pollen piece is dissolved in the nutrient solution completely, can clearly observe the germination of pollen, so use the pollen of the present invention Cattleya pollen germination medium can greatly increase the efficiency. At the same time, it also provides technical support for effectively carrying out research on cattleya hybrid breeding. If appropriate hormones or other active substances are added, the time it takes for cattleya to germinate may be shorter and the germination rate may be hi...

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PUM

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Abstract

The invention discloses Cattleya pollen germination medium, which is liquid medium comprising cane sugar, H3BO3 and CaCl2. Preferably, concentrations of the cane sugar, the H3BO3 and the CaCl2 are respectively 100g / L-1, 40mg / L-1 and 150mg / L-1. By using the Cattleya pollen germination medium, germination of pollen can be observed after 3 hours, pollen mass completely dissolves in the liquid after 24 hours, germination of the pollen can be observed vividly, and efficiency can be improved greatly.

Description

technical field [0001] The invention relates to a pollen germination medium, in particular to a cattleya pollen germination medium. Background technique [0002] Cattelan, family Orchideceae, subfamily Epidendroideae, tribe Epidendreae, subtribe Laeliinae, also known as Cattleya orchid, epiphytic compound stem Orchids. It has colorful flowers, large and colorful flowers, fragrant and pleasant smell, and rich and colorful colors. It has the reputation of "king of orchids" in the world. It is native to tropical and subtropical America, among which Colombia and Brazil are the most wild, and countries such as Colombia, Brazil, and Costa Rica even use Cattleya as their national flower. Cattelan is produced on a large scale in Thailand, Singapore, Indonesia, Malaysia, the Philippines and Taiwan, and these areas have become the main producing areas of Cattelan in the world. [0003] At present, there are about 110 native species of Cattleya (http: / / apps.kew.org / wcsp / home.do), bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 王雁郑宝强
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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