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Vegetative propagation method for butterfly orchid

A technology of asexual reproduction and Phalaenopsis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of many seedling variations, inconsistent seedling growth, less development of lateral buds, etc. Social benefits, the effect of solving source problems

Inactive Publication Date: 2012-08-01
吴海红
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phalaenopsis is a single-stem aerial orchid. The plant itself does not tiller and rarely develops side buds. Due to the particularity of its flower structure, pollination cannot be completed under natural conditions, so it is difficult to obtain seeds; artificial pollination is used to obtain seeds, and the germination of seeds The rate is only about 20%. Inducing its seed germination on the artificial medium has opened up a new rapid propagation route for the development of Phalaenopsis industrialization, but the seedlings that are propagated have many variations and the growth of seedlings is inconsistent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Select the flower stalk of the mature mother plant "Qianhui Rose" of Phalaenopsis that has just taken flower arrows and buds as explants, wash it with washing powder water, and then rinse it under running water for 30-40 minutes, and wash it on the ultra-clean workbench. A good pedicel is cut into 5cm long sections, each section has an axillary bud, the bracts on the axillary bud are peeled off, first soaked in 75wt% alcohol solution, dry the alcohol with filter paper, then put in 0.1wt% Sterilize in a mercuric chloride solution with a high concentration for 8 minutes, and finally rinse with sterile water for 3 times, blot the water with filter paper and place it in a petri dish.

[0020] Cut off 0.5cm on both sides of the flower stalk, then inoculate the bud point upwards obliquely in the bud induction medium, the inoculation amount is 0.1g / ml 培养基 , cultured for 30 days at a temperature of 23°C and a light intensity of 3000Lux, and the composition of the bud induction ...

Embodiment 2

[0024] Select the peduncle of the mature mother plant of Phalaenopsis "Giant Treasure Red Rose" that is blooming as an explant, wash it with washing powder water, and then rinse it under running water for 30-40 minutes. The pedicel of the flower is cut into 5cm long sections, each section has an axillary bud, the bracts on the axillary bud are peeled off, first soaked in 75wt% alcohol solution, dry alcohol with filter paper, then put into 0.1wt% concentration Sterilize in the mercuric chloride solution for 10 minutes, and finally rinse with sterile water 3 to 4 times, blot the water with filter paper and place it in a petri dish.

[0025] Cut off 0.5cm on both sides of the flower stalk, then inoculate the bud point upwards obliquely in the bud induction medium, the inoculation amount is 0.2g / ml 培养基 ,, cultured for 40 days under the conditions of temperature 27°C and light intensity 3000Lux, the composition of the bud induction medium is Huabao No. 1 3.5g·l -1 +BA 3mg·l -1 +N...

Embodiment 3

[0029] Select the flower stalks of the mature mother plant "Red Prince" of Phalaenopsis with fully open flower buds as explants, wash them with washing powder water, and then rinse them under running water for 30-40 minutes. Cut into 5cm long sections, each section has an axillary bud, peel off the bracts on the axillary bud, first soak it in 75wt% alcohol solution, dry the alcohol with filter paper, then put 0.1wt% concentration of Sterilize in mercury solution for 12 minutes, and finally rinse with sterile water 3 to 4 times, blot the water with filter paper and place it in a petri dish.

[0030] Cut off 0.5cm on both sides of the flower stalk, then inoculate the bud point upwards obliquely in the bud induction medium, the inoculation amount is 0.1g / ml 培养基 ,, cultured for 30 days under the conditions of temperature 23°C and light intensity 3000Lux, the composition of the bud induction medium is Huabao No. 1 2.5g·l -1 +BA 2mg·l -1 +NAA 0.3mg·l -1 + 20g sucrose l -1 + Bana...

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Abstract

The invention relates to a vegetative propagation method for cattleya, in particular to a vegetative propagation method for butterfly orchid. The vegetative propagation method for butterfly orchid, provided by the invention, comprises the following steps of: by taking pedicel and axillary bud of butterfly orchid as explant, inoculating the explant into a culture medium for culturing; and performing subculture, rooting culture and transplanting culture on the induced new buds in turn. According to the method provided by the invention, the tillering of the butterfly orchid is realized in an artificially prepared culture medium; the single-stalk reproduction feature of the butterfly orchid is broken through; an induced pedicel seedling can be obtained without hurting a butterfly orchid stock plant according to the method; and the pedicel of the same stock plant can be repeatedly used. The seedlings produced according to the method are robust, trim and consistent; the source problem of excellent butterfly orchid seedlings during production is solved; and more economic and social benefits can be created.

Description

technical field [0001] The present invention relates to a kind of asexual reproduction method of Cayenopsis orchid, in particular to the asexual reproduction method of Phalaenopsis orchid. Background technique [0002] Phalaenopsis (Phalaenopsis sp.) is a perennial evergreen epiphytic herbaceous plant belonging to the family Phalaenopsis. It belongs to tropical aerial orchids and is easy to cultivate. "Reputation, it is one of the most popular western orchids in recent years. Phalaenopsis is a single-stem aerial orchid. The plant itself does not tiller and rarely develops side buds. Due to the particularity of its flower structure, pollination cannot be completed under natural conditions, so it is difficult to obtain seeds; artificial pollination is used to obtain seeds, and the germination of seeds The rate is also only about 20%. Inducing its seed germination on the artificial culture medium has opened up a new rapid propagation approach for the development of the Phalaen...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 吴海红赵兴华苏胜举印东生潘百涛胡新颖李丹邢桂梅屈连伟杨佳明崔玥晗姚园媛王茹
Owner 吴海红
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