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Wide-spectrum sterile sowing culture medium II for tropical orchids

A technology of aseptic sowing and culture medium, which is applied in the direction of culture medium, soilless cultivation, planting substrate, etc., and can solve the problems of inability to germinate seeds, poor germination rate, and poor broad-spectrum

Inactive Publication Date: 2017-08-18
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently disclosed medium has the defect of poor broad-spectrum. Generally, each tropical orchid corresponds to a kind of sowing medium, and the seeds of other types of orchid sowing medium cannot germinate or have a low germination rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0010] Broad-spectrum tropical orchid aseptic seeding medium two of the present invention, its preferred embodiment is:

[0011] Each L medium includes:

[0012] NH 4 NO 3 120g, KNO 3 34g, CaCl 2 2H 2 O 440g, MgSO 4 ·7H 2 O 370g, KH 2 PO 4 90g, 20g of sucrose, 2g of activated carbon, 5.8g of agar, and the rest are trace elements, organic components and iron salts of 1 / 4MS, pH=5.7.

[0013] Cultivate at the stage of mature fruit pods: take uncracked fruit pods and take them when the stalks of the fruit pods start to turn yellow.

[0014] The culture temperature is 24 to 26 ° C, and the light intensity is 60 μmol m -2 the s -1 , light time 12 hours / day.

[0015] The embodiments of the present invention will be further described in detail below.

[0016] Harvesting period of mature fruit pods: when the fruit pods are not cracked and the stalks of the fruit pods start to turn yellow.

[0017] Medium: NH 4 NO 3 120g / L+KNO 3 34g / L+CaCl 2 2H 2 O 440g / L+MgSO 4 ·...

specific Embodiment

[0020] (1) The fruit pods used for aseptic sowing are better when they are just mature but not yet cracked. Generally, it is better when the stalk of the fruit pods starts to turn yellow. At this time, the seeds in the capsule can be separated from each other and are easy to sow. Immature seeds stick together when sown, which is not conducive to later growth. In addition, uncracked fruit pods are easy to sterilize, and only the pod skin can be sterilized; after the fruit pods are cracked, it is more difficult to sterilize the powdered seeds, and the germination ability of the seeds is reduced. It is advisable to pick and sow the fruit pods immediately after harvesting. When it is time to sow in the future, they can be stored in a sealed plastic bag and stored in a refrigerator at 4°C, but the time should not be too long, otherwise it is easy to grow mold or reduce the germination rate.

[0021] (2) Sterile seeding must be carried out on the ultra-clean workbench. Prepare all ...

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Abstract

The invention discloses a wide-spectrum sterile sowing culture medium II for tropical orchids. Each L of culture medium contains the following components by weight: 120g of NH4O3, 34g of KNO3, 440g of CaCl2.2H2O, 370g of MgSO4.7H2O, 90g of KH2PO4, 20g of saccharose, 2g of activated carbon, 5.8g of agar and the balance of 1 / 4MS microelements, organic components and iron salt; and the pH value of the culture medium is 5.7. The tropical orchids are cultured by the following steps: carrying culturing in a mature fruit pod period; and picking un-cracked fruit pods when fruit stems of the fruit pods starting to yellow, wherein the culture temperature is 24-26 DEG C, the illumination intensity is 60 micro mol.m / 2s<-1>, and the illumination time is 12 hour / day. By utilizing the culture medium, the germination rate of paphiopedilum seeds is above 70%, the germination rates of cattleya, butterfly orchid and dendrobium seeds are above 90%, and the germination rates of other tropical orchid seeds are above 80%.

Description

technical field [0001] The invention relates to a flower culture medium, in particular to a broad-spectrum tropical orchid aseptic sowing medium II. Background technique [0002] Orchid is one of my country's top ten famous flowers and one of the world's famous and precious flowers. It occupies a very important position in flower production. With the rapid development of the economy, the acceleration of urbanization and the improvement of people's living standards, traditional orchids can no longer meet people's growing aesthetic needs. It has become the darling of flowers in the market because of its extraordinary beauty, changeable flower shape, long flowering period and rich and colorful colors. [0003] A large number of seedlings can be obtained by aseptic sowing, which is the main means of cross breeding to cultivate new varieties. However, the currently disclosed medium has the defect of poor broad-spectrum. Generally, each tropical orchid corresponds to a seeding m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G31/00
CPCA01G24/00C05B7/00C05G3/00C05C1/00C05C5/02C05D3/00C05D5/00C05D9/00C05D9/02C05F11/00
Inventor 郑宝强王雁
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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