Asepsis sowing and tissue culturing technique of Cattleya bowringiana Hort.

Abstract

The invention provides the rapid propagation technology of Cattleya seedlings. Cattleya is known as the king of orchids, with beautiful flower shapes, bright colors and high ornamental value. The trade volume in the international market is huge, and it may also become a new tropical orchid sales hotspot after Phalaenopsis and Whelan orchid in the Chinese market. However, the existing conventional production technology adopts solid medium for seeding, which takes a long time to germinate and grows slowly; the operation is labor-intensive, the cultivation time is long, and the cost is high. The purpose of the present invention is to improve the production process and use liquid culture in the early stage to promote the rapid germination and growth of seeds, and then directly inoculate the protocorms on the seedling medium to form seedlings in one step, reducing intermediate links, greatly reducing costs and cultivation time, The technology can be applied to the seedling production and breeding of hybrids within the genus Cattleya and close genera, and has a good application prospect.

Description

Technical field [0001] The invention provides a rapid propagation technology for the seedlings of Cattleya. Background technique [0002] Cattleya is known as the king of orchids. There are large-flowered varieties, mini-types, beautiful flower types, colorful colors, and high ornamental value. It can be used for potted plants or hanging pots, and it is also widely used for cut flowers. The trade volume in the international market is huge, and it may become a new tropical orchid sales hotspot after Phalaenopsis and Whelan in our market. [0003] Most of the commercial seedlings of Cattleya are seedlings. The current production process is: [0004] Seed germination medium (mother bottle) → growth and proliferation medium (middle mother bottle) → seedling medium [0005] On the solid germination medium, it takes 2 months to half a year from seed germination to subculture transfer bottle depending on the variety. After the seedlings are formed in the middle mother bottle, they are t...

AI Technical Summary

Problems solved by technology

[0005] It takes 2 months to half a year from seed germination to subculture on solid germination medium depending on the variety. After the seedlings are formed in the mother bottle, they are then transferred to the seedling medium for strong seedlings to take root. Need to spend a lot of labor and training costs

[0006] Cattleya can be propagated by division, but the propagation speed is slow, and Cattleya seeds are extremely difficult to germinate under natural conditions due to incomplete embryo development.

A large amount of test-tube seedlings can be obtained by using the method of test-tube aseptic sowing, but the existing conventional production technology uses solid medium for sowing, and the germination time of the seeds is long and the growth is slow; For seedling cultivation, after the seeds germinate, they need to go through the mother bottle stage, which is labor-intensive to operate, takes a long time to cultivate, and costs high