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Method for screening tulasnella fungi and establishing symbiotic system for promoting seed germination of cymbidium mannii and cattleya hybrida

A symbiotic system and a technology for establishing a method, applied in the biological field, to achieve the effect of improving the germination rate

Active Publication Date: 2018-05-18
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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Problems solved by technology

[0019] At present, in the prior art, there is no report on the screening of the fungus of the genus Colloidal fungus that can efficiently promote the germination of Cattleya and Sclerophylla simultaneously and the method for establishing the corresponding symbiotic system

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  • Method for screening tulasnella fungi and establishing symbiotic system for promoting seed germination of cymbidium mannii and cattleya hybrida
  • Method for screening tulasnella fungi and establishing symbiotic system for promoting seed germination of cymbidium mannii and cattleya hybrida

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[0038] 1. Test material

[0039] (1) Plant material: seeds of two species (YK, YD) of Cymbidium mannii and Cattleya hybrida.

[0040] (2) Fungal materials: 7 strains of mycorrhizal fungi (Tulasnellas pp.) of the genus Orchidaceae obtained from experiments in our laboratory for many years, see Table 1.

[0041] 2. Test method (symbiotic germination test of Orchidaceae plant seeds and fungus of the genus Colloides)

[0042] (1) Configuration of culture medium

[0043] The configuration of symbiotic medium (oat medium OMA): take oatmeal and grind it into powder, take three gradients of oat flour respectively, and prepare 0.5g / L, 1.0g / L, 2.0g / L, 3.0g / L , 4.0g / L, 8.0g / L, 12.0g / L OMA solutions. Boil in deionized water for 1 hour, filter, add 8.0 g of agar, add deionized water to the filtrate to 1000 mL, and sterilize at high temperature.

[0044] The configuration of the fungal culture medium: 200g peeled potatoes, cut into small pieces of 0.5-1cm square, add 800mL of deionized ...

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Abstract

The invention discloses a method for screening tulasnella fungi and establishing a symbiotic system for promoting seed germination of cymbidium mannii and cattleya hybrida. A Tcs1 bacterial strain isadopted and establishes a symbiotic relation together with one or more orchid seeds including cymbidium mannii seeds, cattleya hybrid YK seeds and cattleya hybrid YD seeds to promote germination of orchid seeds. A symbiotic germination test is carried out on an artificial medium through adoption of tulasnella fungi and orchid seeds, and is compared with a control group of orchid seeds with no fungi inoculated to screen mycorrhizal fungi capable of widely efficiently promoting seed germination and to screen appropriate symbiotic mediums. Efficient symbiotic fungi for a germination stage of orchid seeds are obtained, the germination rate of orchid seeds is improved via symbiotic germination culture technique, and a symbiotic system of orchid and mycorrhizal fungi is established.

Description

technical field [0001] The present invention relates to a kind of biotechnology, relate in particular to a kind of screening and the establishment method of symbiosis system of the colloidal fungi that effectively promote the germination of Sclerophylla and Cattleya seeds. Background technique [0002] Orchidaceae (Orchidaceae) belongs to the monocotyledonous class of angiosperms, and there are about 700 genera and more than 35,000 species distributed in the world (Dressler, 1993; Cribb, 2003), and its number of species ranks second only to Compositae Branch (Lang Kaiyong, 1994). It is beautiful in shape, bright in color, and has great ornamental and medicinal value. [0003] Orchids have two notable characteristics: [0004] 1. The embryo of Orchidaceae seeds is only composed of dozens of cells, which is incompletely differentiated and difficult to germinate naturally. [0005] 2. Orchidaceae are almost all mycorrhizal plants. In the natural growth state, no matter green...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/04A01C1/00A01G18/10C12R1/645
CPCA01C1/00C12N1/14C12Q1/04
Inventor 李潞滨刘蕾魏莉王涛杨前宇
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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