Asepsis sowing and tissue culturing technique of Cattleya bowringiana Hort.

A technique for aseptic seeding and tissue culture, applied in botany equipment and methods, horticulture, gardening tools/equipment, etc., can solve the problems of large labor and cultivation costs, labor-intensive operations, and high costs, and achieve good application prospects, The effect of high output and low investment

Inactive Publication Date: 2005-06-29
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It takes 2 months to half a year from seed germination to subculture on solid germination medium depending on the variety. After the seedlings are formed in the mother bottle, they are then transferred to the seedling medium for strong seedlings to take root. Need to spend a lot of labor and training costs
[0006] Cattleya can be propagated by division, but the propagation speed is slow, and Cattleya seeds are extremely difficult to germinate under natural conditions

Method used

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Examples

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Effect test

example 1

[0019] 1. Materials: Pollination of Cattleya bowringiana var.coerulea (Sapphire Cattleya) 3 days after flowering, and uncracked fruit pods for sowing 180 days after pollination.

[0020] 2. Fruit pod disinfection: soak the fruit pods in 75% alcohol for 30 seconds, put them in 0.1% mercuric chloride solution for 20 minutes, and rinse them with sterile water for 5 times.

[0021] 3. Seed liquid culture: cut the fruit pods and inoculate the white powdery seeds into the seed germination liquid medium (improved V&W medium+6-benzylpurine 0.5 mg / liter+naphthalene acetic acid 0.5 mg / liter+10% coconut milk ) in 150rpm shaker; the embryos germinate in about a week, and form green protocorms with a diameter of 1-2mm in about 20 days.

[0022] 4. Seedling cultivation: inoculate the protocorm formed in the liquid medium for 25 days with a straw into the seedling medium (Hyponex 1 3 g / L+banana juice 100 g / L+6-benzyl base purine 1 mg / L + naphthalene acetic acid 0.5 mg / L + activated carbon 1...

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Abstract

The invention provides a rapid breeding method of Cattleya by improving the current flow of production, the method comprises fluid culturing on earlier stage, so as to promote the seed to grow fast, then directly inoculating the original bulbodium to the sprout culture medium for sprout production in one step. The method can reduce culture cost and time greatly, and can be applied to Catteva genus, near source bigeneric hybid seedling production and breeding works.

Description

technical field [0001] The invention provides the rapid propagation technology of Cattleya seedlings. Background technique [0002] Cattleya is known as the king of orchids. There are large flower strains and miniature strains, with beautiful flower shapes, bright colors and high ornamental value. It can be used for potted plants or hanging pots, and is also widely used for cut flowers. The trade volume in the international market is huge, and it may also become a new tropical orchid sales hot spot in the Chinese market after Phalaenopsis and Cymbidium phalaenopsis. [0003] Most of the Cattleya commercial seedlings are seedlings, and the current production process is as follows: [0004] Seed germination medium (mother bottle) → growth and proliferation medium (mother bottle) → seedling growth medium [0005] It takes 2 months to half a year from seed germination to subculture on solid germination medium depending on the variety. After the seedlings are formed in the moth...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 段俊曾宋君陈之林黄向力温铁龙
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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