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A kind of symbiosis system establishment method of the fungi of the genus Colloidal fungus that promotes the germination of sclerophylla and cattleya

A symbiotic system, a technology for establishing a method, applied in the biological field to achieve the effect of improving the germination rate

Active Publication Date: 2021-01-08
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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Problems solved by technology

[0019] At present, there are no reports in the prior art on the screening of fungi of the genus Colloidal fungi that can simultaneously efficiently promote the germination of Cattleya and Sclerophyllum seeds and the establishment of a corresponding symbiotic system

Method used

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  • A kind of symbiosis system establishment method of the fungi of the genus Colloidal fungus that promotes the germination of sclerophylla and cattleya
  • A kind of symbiosis system establishment method of the fungi of the genus Colloidal fungus that promotes the germination of sclerophylla and cattleya

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[0039] 1. Test material

[0040] (1) Plant material: seeds of two species (YK, YD) of Cymbidium mannii and Cattleya hybrida.

[0041] (2) Fungal materials: 7 strains of mycorrhizal fungi (Tulasnellas pp.) of the genus Orchidaceae obtained from experiments in our laboratory for many years, see Table 1.

[0042] 2. Test method (symbiotic germination test of Orchidaceae plant seeds and fungus of the genus Colloides)

[0043] (1) Configuration of culture medium

[0044] The configuration of symbiotic medium (oat medium OMA): take oatmeal and grind it into powder, take three gradients of oat flour respectively, and prepare 0.5g / L, 1.0g / L, 2.0g / L, 3.0g / L , 4.0g / L, 8.0g / L, 12.0g / L OMA solutions. Boil in deionized water for 1 hour, filter, add 8.0 g of agar, add deionized water to the filtrate to 1000 mL, and sterilize at high temperature.

[0045] The configuration of the fungal culture medium: 200g peeled potatoes, cut into small pieces of 0.5-1cm square, add 800mL of deionized ...

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Abstract

The invention discloses a method for screening tulasnella fungi and establishing a symbiotic system for promoting seed germination of cymbidium mannii and cattleya hybrida. A Tcs1 bacterial strain isadopted and establishes a symbiotic relation together with one or more orchid seeds including cymbidium mannii seeds, cattleya hybrid YK seeds and cattleya hybrid YD seeds to promote germination of orchid seeds. A symbiotic germination test is carried out on an artificial medium through adoption of tulasnella fungi and orchid seeds, and is compared with a control group of orchid seeds with no fungi inoculated to screen mycorrhizal fungi capable of widely efficiently promoting seed germination and to screen appropriate symbiotic mediums. Efficient symbiotic fungi for a germination stage of orchid seeds are obtained, the germination rate of orchid seeds is improved via symbiotic germination culture technique, and a symbiotic system of orchid and mycorrhizal fungi is established.

Description

technical field [0001] The invention relates to a biological technology, in particular to a method for establishing a symbiotic system of a fungus belonging to the genus Colloides which effectively promotes the germination of sclerophyllum and cattleya seeds. Background technique [0002] Orchidaceae (Orchidaceae) belongs to the class Monocots of the angiosperm phylum, and there are about 700 genera and more than 35,000 species distributed in the world (Dressler, 1993; Cribb, 2003), and its number of species ranks second only to Compositae. Branch (Lang Kaiyong, 1994). It is beautiful in shape, bright in color, and has great ornamental and medicinal value. [0003] Orchids have two notable characteristics: [0004] 1. The embryo of Orchidaceae seeds is only composed of dozens of cells, which is incompletely differentiated and difficult to germinate naturally. [0005] 2. Orchidaceae are almost all mycorrhizal plants. In the natural growth state, no matter green leaf orchi...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12Q1/04A01C1/00A01G18/10C12R1/645
CPCA01C1/00C12N1/14C12Q1/04
Inventor 李潞滨刘蕾魏莉王涛杨前宇
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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