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Cattleya primary induction medium and explant pretreatment method

A technology for inducing culture medium and Cattleya, applied in the directions of botanical equipment and methods, tissue culture, horticultural methods, etc., can solve problems such as browning of explants, reduce the degree of browning, promote germination, slow down oxidative enzymatic effect of reaction

Active Publication Date: 2016-05-04
徐州大成环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on this, it is necessary to provide a Cattleya first-generation induction medium that can effectively reduce the browning of Cattleya explants for the problem of explant browning in the process of Cattleya tissue culture.
[0006] In addition, it is necessary to provide a Cattleya explant pretreatment method that can effectively reduce the browning of Cattleya explants for the problem of explant browning during Cattleya tissue culture

Method used

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  • Cattleya primary induction medium and explant pretreatment method
  • Cattleya primary induction medium and explant pretreatment method
  • Cattleya primary induction medium and explant pretreatment method

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment one: the preparation of Cattleya first generation induction medium of the present invention

[0022] According to the formula shown in Table 1, each component of the improved MS medium was weighed, placed in a conical flask, an appropriate amount of purified water was added, stirred and dissolved, and the volume was adjusted to 1 L to prepare the improved MS medium for later use.

[0023] Table 1 Composition and dosage of improved MS medium

[0024]

[0025]

[0026] According to the formula shown in Table 2, citric acid, salicylic acid, lanthanum nitrate, 6-BA, NAA, agar powder and sucrose were added to the prepared modified MS medium, stirred to dissolve, and the pH value was adjusted to 5.5 . Then seal the triangular flask, place it in an autoclave, and sterilize it at 121° C. for 20 minutes, and then place it in an ultra-clean workbench to cool naturally to obtain a sterilized culture medium mother solution for future use.

[0027] In the ultra-cl...

Embodiment 2

[0030] Embodiment two: Cattleya explant pretreatment method of the present invention

[0031] Cut 1-2cm lateral buds from healthy and well-growing Cattleya, rinse them with detergent, and treat them at a low temperature of 2-5°C for 12-24 hours; take out the lateral buds after low-temperature treatment in an ultra-clean workbench, and remove the lateral buds Leave a leaf on the outer bracts and lateral buds, scrub with 70% alcohol for a few seconds, rinse with sterile water, sterilize in 0.1% mercuric chloride solution for 5 to 10 minutes, and then use sterile Rinse with bacterial water; cut lateral buds with a diameter of 0.5-2mm in a petri dish and set aside.

[0032] In the ultra-clean workbench, inoculate the sterilized lateral buds into the Cattleya first-generation induction medium prepared in Example 1, then place the tissue culture bottle in an artificial climate box for cultivation, and the environmental parameters of the artificial climate box The settings are: temp...

Embodiment 3

[0033] Embodiment three: Cattleya explant browning rate, survival rate, germination rate test

[0034]With reference to the Cattleya explant pretreatment method described in Example 2, the Cattleya lateral buds were inoculated in the primary induction medium shown in Table 3 respectively, and the browning rate, germination rate and survival rate of the lateral buds were recorded respectively after cultivating for 20 days , after 60 days of cultivation, the germination rate was counted respectively, and the results are shown in Table 4.

[0035] Table 3 Cattleya primary induction medium

[0036]

[0037] Remarks: The above-mentioned control group 1, control group 2 and each test group all used the improved MS medium shown in Table 1 as the basal medium; the control group 3 used 1 / 4 MS medium as the basal medium, and added 0.2% Activated carbon; control group 2 and control group 3 refer to the explant treatment method described in Example 2, but do not carry out low temperat...

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Abstract

The invention discloses a primary cattleya hybrida inducing medium and an explant pretreatment method. The primary cattleya hybrida inducing medium takes an improved MS culture medium as a basic culture medium; citric acid, salicylic acid, lanthanum nitrate, 6-BA, NAA, agar powder, cane sugar and silica gel are added into the basic culture medium; the browning of the cattleya hybrida explants can be effectively alleviated.

Description

technical field [0001] The invention relates to the technical field of orchid tissue culture, in particular to a first-generation Cattleya induction medium and an explant pretreatment method. Background technique [0002] Cattleya Hybrida, also known as Cattleya Hybrida, is known as the "King of Cattleya Hybrida" because of its large flowers, bright colors, rich aesthetic feeling, and many varieties, some of which have aroma. Appreciate value and economic value. [0003] Cattleya can be propagated by seed, division and tissue culture. Because Cattleya seeds are extremely difficult to germinate under natural conditions, the reproduction rate of the division method is low, and it is difficult to achieve the purpose of large-scale production, and tissue culture is currently the main method for large-scale production of Cattleya. However, the explants of Cattleya are prone to browning and necrosis during tissue culture, which is a major obstacle to the success of protocorm ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
Inventor 陈永得
Owner 徐州大成环境科技有限公司
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