Method for fast breeding allium ampeloprasun var.ampeloprasun

An elephant and garlic technology, applied in the field of rapid reproduction of elephant garlic, can solve the problems such as being unsuitable for the rapid reproduction of elephant garlic, different biological learning, and achieve the advantages of low cost of ingredients, improved reproduction coefficient, and high transplant survival rate. Effect

Inactive Publication Date: 2008-08-06
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Since elephant garlic and ordinary garlic belong to the Allium family of Liliaceae, we refer to the technology of garlic rapid propagation in the process of tissue culture and rapid propagation of elephant garlic, but after all, elephant garlic and ordinary garlic are biologically different. There is a big difference, the rapid propagation method using the existing common garlic is no longer suitable for the rapid propagation of elephant garlic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Materials: Elephant garlic (Allium ampeloprasun var.ampeloprasun), provided by Science and Technology Bureau of Cangshan City, Shandong Province, purchased from the United States.

[0034] A method for rapidly propagating elephant garlic, the steps comprising explant disinfection, induction of sterile seedlings, induction of adventitious buds and subculture proliferation, rooting of tissue cultured seedlings, transplanting of tissue cultured seedlings, is characterized in that it includes the following steps:

[0035] A. Disinfection of explants

[0036] Select the bulbs of elephant garlic as explants, soak them in a solution with 10% by weight of tap water for 5 to 10 minutes, rinse them under tap water, and carry out surface disinfection on an ultra-clean workbench;

[0037] On the ultra-clean workbench, use 75% alcohol to disinfect the surface for 1 minute, then rinse with sterile water for 1 to 2 times, then disinfect with 15% sodium hypochlorite solution for 10 to ...

Embodiment 2

[0064] Present embodiment material is with embodiment 1.

[0065] The operating method, sterile seedling induction medium, rooting medium, and tissue culture room culture conditions of this embodiment are the same as those in Example 1.

[0066] The adventitious bud induction and proliferation medium described in this embodiment is based on MS basic medium, adding 1.0 mg / L of 6-benzyl adenine 6-BA, 1.0 mg / L of kinetin KT, and indole butyric acid IBA1.0mg / L (abbreviated as: MS+6-BA 1.0mg / L+KT1.0mg / L+IBA1.0mg / L);

[0067] No vitrification phenomenon was observed in the apical meristem described in step B during the induction of the sterile vaccine.

[0068] No vitrification phenomenon was observed in the base of the sterile seedlings and the base of adventitious buds described in step C during the induction of adventitious buds and subculture.

[0069] In this embodiment, the multiplying rate is 3.5, the rooting rate is 100%, and the survival rate is 100%.

[0070] In the pre...

Embodiment 3

[0072] Present embodiment material is with embodiment 1.

[0073] The operating method, sterile seedling induction medium, rooting medium, and tissue culture room culture conditions of this embodiment are the same as those in Example 1.

[0074] The adventitious bud induction and proliferation medium described in this example is based on the 1 / 2 MS medium obtained by reducing the macroelements in the MS basic medium components to 1 / 2 of the original and keeping other components unchanged, and adding α - Naphthaleneacetic acid NAA 0.1mg / L, indole butyric acid IBA 1.5mg / L (abbreviated as: 1 / 2MS+NAA0.1mg / L+IBA 1.5mg / L);

[0075] No vitrification phenomenon was observed in the apical meristem described in step B during the induction of the sterile vaccine.

[0076] No vitrification phenomenon was observed in the base of the sterile seedlings and the base of adventitious buds described in step C during the induction of adventitious buds and subculture.

[0077] In this embodiment...

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PUM

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Abstract

The invention provides a method for rapid propagation of Allium ampeloprasun var.ampeloprasun. The invention adopts an adventitious bud proliferation method to rapidly propagate Allium ampeloprasun var.ampeloprasun, makes use of apical meristem to get aseptic seedling and then makes use of root of the aseptic seedling to obtain a plurality of proliferatively adventitious buds. The invention avoids vitrification of tissue culture seedling resulted from dedifferentiation and redifferentiation during tissue culture and greatly improves propagation coefficient. The invention comprises the operation steps of disinfection of explant, aseptic seedling induction, adventitious bud induction, subculture multiplication, adventitious bud rooting and tissue culture seedling transplantation. The invention takes MS basic medium as a basis and adds plant hormone (Alpha-NAA, IBA, 6-BA, KT and GA) of different proportions in different combinations to reproduce new plants rapidly on an appropriate adventitious bud induction, multiplicated medium and a rooting medium. The method for rapid propagation of Allium ampeloprasun var.ampeloprasun of the invention has convenient operation, low cost and high application value.

Description

technical field [0001] The invention belongs to the field of regenerated plants obtained by tissue culture technology, in particular to a method of rapidly multiplying elephant garlic (Allium ampeloprasun var.ampeloprasun) method. Background technique [0002] It has a history of more than 2,000 years since garlic was introduced to China by Zhang Qian in the Western Han Dynasty when he was sent to the Western Regions, and he brought garlic back to the country to settle down. Garlic is an indispensable seasoning in human daily life. It can remove fishy smell and increase flavor when cooking fish, meat, poultry and vegetables. Especially in cold dishes, it can not only increase flavor, but also sterilize. There are many varieties of garlic, which can be divided into purple garlic and white garlic according to the color of the bulb skin. Purple garlic has few and large garlic cloves, strong pungent taste and high yield. It is mostly distributed in North China, Northwest and N...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 张秀海梁宏霞黄丛林王永勤吴忠义李春华
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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