Industrial horseradish planting method

A planting method and factory-like technology, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve problems such as difficulty in producing a large number of high-quality healthy seedlings, low seedling reproduction coefficient, and susceptibility to ink infestation, etc. Achieve the effect of improving factory production capacity, low utilization rate, and labor reduction

Active Publication Date: 2013-06-05
四川华阳蓝公府实业有限公司
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Problems solved by technology

[0005] It can be seen from the above that the traditional seedling breeding method leads to low seedling reproduction coefficient and slow propagation speed, it is difficult to prod

Method used

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  • Industrial horseradish planting method
  • Industrial horseradish planting method

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Embodiment

[0053] (A1) Germination: Select the wasabi variety that needs to be cultivated, keep the seeds at 18°C-22°C, and the humidity is 90%, to promote their germination;

[0054] (A2) Material selection: when the germination length of wasabi seeds is 1-2cm, cut off the stem tip, the length is 0.3-0.4cm;

[0055] (A3) Cleaning: After cleaning the buds sorted in step (A2) with washing powder, wrap them in gauze, rinse them with tap water for 2 hours, then soak them in 1% solution of Xiaoxiling for 30 minutes, and finally wash them with non-toxic Bacteria moisture-absorbing paper basically absorbs moisture;

[0056] (A4) Sterilization: Soak the wasabi sprout stem tip prepared in step (A3) with 70% alcohol by volume for 28-32 seconds, then disinfect it with 0.2% mercury liter, and finally wash it with sterile water for 5 times ;

[0057] (A5) First-generation cultivation: use culture medium to cultivate sterile wasabi seedlings from the sterilized shoot tips;

[0058] (A) Rooting cul...

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Abstract

The invention discloses a horseradish seedling culturing method. The horseradish seedling culturing method comprises the following steps: (A) rooting cultivation, (B) hardening seedling transplanting and (C) filed planting, wherein the rooting cultivation comprises the step that primarily cultured stem tip meristem tissue which undergoes sterilization and detoxification is transferred into a 1/2 Murashige and Skoog (MS) culture medium to grow, the hardening seedling transplanting comprises the step of transferring the tissue into a tray after the step (A) lasts for 28-32 days, and the field planting comprises the step of transplanting finished horseradish seedlings to fields after the step (B) lasts for 75-85 days. The method of adding rooting agents into the culture medium for enrichment culture is adopted to replace the method of using cytokinin, so that the problems that rooting is slow and roots are thin and long are solved, and the purpose of increasing the length of the root of a single seedling quickly in a short period is achieved. At the same time, the price of the rooting agents is far lower than that of the cytokinin. Compared with the auxin culturing method which is large in waste quantity and low in use ratio, the horseradish seedling culturing method is smaller in dosage, higher in use ratio and capable of greatly reducing cost.

Description

technical field [0001] The invention relates to a method for planting wasabi seedlings in the field of agriculture, in particular to a planting method for industrialized wasabi. Background technique [0002] In the development of wasabi industry in our country, ink disease (also known as black heart disease) is the most important disease of wasabi planting, which can cause complete failure of wasabi planting in severe cases. Existing studies have found that squirrel disease is a specific disease of wasabi, which does not cause harm to other crops and does not cause adverse effects on human health. Ink disease is caused by the fungus Phoma wasabiae Yokogi invading wasabi plants and infecting wasabi plants mainly through vascular bundle conduction, which seriously affects the quality and yield of wasabi, and even leads to the failure of wasabi harvest. Ink disease can damage the rhizomes, fibrous roots, leaves, and petioles of wasabi. After many years of research in Japan, T...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G1/00
Inventor 方季虎
Owner 四川华阳蓝公府实业有限公司
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