The invention discloses a camellia oleifera seedling radicle grafting method which comprises the steps of camellia oleifera seed screening and sowing, nursery land selection and preparation, nutrient soil and container preparation, grafted scion collection and treatment, determination of proper grafting time, treatment of rootstock seedlings, radicle cutting, radicle grafting and container seedling management. Compared with the prior art, the method has the greatest difference that the radicles of camellia oleifera rootstock seedlings are used as grafting points and are subjected to multi-section cutting to obtain multi-section standard rootstocks, the number and quality of the grafted rootstocks are improved, the grafting cost is reduced, the standard rootstock seedlings are soaked in a plant growth regulator, the activity of peroxidase (POD) and polyphenol oxidase (PPO) is improved, callus formation is promoted. elongation growth of roots and branching are facilitated, and the survival rate of grafted seedlings is increased. The camellia oleifera seedling radicle grafting method has the advantages of being low in cost, high in grafting success rate, free of sprouts, high in improved variety purity, high in survival rate, convenient to construct and operate, capable of saving manpower and material resources and the like.