45 results about "Arabidopsis seedling" patented technology

The invention discloses a method for optimizing a vitrification ultra-low temperature preservation effect of arabidopsis seedlings. According to the invention, the arabidopsis seedlings are processed by adopting a vitrification solution which contains a carbon nanometer material so as to improve the preservation effect of the arabidopsis seedlings. The method specifically comprises the steps of loading liquid treatment, vitrification solution treatment and liquid nitrogen preservation, wherein the vitrification solution is the MS culture solution containing 0.1-0.5 g/L of grapheme quantum dots. The method disclosed by the invention optimizes the preservation effect of the arabidopsis seedlings remarkably; as the grapheme quantum dots are added to serve as an allogenic material, the vitrification ultra-low temperature preservation of plants is facilitated.

The invention relates to the field of vitrification ultra-low temperature storage, in particular to a screening method of an allogenic material for promoting vitrification ultra-low temperature storage; an arabidopsis thaliana seedling is adopted as an experimental object; the allogenic material is added into vitrification solution for the vitrification ultra-low temperature storage of the arabidopsis thaliana seedling; the arabidopsis thaliana seedling which is not added with the allogenic material is adopted as a contrast; and the effect of the allogenic material is evaluated by comparing the recovery rate. According to the screening method of the allogenic material for promoting vitrification ultra-low temperature storage, the result is accurate, the repeatability is strong, the scope of application is wide, batch screening can be carried out, the efficiency is high, an effective approach is provided for screening the allogenic material, technical guarantee is provided for a vitrification ultra-low temperature storage technology, and a guiding role is played to the storage of plant species resources.

The invention provides a salt tolerant gene SRAT2 in arabidopsis thaliana, which has the base sequence stated in the sequence table SEQ ID No.1. A model plant arabidopsis thaliana is transformed by the salt tolerant gene SRAT2 in arabidopsis thaliana, so the salt tolerance in the arabidopsis thaliana seedling can be improved. If vegetables and flowers such as rice and strawberries are transformed by the salt tolerant gene SRAT2 in arabidopsis thaliana, the salt tolerance in the plants can be improved. On one hand, the yield of rice, outdoor vegetables and flowers in saline-alkali land can be improved and the application of the saline-alkali land in coastal areas of China can be improved; on the other hand, an obstacle of continuous cropping caused by soil salinization under the equipment condition can be overcome, so the yield and quality of plants such as vegetables and flowers are improved and the income of farmers is increased.

The invention discloses an application of arabidopsis microRNA400/arabidopsis microRNA400 precursor MIR400 in regulating and controlling cadmium resistance of plants/preparing transgenic plants, wherein the nucleotide sequence of arabidopsis microRNA400 is as shown in SEQ ID NO.1; the nucleotide sequence of arabidopsis microRNA400 precursor MIR400 is as shown in SEQ ID NO.2. According to the invention, MIR400 containing microRNA400 intron is cloned from arabidopsis, microRNA playing a role in regulating plant cadmium resistance in the seedling period of arabidopsis is verified. After verification, transgenic arabidopsis with higher cadmium resistance than a wild type can be obtained by reducing expression of microRNA. The microRNA in the invention can provide theoretic basis and gene sources for breeding new species of crops.

The invention discloses a device for screening and culturing of transgenic arabidopsis seedlings in situ, which comprises an incubator cover plate and a box body. A culture hole array is arranged on the incubator cover plate; a seedling tube is arranged inside a culture hole; and a shading board is arranged on a side wall of the box body and is movably connected with the box body. Compared with the traditional methods, the novel method has the following advantages that: 1) the novel method can screen T2-generation seeds of transgenic arabidopsis strains under the natural condition, does not need the sterile condition like the traditional methods, and is time-saving and labor-saving; 2) the novel method is an in-situ screening method, does not need to transfer the resistant seedlings from a sterile growing environment to a vermiculite or other growing environments like the traditional methods, and does not cause loss of the seedlings; and 3) the growth potential of the resistant seedlings screened by the novel method is better than that of the traditional methods, and transgenic pure lines (T3-generation seeds) are obtained early.

The invention discloses a protein related to fatty acid synthesis, an encoding gene and application thereof. The protein related to the fatty acid synthesis provided by the invention is called as GmLEC1A, is derived from soy beans and is the protein shown as 1) or 2): 1) the protein consists of amino acid sequences shown in a sequence 1 in a sequence table; and 2) the protein which is derived from the 1) by substituting and/or deleting and/or adding one or more amino acid residues of amino acid residue sequences in the sequence 1 of the sequence table and is related to vegetable fatty acid synthesis. After performing over-expression on the gene GmLEC1A protected by the invention in arabidopsis, the content of fatty acid in an arabidopsis seedling can be improved. Therefore, the GmLEC1A has important theoretical and practical significance for the improvement on the fatty acid content of the soy beans and the improvement on relative characters, and has board application and market prospects in the field of agriculture.

The invention discloses an experimental system for detecting the influence of rhizosphere microorganisms on plant growth and root development. The experimental system is characterized in that a two-grid culture dish is used as an experimental mold, sterile arabidopsis seedlings growing for 5 +/-1 days are transferred to a culture medium in the dish, rhizosphere bacterial liquid is directly inoculated on the root system of one grid of seedlings, the other grid of seedlings is used as a contrast, and sterile water of which the amount is equal to that of the bacterial liquid is added without inoculating bacteria; then the two-grid culture dish is sealed with a breathable pressure-sensitive adhesive tape, and is vertically placed in an illumination incubator, and culturing is performed for 4-6days; and whether inoculated microorganisms can promote plant growth and root system development or not is judged by observing and measuring the lengths of seedlings and main roots of the overgroundparts of plants and counting the density of lateral roots, or whether the microorganisms have tryptophan-dependent auxin synthetic pathways or not is identified. The system is helpful for screening and application of rhizosphere microorganisms for promoting plant growth and root development, and has important value for agricultural production and microorganism exploration.

The invention discloses an MYB transcription inhibition factor LrETC1 related to lycium ruthenicum anthocyanin synthesis and application thereof, and belongs to the technical field of gene engineering. According to the invention, the MYB transcription inhibition factor LrETC1 participating in the anthocyanin synthesis is screened and cloned, the total cDNA of the gene is 240bp, and 79 amino acids are encoded; protein sequence database analysis shows that the transcription factor belongs to R3 type MYB; and multiple sequence alignment and evolutionary tree analysis show that the transcription inhibition factor belongs to AtCPC-like transcription inhibition factors, and is a transcription factor which is located in a cell nucleus and does not have an activation function. A LrETC1 transgenic arabidopsis thaliana strain is obtained through agrobacterium tumefaciens-mediated genetic transformation, compared with a wild type, seed coats of seeds are light brown, arabidopsis thaliana seedlings are free of pigment accumulation under the stress condition of high sucrose concentration, and the expression level of related structural genes is remarkably reduced.

The invention discloses a protein derived from chinese wildrye and related to saltresistance and an encoding gene and application of the protein. The protein provided by the invention is (a) or (b), wherein (a) is a protein consisting of an amino acid sequence shown in the sequence 1 in a sequence table and (b) is a protein which is obtained by substituting and/or losing and/or adding one or more amino acid residues of the amino acid sequence shown in the sequence 1, is related to plant saltresistance and is derived from the sequence 1. The protein provided by the invention and the encoding gene of the protein can be used as candidate genes of plant saltresistance genetic engineering and used for improving the saltresistance of plants. Twenty one days after salt stress (200Mm NaCl) treatment, all LcMYB7342 gene modified Arabidopsis seedlings are normal in phenotype, but 78.6% of wild Arabidopsis seedlings have salt damage symptoms, get wilting and turn yellow. LcMYB7342 gene can be used for cultivating and identifying varieties of resistance plants which are needed in agriculture, husbandry and ecological environmental management and has a high practical application value and a bright application prospect in agriculture and husbandry.

The invention relates to the technical field of biological control of plant diseases, and discloses virus-carrying low-toxicity sclerotinia sclerotiorum and application thereof in biological control.The strain is sclerotinia sclerotiorum 767R45, and the preservation number is CCTCC NO: M20419. Volatile substances generated by the sclerotinia sclerotiorum 767R45 strain and 10% of fermentation filtrate of the volatile substances can inhibit the growth of sclerotinia sclerotiorum, rhizoctonia solani, botrytis cinerea, fusarium oxysporum, Bipolaris rice, leptosphaeria biglobosa and geotrichum citri-aurantii hyphae. Besides, the volatile substances and the fermentation filtrate generated by the sclerotinia sclerotiorum 767R45 strain can inhibit the occurrence of gray mold, penicilliosis and penicilliosis in the storage period, the fermentation filtrate can also inhibit the occurrence of sclerotinia sclerotiorum, and the volatile substances can promote the growth of arabidopsis seedlings. According to the invention, phytopathogens become beneficial biocontrol resources, and a new visual angle and a new means are provided for biological control of fungal diseases of crops.

The invention provides and discloses an Arabidopsis thaliana salt-tolerant gene SRAT1, which has the base sequence described in SEQ ID NO.1 in the sequence listing. Transforming the Arabidopsis thaliana salt-tolerant gene SRAT1 into the model plant Arabidopsis can improve the salt tolerance of Arabidopsis seedlings. If the Arabidopsis salt-tolerant gene SRAT1 is transformed into plants such as rice or strawberry and other vegetables and flowers, it is possible to improve the salt tolerance of plants. Utilization of saline-alkali land; on the other hand, it can overcome continuous cropping obstacles caused by soil back-salt under facility conditions, improve the yield and quality of vegetables, flowers and other plants, and increase farmers' income.

The invention discloses a protein GmLEC1B related to fatty acid synthesis, its encoding gene and application. The protein related to fatty acid synthesis provided by the present invention, named GmLEC1B, is derived from soybean (Glycine max L.), and is a protein of the following 1) or 2): 1) consists of the amino acid sequence shown in Sequence 1 in the sequence listing 2) The amino acid residue sequence of sequence 1 in the sequence listing undergoes one or several amino acid residue substitutions and/or deletions and/or additions and is related to plant fatty acid synthesis and derived from 1). After the gene GmLEC1B protected by the invention is overexpressed in Arabidopsis thaliana, the fatty acid content in Arabidopsis seedlings can be increased. This has important theoretical and practical significance for improving the fatty acid content of soybean and related traits, and has broad application and market prospects in the agricultural field.

The invention discloses a screening method of a banana water channel protein gene promoter core area. The screening method comprises the steps as follows: control selection: transgenic arabidopsis forconverting a CaMV35S promoter as a control; detection of promoter GUS activity: promoter GUS activity detection is performed on leaves and root systems of 15-day transgenic Arabidopsis seedlings treated by 100 mM, 200 mM and 300 mM 18% PEG 6000; and observation of staining condition: seen from phenotype, in GUS staining, except CaMV35S control plants, promoter transgenic plant leaves of each fragment is not stained or lightly stained while root tip parts are deeply stained in transgenic root systems. According to the result, the core area of MaPIP1:1 promoter is recognized, and the acting mechanism of the MaPIP1;1 gene during resisting of drought stress can be further illuminated.

The invention discloses an arabidopis thaliana AAP1 gene cloning and plant expression vector construction method, and the method is as follows: total RNA of an arabidopis thaliana seedling is extracted by a TRIzol method, the OD260 / OD280 ratio is determined to be 2.014; gel recovery of PCR (polymerase chain reaction) products is performed, recovered products are connected with a PMD-18T carrier, after conversion is completed, plasmid is extracted, PCR and enzyme digestion verification of the extracted plasmid is performed, a 1600bp DNA band is obtained by PCR verification of the plasmid; sequencing reveals that the gene ORF1458bp encodes a polypeptide of 485 amino acids, after respective enzyme digestion of a target gene and pCAMBIA3300 vector by use of restriction enzyme EcoR I and Xba I, the target gene is directionally connected to a plant expression vector, after conversion is completed, plasmid is extracted, and PCR and enzyme digestion verification of the extracted plasmid is performed; a 1600bp DNA band is obtained by PCR verification of the plasmid; by enzyme digestion verification, a 8300bp pCAMBIA3300 vector and a 1600bp target fragment are respectively obtained. The vector is successfully constructed, and is renamed P3300-AAP1.

The present invention relates to a salt-tolerant gene HgS3 isolated from a halophyte halogeton glomeratus, and applications thereof. A purpose of the present invention is to provide a new salt-tolerant gene HgS3 and an encoding protein thereof, and applications of the salt-tolerant gene HgS3 in improvement of the salt tolerance of plants and cultivation of new salt-tolerant varieties (lines). According to the present invention, the salt-tolerant gene HgS3 comprises the cDNA nucleotide sequence represented by SEQ ID NO.1, and has the molecular weight of 468 bp, the cDNA coding sequence is a site 208-site 336 nucleotide sequence represented by SEQ ID NO.1, the molecular weight is 129 bp, and the amino acid sequence is represented by SEQ ID NO.3, and comprises 42 amino acids; the salt-tolerant gene HgS3 can significantly improve the salt tolerance of transgenic Arabidopsis seedlings, and can contribute to the cultivation of new salt-tolerant plant (crop) varieties (lines).

The invention discloses a cultivating method of strong arabidopsis seedlings. The method includes the following steps that filter paper is put in a culture dish, after the filter paper is soaked in clean water, arabidopsis seeds are put on the filter paper, and the filter paper is put in a refrigerator to be vernalized for 2-4 days at the temperature of 3-4 DEG C; the vernalized arabidopsis seedsare sown in nutrition bowls with a seedling culture medium soaked in clean water with a toothpick; 5-6 arabidopsis seeds are sown in each nutrition bowl, the nutrition bowls are covered with preservative films, the nutrition bowls are put in a stainless steel iron pan, then the stainless steel iron pan is put in a culture container to be cultured for 15-20 days, then the preservative films are removed, culture continues for 5-7 days, 4 seedlings with good growth vigor are reserved, and the other seedlings are picked off. After sowing is completed, culture is carried out for 3-4 weeks under condition of the photoperiod of 10-hour illumination and 14-hour darkness, then culture continues under the condition of the photoperiod of 16-hour illumination and 8-hour darkness, and the strong arabidopsis seedlings are obtained. Common materials are adopted, the cultivating steps of arabidopsis are simplified, the cost is reduced, the time is shortened, and the strong seedling rate of the arabidopsis is increased.

The invention discloses a saline-alkaline tolerant growth-promoting bacterium YS-AT1 capable of secreting IAA and an application thereof. The bacterium belongs to Klebslella and is preserved in the China Center for Type Culture Collection on March 23, 2021, and the preservation number is M2021237. The bacterium has strong salt resistance and alkali resistance, the growth tolerant NaCl concentration of the bacterium is larger than 9% (w/v), the tolerant pH value of the bacterium is 8.0-12.0, the bacterium has certain alkali reduction capacity under different pH growth conditions, and the alkali reduction range under the different pH growth conditions is 2.7%-13.8%. Besides, the capability of the bacterium for secreting IAA under the induction of L-tryptophan can reach 5.38 [mu] g.mL<-1>. In addition, the bacterium has an obvious promotion effect on wheat seed germination and arabidopsis thaliana seedling growth under saline and alkaline conditions. Therefore, the bacterium has important application value in the aspects of preparation of growth-promoting microbial bacterium for saline-alkali areas and the like.

The invention discloses an application of 3,5-dichloroanthranilic acid in induction resistance of arabidopsis thaliana to gray mold and a method of the 3,5-dichloroanthranilic acid, and belongs to thefield of induction of plant resistance by compounds. According to the method, after a 3,5-dichloroanthranilic acid aqueous solution is subjected to root irrigation or page spraying treatment on the cultivated arabidopsis thaliana of all ages for two days, botrytis cinerea is inoculated, and the result shows that arabidopsis thaliana seedlings treated with 3,5-dichloroanthranilic acid show that the resistance to botrytis cinerea is obviously improved; the 3,5-dichloroanthranilic acid induced arabidopsis thaliana has the advantages of being good in water solubility, small in use amount, safe, green, cheap, easy to obtain and the like when used for inducing the arabidopsis thaliana to generate resistance to gray mold.

The invention discloses an application of alkaloid atropine in relieving low-temperature freeze injury stress of plants. The cold resistance of the plants can be improved through exogenous alkaloid atropine when cold injury occurs. Under the low-temperature stress condition, an alkaloid atropine aqueous solution with the concentration of 50-150 nmol/plant can be set for rape seedling root soakingtreatment, and freezing injury stress of rape is relieved; and under the low-temperature stress condition, an alkaloid atropine aqueous solution with the concentration of 10-30 nmol/plant can be set for arabidopsis thaliana seedling irrigation treatment, and the effect of relieving freezing injury stress of arabidopsis thaliana is achieved. Alkaloid atropine treatment is adopted to relieve damageof low temperature to the plant seedling stage, and a new way is opened up for guaranteeing safe overwintering of oilseed rape seedlings.

The invention discloses application of caproic acid in inducing resistance of arabidopsis thaliana to Pst DC3000 and a method thereof, and belongs to the field of plant resistance induced by compounds. According to the method, after root irrigation or page spraying treatment is conducted on cultivated four-week-old arabidopsis thaliana for two days through a caproic acid aqueous solution, Pst DC3000 is inoculated, and the result shows that arabidopsis thaliana seedlings treated with caproic acid show obviously improved resistance to Pst DC3000. Caproic acid induces arabidopsis thaliana to generate resistance to Pst DC3000 and has the advantages of being good in water solubility, small in usage amount, safe, green, cheap, easy to obtain and the like.