Arabidopsis thaliana salt-tolerance gene SRAT2 and uses thereof
A salt-tolerant gene, the technology of Arabidopsis thaliana, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of no commercial application, etc., and achieve the effect of overcoming continuous cropping obstacles, improving the salt tolerance of seedlings, and increasing farmers' income
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[0020] Gene acquisition
[0021] Using wild-type Arabidopsis thaliana Col-0 (Columbia ecotype) 7-day-old seedlings as materials, they were transferred to MS or MS+150mM NaCl medium for 24 hours, and then the aboveground parts were transferred to 1.5ml centrifuge tubes, and added Quickly ground with liquid nitrogen, then added 1ml Trizol (Invitrogen) and inverted to mix well, left at room temperature for 5min, and centrifuged at 12000rpm at 4°C for 10min. Transfer the supernatant to a new 1.5ml centrifuge tube, then add 200μl chloroform, shake vigorously for 15s, place at room temperature for 2-3min, and centrifuge at 12000rpm at 4°C for 15min. Aspirate the upper aqueous phase, add 500 μl isopropanol, and centrifuge at 12000 g for 10 min at 4 °C. Discard the supernatant, add 1.2ml of 75% ethanol, and centrifuge at 10000g for 5min at 4°C. Discard the supernatant, suck up the residual liquid, and dry at room temperature for 5-10min. Add 200 μl of DEPC (diethylpyrocarbonate) wate...
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