A low-virulence strain of Sclerotinia sclerotiorum carrying virus and its application in biological control

A technology for Sclerotinia sclerotiorum, plant pathogenic bacteria, applied in the fields of pest control, microorganism-based methods, chemicals for biological control, etc., can solve problems such as difficulties in plant disease control

Active Publication Date: 2021-09-14
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bioactive compounds are widely used in human medical health and agricultural production. Although fungal secondary metabolites can play a key role in the interaction between pathogenic microorganisms and hosts, the use of fungal secondary metabolites for plant Disease control is still difficult

Method used

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  • A low-virulence strain of Sclerotinia sclerotiorum carrying virus and its application in biological control
  • A low-virulence strain of Sclerotinia sclerotiorum carrying virus and its application in biological control
  • A low-virulence strain of Sclerotinia sclerotiorum carrying virus and its application in biological control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Screening, identification and characteristics of the low-virulence strain 767R45 of Sclerotinia sclerotiorum

[0037] 1) Screening and identification of strains of S. sclerotiorum low-virulence strain 767R45

[0038] S. sclerotiorum 767R45 was isolated from the sclerotia in rapeseed stalks of Sclerotinia sclerotiorum in Sichuan Province. Due to the abnormal shape of the original strain 767, high-throughput sequencing was carried out on the strain and it was found that the original strain 767 carried 6 different ssRNA viruses. However, due to the slow growth of the original strain 767, protoplast regeneration was carried out on it, and finally obtained A protoplast regeneration strain 767R45 with moderate growth rate and biocontrol potential.

[0039] The mycelia of 767R45 cultured for 5 days were scraped off from the PDA medium covered with cellophane, put into a mortar, added an appropriate amount of liquid nitrogen and ground thoroughly, and the total DNA of the 767R...

Embodiment 2

[0055] Volatile Gas Produced by Sclerotinia Sclerotiorum 767R45 Strain Inhibits Mycelia Growth of Various Pathogenic Fungi

[0056] Using two different methods, the two-partition dish method and the plate buckle method, the volatile gas produced by the sclerotinia 767R45 strain was used to analyze the effect of the phytopathogenic fungi Sclerotinia sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum , Helminthosporium oryzae, black shank of rapeseed and sour rot of citrus.

[0057] 1) Two-partition plate method: first inoculate mycelial blocks of Sclerotinia sclerotiorum 767R45 strain on one side of a 90mm PDA two-partition plate, and after culturing at 20°C for 5 days, inoculate fresh mycelial blocks of pathogenic fungi on the other side, and seal them with a parafilm. Each treatment was repeated three times, and the blank PDA was used as a control, and cultured at 20°C until the control was half-covered, the diameter of the pathogenic fungal colony was mea...

Embodiment 3

[0068] VOCs produced by Sclerotinia 767R45 strain inhibited the occurrence of strawberry gray mold, navel orange penicillium and navel orange green mold during storage

[0069] Select fresh, healthy fruits with the same size, wash them twice with distilled water, and then dry the surface naturally. Place strawberry fruits in a petri dish with a diameter of 60 mm and inoculate botrytis cinerea mycelia. Add 10 μL dropwise to a concentration of 2×10 7 spores / mL of Penicillium italicum / Penicillium digitatum spore suspension. The 400g solid culture (the sorghum solid culture of sclerotinia 767R45 bacterial strain) prepared by embodiment 1 is placed on the bottom of the desiccator, then puts into a ceramic partition, and the strawberry / navel orange that has been inoculated with the pathogenic bacteria spore liquid is put into the drying immediately Apply vaseline on the edge of the lid, twist it and close the lid tightly to prevent gas leakage, put the same mass of sterile sorghum ...

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Abstract

The invention relates to the technical field of biological control of plant diseases, and discloses a virus-carrying low-toxic strain of Sclerotinia sclerotiorum and its application in biological control. The strain is Sclerotinia 767R45, and the preservation number is: CCTCC NO: M2020419 . Volatile substances produced by S. sclerotiorum 767R45 strain and 10% fermentation filtrate can inhibit S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Helminthes oryzae, and black shank of rape and mycelial growth of Sour rot citri. In addition, the volatile substances and fermentation filtrate produced by Sclerotinia 767R45 can inhibit the occurrence of gray mold, penicillium and green mold during storage, and the fermentation filtrate can also inhibit the occurrence of Sclerotinia sclerotiorum, and its volatile Substances can promote the growth of Arabidopsis seedlings. The invention turns plant pathogenic bacteria into beneficial biological control resources, and provides a new perspective and new means for the biological control of crop fungal diseases.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, specifically comprising a kind of high-efficiency, broad-spectrum, resistant to various plant pathogenic fungi, and multi-virus compound infection. application to growth. [0002] technical background [0003] The occurrence and prevalence of plant diseases are the result of the interaction between host plants and pathogens, and crop diseases have seriously affected my country's economic benefits and people's lives. The use of chemical agents is still the main method for the prevention and control of crop diseases. However, the excessive use of chemical pesticides will seriously pollute agricultural products and the ecological environment. Therefore, it is particularly important to find new ways to prevent and control plant diseases safely and effectively. Biological control is in line with the national strategy of "two reductions" and green and sustainable developme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00A01P1/00A01P21/00A23B7/155A23B7/152C12R1/645
CPCA23B7/152A23B7/155A23V2002/00A01N63/30C12N1/145C12R2001/645A23V2200/10
Inventor 谢甲涛刘志军姜道宏付艳苹程家森郑丹
Owner HUAZHONG AGRI UNIV
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