Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for optimizing vitrification ultra-low temperature preservation effect of arabidopsis seedlings

A vitrification ultra-low temperature, Arabidopsis technology, which is applied in horticultural methods, plant preservation, botanical equipment and methods, etc., to achieve the effects of remarkable preservation effect, improved preservation effect, and optimized preservation effect.

Inactive Publication Date: 2015-01-07
SHANGHAI JIAO TONG UNIV
View PDF2 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adding certain exogenous substances in the existing technology is beneficial to improve the survival rate of cryopreservation, but it is unknown which exogenous substances to choose for specific plants and tissues to improve their preservation effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for optimizing vitrification ultra-low temperature preservation effect of arabidopsis seedlings
  • Method for optimizing vitrification ultra-low temperature preservation effect of arabidopsis seedlings
  • Method for optimizing vitrification ultra-low temperature preservation effect of arabidopsis seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1) Acquisition of Arabidopsis seedlings: Disinfect Arabidopsis seeds with 70% ethanol for 15 seconds, rinse them with sterile water for 4 times, then disinfect them with an aqueous solution containing 20 wt% NaClO and 0.01 wt% Tween 20 for 10 min, rinse them with sterile water 6 times; then the sterilized seeds were vernalized at 4°C for 48 hours and cultured on MS solid medium. 2 , cultivated at a temperature of 25° C.; then cultivated in the dark for 16 h at a culture temperature of 20° C., and cultured the seeds for a total of 60 h to obtain Arabidopsis seedlings with a seedling age of 60 h.

[0054] 2) Divide the 60h Arabidopsis seedlings into the experimental group and the control group, and set up 3 parallel experiments in each group, put 50 seedlings into a 2mL cryovial containing 1mL loading solution, and treat them at room temperature for 20min; Remove by suction, add vitrification solution, and treat at 0°C for 50 minutes; put the cryovial into liquid nitrogen...

Embodiment 2

[0066] This example is mainly to test and analyze the parameters of Arabidopsis thaliana seedlings at various stages of cryopreservation, so as to demonstrate the regulation of exogenous graphene quantum dots on membrane damage and oxidative stress during 60h cryopreservation of Arabidopsis seedlings.

[0067] 1) Experimental material: the material in group 1 is: the seedling of Arabidopsis thaliana germinated according to the method described in Example 1 of the present invention for 60h

[0068] Materials in group 2 are: seedlings treated with vitrification solution in Example 1;

[0069] Group 3 is: the seedlings after thawing after vitrification cryopreservation according to the method in Example 1;

[0070] Group 4 is the seedling after washing with washing liquid in embodiment 1;

[0071] Group 5 is the seedlings after recovery culture treatment in Example 1.

[0072] 1) Experimental materials: Arabidopsis thaliana seedlings germinated for 60 hours and seedlings after ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for optimizing a vitrification ultra-low temperature preservation effect of arabidopsis seedlings. According to the invention, the arabidopsis seedlings are processed by adopting a vitrification solution which contains a carbon nanometer material so as to improve the preservation effect of the arabidopsis seedlings. The method specifically comprises the steps of loading liquid treatment, vitrification solution treatment and liquid nitrogen preservation, wherein the vitrification solution is the MS culture solution containing 0.1-0.5 g / L of grapheme quantum dots. The method disclosed by the invention optimizes the preservation effect of the arabidopsis seedlings remarkably; as the grapheme quantum dots are added to serve as an allogenic material, the vitrification ultra-low temperature preservation of plants is facilitated.

Description

technical field [0001] The invention relates to the field of preservation of plants or parts thereof, in particular to a method for optimizing the effect of vitrification cryopreservation of Arabidopsis seedlings. Background technique [0002] Cryopreservation is a modern in vitro preservation technology for germplasm resources developed in the 1970s. Usually stored in liquid nitrogen, the substance metabolism and growth activities in the preserved material cells are almost completely stopped, and they are in a relatively stable biological state, achieving the purpose of long-term preservation of germplasm. Ultra-low temperature preservation is currently the only method that does not require continuous subculture. Medium and long-term preservation method. Vitrification cryopreservation is to place cells or tissues in a vitrification solution composed of a certain proportion of permeable and non-permeable protective agents, so that the material and its vitrification solution...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N3/00A01H4/00
Inventor 任丽陈冠群申晓辉张荻
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com