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Arabidopis thaliana salt tolerance gene SRAT1 and uses thereof

A salt-tolerant gene, Arabidopsis technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of no commercial application, and achieve the effect of overcoming the obstacles of continuous cropping, improving the salt tolerance of seedlings, and improving utilization

Inactive Publication Date: 2008-05-21
HANGZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, genetic engineering methods have been used at home and abroad to introduce genes involved in the synthesis of osmotic adjustment substances and ion regionalization effects into rice, corn, strawberry, tobacco and other crops, and high salt-tolerant crop lines have been obtained, but there is no commercial application yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Gene acquisition

[0020]Using wild-type Arabidopsis thaliana Col-0 (Columbia ecotype) 7-day-old seedlings as materials, they were transferred to MS or MS+150mM NaCl medium for 24 hours, and then the aboveground parts were transferred to 1.5ml centrifuge tubes, and added Quickly ground with liquid nitrogen, then added 1ml Trizol (Invitrogen) and inverted to mix well, left at room temperature for 5min, and centrifuged at 12000rpm at 4°C for 10min. Transfer the supernatant to a new 1.5ml centrifuge tube, then add 200μl chloroform, shake vigorously for 15s, place at room temperature for 2-3min, and centrifuge at 12000rpm at 4°C for 15min. Aspirate the upper aqueous phase, add 500 μl isopropanol, and centrifuge at 12000 g for 10 min at 4 °C. Discard the supernatant, add 1.2ml of 75% ethanol, and centrifuge at 10000g for 5min at 4°C. Discard the supernatant, suck up the residual liquid, and dry at room temperature for 5-10min. Add 200 μl of DEPC (diethylpyrocarbonate) wat...

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PUM

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Abstract

The invention provides and discloses an Arabidopsis thaliana salt-tolerant gene SRAT1, which has the base sequence described in SEQ ID NO.1 in the sequence listing. Transforming the Arabidopsis thaliana salt-tolerant gene SRAT1 into the model plant Arabidopsis can improve the salt tolerance of Arabidopsis seedlings. If the Arabidopsis salt-tolerant gene SRAT1 is transformed into plants such as rice or strawberry and other vegetables and flowers, it is possible to improve the salt tolerance of plants. Utilization of saline-alkali land; on the other hand, it can overcome continuous cropping obstacles caused by soil back-salt under facility conditions, improve the yield and quality of vegetables, flowers and other plants, and increase farmers' income.

Description

technical field [0001] The invention relates to the field of functional genomics, in particular to an Arabidopsis salt-tolerant gene SRAT1 and its application. Background technique [0002] The threat of soil salinization to agriculture is a global problem. There are a total of 1 billion hectares of saline-alkali land in the world, accounting for about 7.6% of the world's land area. my country's saline-alkali land is nearly 100 million hectares, and nearly 3.335 million hectares of agricultural land has been reduced or abandoned due to salinization. In recent years, my country's facility agriculture has developed rapidly, especially the production area of ​​vegetable and flower greenhouses has continued to expand. According to statistics, in 2005, the facility cultivation area of ​​vegetables, flowers, melons and fruits in China reached 2.1 million hectares. The development of facility agriculture has played an important role in the adjustment of agricultural production str...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415
Inventor 阮松林马华升王世恒忻雅钱丽华童建新王淑珍
Owner HANGZHOU ACAD OF AGRI SCI
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