Arabidopis thaliana salt tolerance gene SRAT1 and uses thereof
A salt-tolerant gene, Arabidopsis technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of no commercial application, and achieve the effect of overcoming the obstacles of continuous cropping, improving the salt tolerance of seedlings, and improving utilization
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[0019] Gene acquisition
[0020]Using wild-type Arabidopsis thaliana Col-0 (Columbia ecotype) 7-day-old seedlings as materials, they were transferred to MS or MS+150mM NaCl medium for 24 hours, and then the aboveground parts were transferred to 1.5ml centrifuge tubes, and added Quickly ground with liquid nitrogen, then added 1ml Trizol (Invitrogen) and inverted to mix well, left at room temperature for 5min, and centrifuged at 12000rpm at 4°C for 10min. Transfer the supernatant to a new 1.5ml centrifuge tube, then add 200μl chloroform, shake vigorously for 15s, place at room temperature for 2-3min, and centrifuge at 12000rpm at 4°C for 15min. Aspirate the upper aqueous phase, add 500 μl isopropanol, and centrifuge at 12000 g for 10 min at 4 °C. Discard the supernatant, add 1.2ml of 75% ethanol, and centrifuge at 10000g for 5min at 4°C. Discard the supernatant, suck up the residual liquid, and dry at room temperature for 5-10min. Add 200 μl of DEPC (diethylpyrocarbonate) wat...
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